Identification and characterization of common bean (Phaseolus vulgaris L.) lines well nodulated in the presence of high nitrate

Abstract

Common bean, Phaseolus vulgaris L., is known to be 'inefficient' in nodulation and N2 fixation although it responds to applied nitrogen. An experiment was conducted to identify and to characterize bean cultivars nodulating in the presence of a high level of nitrogen.Sixteen cultivars and a check for inefficient nodulation, OAC Seaforth, were inoculated and grown for 40 days in replicated pots supplied with zero, 3.5 and 10.5 mM combined nitrogen as NO3 and NH2. Seven traits relating to nodulation and N2 fixation were all significantly affected by N level (N), cultivar (Cv) and N x Cv interactions (except for root dry weight), indicating that cultivars responded differently to the N treatments. Total dry weight (W) and shoot to root ratio (S/R) increased with the increased N levels. Nodule dry weight (Wn), visual nodulation score (Nv) and nodulation index (Nx) decreased as the N increased. Percent N and N content per plant increased with the increased N level. Plant weight (W) was positively correlated with Wn, Nv and N content and negatively correlated with %N. Nodulation score was positively associated with Wn and plant N content. Genotypes superior in nodulation and N2 fixation in the presence of N were identified. Cultivars Italian Barlotti, California Light Red Kidney, Kentucky Wonder A and Pueblo 152 were selected for further testing and use in improving the nitrate tolerant nodulating characteristic of bean.

Enhancement of Biological Nitrogen Fixation of Common Bean in Latin America

“…Mineral nitrogen also caused elevated specific acetylene reducing activity in nodules at R 7 growth stage (Table 2). These results confirm positive effects of starter-N application on nodulation as described previously (Park and Buttery, 1989;Srivastava and Ormond, 1986). A non-nodulating soybean was included as a reference plant in this study to differentiate between N taken up from the substrate and N2 fixed.…”

“…Evaluation of segregating progenies and of advanced lines should be on low-N soils representative of those that prevail in the target production area. Both the rhizobia and plant genotypes often are sensitive to high levels of combined N, with N2 fixation often being suppressed by added fertilizer N. There is evidence for both natural variants and induced mutants (Park and Buttery, 1989;St. Clair et at., 1988) of common bean able to tolerate combined N. This characteristic along with the capacity to fix large amounts of N 2 should be bred into high-quality, well-adapted lines with acceptable seed traits.…”

Características adaptativas da associação simbiótica e da fixação biológica do nitrogênio molecular em plantas jovens de Lonchocarpus muehlbergianus Hassl., uma leguminosa arbórea nativa do Cerrado

“…A variabilidade para tolerância ao NO 3 -existe dentro de populações naturais podendo ser induzida por mutagênese (Jacobsen & Feenstra 1984;Carroll et al 1985a,b;Betts & Herridge 1987;Herridge & Betts 1985Park & Buttery 1988;Hardarson & Atkins 2003) e utilizada para o melhoramento genético (Park & Buttery 1989;Lambe et al 2008). Forrester et al (2006) revisaram os artigos publicados sobre a produtividade de Eucaliptus globulus com espécies arbóreas fixadoras de nitrogênio e verificaram efeito positivo na produtividade primária em todos os casos.…”

Shoot-root control of nitrate tolerance of N2 fixation in spontaneously tolerant soybean lines: reciprocal grafting experiments

“…Inhibition of symbiotic nitrogen fixation by nitrate is common to all legumes with variations between and within species (Hardarson et al, 1984;Harper and Gibson, 1984;Jensen, 1987;Park and Buttery, 1989). The physiological mechanisms involved in this inhibition have not been completely elucidated (Vessey and Waterer, 1992;Parsons et al, 1993).…”