Identification and characterization of circular RNAs in chicken hepatocytes

Zhang, Li; Xu, Haidong; Wang, Zhang; Li, Ting; Guo, Jiali; Adu-Asiamah, Patricia; Leng, Qiying; An, Lilong; Liu, Yijie; Zhang, Xiquan; Zhao, Zhihui

doi:10.1016/j.ghir.2019.05.002

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…A total of 11276 circRNAs were generated, of which 6790 circRNAs were detected in the brain, 5712 in the pituitary, 4937 in the ovary and 4160 in the liver. Most of the putative circRNAs we identified in tongue sole were expressed at low levels, which is consistent with recent studies [ 3 , 24 ] and suggests little potential function of most circRNAs [ 28 , 29 , 30 , 31 ]. In the current study, most circRNAs were derived from exons of genes, which was similar to those in human organs [ 32 , 33 ].…”

Section: Discussionsupporting

confidence: 91%

Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis

Bao

Wang

et al. 2021

Biology

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CircRNAs are novel endogenous non-coding small RNAs involved in the regulation of multiple biological processes. However, little is known regarding circRNAs in ovarian development and maturation of fish. Our study, for the first time, provides the genome-wide overview of the types and relative abundances of circRNAs in tongue sole tissues during three ovarian developmental stages. We detected 6790 circRNAs in the brain, 5712 in the pituitary gland, 4937 in the ovary and 4160 in the liver. Some circRNAs exhibit tissue-specific expression, and qRT-PCR largely confirmed 6 differentially expressed (DE) circRNAs. Gene Ontology and KEGG pathway analyses of DE mRNAs were performed. Some DE circRNA parental genes were closely associated with biological processes in key signalling pathways and may play essential roles in ovarian development and maturation. We found that the selected circRNAs were involved in 10 pathways. RNase R digestion experiment and Sanger sequencing verified that the circRNA had a ring structure and was RNase R resistant. qRT-PCR results largely confirmed differential circRNA expression patterns from the RNA-seq data. These findings indicate that circRNAs are widespread in terms of present in production-related tissues of tongue sole with potentially important regulatory roles in ovarian development and maturation.

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Section: Discussionsupporting

confidence: 91%

Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis

Bao

Wang

et al. 2021

Biology

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“…Subsequently, they have also demonstrated that circSVIL, produced from exon 6 to 14 of SVIL could function as miR‐203 sponges to promote the proliferation and differentiation of myoblast by upregulating the mRNA level of c‐Jun proto‐oncogene and MEF2C in chicken (Ouyang, Chen, Li, et al, 2018). Zhang et al identified a circRNA named circGHR from GHR gene, which was expressed at a higher level in the nucleus than in the cytoplasm in HuaiXiang chicken from day 1 to 5 week of age, suggesting its roles of circRNAs in chicken development (Zhang, Xu, et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Biological functions of circRNAs and their progress in livestock and poultry

Huang

Wang

Zhang

et al. 2020

Reprod Domestic Animals

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CircRNAs are a class of newly discovered RNAs extensively expressed in eukaryotic cells (Jeck et al., 2013). Compared with conventional linear RNAs (containing 5′ and 3′ ends), circRNAs are covalently closed RNA molecules. It was recognized as a class of functional RNA that differs from miRNA, piRNA and lncRNA due to its character of circularity (Xu, Wu, Han, Zhao, & Song, 2017). CircRNAs were first found in RNA viruses as a viroid as early as in 1976 (Sanger, Klotz, Riesner, Gross, & Kleinschmidt, 1976). And then in 1986, it was also founded that the HDV, an animal virus, has a circRNA genome, which were considered to be a characteristic of viroids (Kos, Dijkema, Arnberg, van der Meide, & Schellekens, 1986). Due to limitation of technology at that time, circRNAs were less reported and were once considered as by-products of mis-splicing or regarded as a meaningless abnormally cut RNA, which never got any attention by some researchers because traditional molecular techniques employed in RNA analysis were not able to detect circRNAs because they did not have cap of 5′ end and tail of 3′ end (Jeck & Sharpless, 2014). It was not until 1991 that endogenous circRNAs were demonstrated in human cells, which originated from exons (Nigro et al., 1991). Since then, with advancement of bioinformatics analysis and RNA sequencing technologies, circRNA has attracted more and more attention of many scientists because it has been reported to be ubiquitously present in eukaryotic organisms and have longer half-lives than linear RNAs (Memczak et al., 2013;

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“…Huaixiang chicken is a famous Chinese indigenous, yellow-feathered, slow-growing broiler breed that has become gradually favored by consumers because of the excellent meat quality [30][31][32]. However, its low egg-production rate has affected the breeding and breed-promotion of Huaixiang chickens.…”

Section: Introductionmentioning

confidence: 99%

Dietary Canthaxanthin Supplementation Promotes the Laying Rate and Follicular Development of Huaixiang Hens

Zhao,

Wu,

Liu

et al. 2023

Biology

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Canthaxanthin(CX) is a ketocarotenoid, which is widely used in poultry production as a lipophilic antioxidant. Huaixiang chickens are a local breed in China famous for their excellent meat quality; improving their laying rate via nutritional regulation has attracted extensive attention. The aim of this study was to evaluate the effects of dietary CX on the laying rate and follicular development in Huaixiang hens. A total of 180 Huaixiang hens were randomly divided into five groups with six replicates, and six chickens per replication. The control group (CON) were fed a basal diet, and the treatment group (NT) were fed a basal diet supplemented with 4, 6, 8 and 10 mg/kg CX. All chickens were 26 weeks old, living at an average environmental temperature of 25 ± 2 °C with a relative humidity of 65–75%. The results showed that supplementing the CX improved the laying rate and large white follicles (LWF) number (p < 0.05) and increased the concentration of reproductive hormones (LH, FSH, E2 and Prog) (p < 0.05), and the basal diet supplemented with 6 mg/kg CX worked best. Moreover, CX could increase the activities of antioxidant enzymes SOD and GSH-Px (p < 0.05) and reduce the content of the lipid peroxidation product MDA in Huaixiang chickens (p < 0.05); again, 6 mg/kg CX was best. In conclusion, dietary CX had positive effects on the laying rate, ovarian structure, reproductive hormone secretion, follicle development, and the antioxidant capacity of Huaixiang hens, and 6 mg/kg CX was recommended to be added to the diet of Huaixiang chickens.

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Identification and characterization of circular RNAs in chicken hepatocytes

Cited by 5 publications

References 22 publications

Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis

Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis

Biological functions of circRNAs and their progress in livestock and poultry

Dietary Canthaxanthin Supplementation Promotes the Laying Rate and Follicular Development of Huaixiang Hens

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