Identification and characterization of conserved microRNAs and their target genes in wheat (Triticum aestivum)

Yin, Zhiqiu; Shen, Fafu

doi:10.4238/vol9-2gmr805

Cited by 44 publications

(28 citation statements)

References 29 publications

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“…Plants cannot escape from their surroundings; therefore, to adapt to changing environmental factors and respond to a variety of stressful conditions, plants must adjust the expression of a variety of genes at the transcriptional, post-transcriptional, and posttranslational levels to enhance their tolerance at the biochemical and physiological levels (McKenzie et al, 2007). Several hundred stress-responsive genes have been identified in plants to date (Shukla et al, 2008), and microRNAs (miRNAs) have emerged as important post-transcriptional regulators in plant stress responses (Yin and Shen, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identification of UV-B-induced microRNAs in wheat

Wang¹,

Sun²,

Song³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. MicroRNAs (miRNAs) play critical roles in posttranscriptional gene regulation and act as important endogenous regulators to various stressors. Ultraviolet-B (UV-B) radiation is a major factor influencing crop growth and development. In this study, we isolated a novel wheat miRNA, named Tae-miR6000, and confirmed its expression diversity after UV-B treatments. Additionally, using the Northern blotting technique, we found that six miRNAs were highly responsive to UV-B stress in wheat. Of these six miRNAs, miR159, miR167a, and miR171 were significantly upregulated, and the remaining three miRNAs were downregulated, at different time points after UV-B treatment. This result indicates that miRNAs may be involved in the regulation of targets after induction by UV-B stress. Furthermore, promoter analysis of the UV-B-responsive miRNA genes revealed some light-relevant cis-elements, such as the I-box and G-box. Taken together, the results of this study suggest that wheat miRNAs play important roles in the response to UV-B stress.

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Section: Introductionmentioning

confidence: 99%

Identification of UV-B-induced microRNAs in wheat

Wang¹,

Sun²,

Song³

et al. 2013

Genet. Mol. Res.

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“…In this study, 62 potential wheat miRNAs belonging to 30 miRNA families were identified. Given that many wheat miRNAs have been identified by high-throughput sequencing or computational approaches, the mature sequences of the 62 identified miRNAs were compared with published wheat miRNAs (Yao et al, 2007;Dryanova et al, 2008;Jin et al, 2008;Han et al, 2009;Wei et al, 2009;Yin and Shen, 2010). The results indicated that 14 miRNAs had an identical sequence with previously reported wheat miRNAs, including miR159, miR169, miR171, miR319, miR395a-c, miR398, miR408, miR444, miR1120, miR1128, and miR1135.…”

Section: Identification Of Potential Wheat Mirnas By Est Analysismentioning

confidence: 95%

Identification of miRNAs and their targets in wheat (Triticum aestivum L.) by EST analysis

Han¹,

Kong²,

Xie³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. MicroRNAs (miRNAs) are a newly discovered class of noncoding small RNAs that regulate gene expression by directing target mRNA cleavage or translational inhibition. A large number of miRNAs have been identified in plants. Increasing evidence has shown that miRNAs play multiple roles in plant biological processes. So far, identification of miRNAs has been limited to a few model plant species, whose genomes have been sequenced. Wheat (Triticum aestivum L.) is one of the most important cereal crops worldwide. To date, only a few conserved miRNAs have been predicted in wheat. Here, we showed the conserved miRNAs identified in wheat by expressed sequence tag (EST) analysis. All previously known miRNAs from Arabidopsis, rice, and other plant species were used in a BLAST search against the wheat EST database to identify novel wheat miRNAs by a series of filtering criteria. By this strategy, we identified 62 conserved miRNAs, belonging to 30 miRNA families, 48 of which were newly discovered in wheat. These newly identified wheat miRNAs may regulate 287 potential targets, which are involved in development, signal transduction, metabolic pathways, disease resistance, ion transportation, and environmental stress response.

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“…Later, they expanded this approach to other nucleotide sequences, including genome survey sequence (GSS) (Pan et al 2007). Currently, this approach has been widely adopted to identify plant miRNAs in many plant species, including maize (Zhang et al 2006a), soybean (Zhang et al 2008), wheat (Yin and Shen 2010), oilseed ), switchgrass (Xie et al 2010), tomato (Yin et al 2008), and apple (Gleave et al 2008). …”

Section: Comparative Genome-based Approachmentioning

confidence: 99%

MicroRNAs in Cotton

2011

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MicroRNAs (miRNAs) are an extensive class of newly discovered small regulatory RNAs with 20-24 nucleotides in length. Since it was first discovered in plants in 2002, it has been attracting the attention of many plant scientists because of its importance in plant growth and development. However, most of our knowledge about plant miRNAs comes from the investigation of model plant species, including Arabidopsis and rice. Only very recently, several reports have been published on the identification and expression analysis of miRNAs in cotton. Based on the conservation and unique characteristics of miRNAs, several tens of miRNAs have been predicted by comparative genome-based EST/GSS analysis. The next-generation deep-sequencing technologies, such as Solexa, provide a new platform to identify cotton miRNAs,

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Identification and characterization of conserved microRNAs and their target genes in wheat (Triticum aestivum)

Cited by 44 publications

References 29 publications

Identification of UV-B-induced microRNAs in wheat

Identification of UV-B-induced microRNAs in wheat

Identification of miRNAs and their targets in wheat (Triticum aestivum L.) by EST analysis

MicroRNAs in Cotton

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