Identification and Characterization of circRNAs in Non-Lactating Dairy Goat Mammary Glands Reveal Their Regulatory Role in Mammary Cell Involution and Remodeling

Xuan, Rong; Wang, Jianmin; Li, Qing; Wang, Yanyan; Du, Shanfeng; Duan, Qingling; Guo, Yanfei; He, Ping; Ji, Zhibin; Chao, Tianle

doi:10.3390/biom13050860

Cited by 2 publications

(1 citation statement)

References 72 publications

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“…Furthermore, Xuan et al (2023) [ 104 ] aimed to explore the expression patterns of circRNAs in non-lactating goat mammary gland tissue, identifying those associated with mammary gland development and lactation regulation. They analyzed mammary gland tissue from three stages: late lactation (LL), dry period (DP), and late gestation (LG).…”

Section: Circular Rnas In Goat: Shaping Health and Productivitymentioning

confidence: 99%

Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep

Kirgiafini,

Kyrgiafini,

Gournaris

et al. 2024

Animals

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Circular RNAs (circRNAs) are unique noncoding RNA molecules, notable for their covalent closed-loop structures, which play a crucial role in regulating gene expression across a variety of biological processes. This review comprehensively synthesizes the existing knowledge of circRNAs in three key livestock species: Bos taurus (cattle), Ovis aries (sheep), and Capra hircus (goats). It focuses on their functional importance and emerging potential as biomarkers for disease detection, stress response, and overall physiological health. Specifically, it delves into the expression and functionality of circRNAs in these species, paying special attention to traits critical to livestock productivity such as milk production, meat quality, muscle development, wool production, immune responses, etc. We also address the current challenges faced in circRNA research, including the need for standardized methodologies and broader studies. By providing insights into the molecular mechanisms regulated by circRNAs, this review underscores their scientific and economic relevance in the livestock industry. The potential of circRNAs to improve animal health management and the quality of animal-derived products aligns with growing consumer concerns for animal welfare and sustainability. Thus, this paper aims to guide future research directions while supporting the development of innovative strategies in livestock management and breeding.

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Section: Circular Rnas In Goat: Shaping Health and Productivitymentioning

confidence: 99%

Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep

Kirgiafini,

Kyrgiafini,

Gournaris

et al. 2024

Animals

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Analysis of miRNAs in milk of four livestock species

Cendron,

Rosani,

Franzoi

et al. 2024

BMC Genomics

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Background Milk is essential for mammalian nutrition because it provides vital nutrients for growth and development. Milk composition, which is influenced by genetic and environmental factors, supports lactation, a complex process crucial for milk production and quality. Recent research has focused on noncoding RNAs, particularly microRNAs (miRNAs), which are present in body fluids and regulate gene expression post-transcriptionally. This study comprehensively characterizes miRNAs in milk of four livestock species, namely Bubalus bubalis, Capra hircus, Equus asinus, and Ovis aries and identifies potential target genes. Results High-throughput sequencing of milk RNA resulted in distinct read counts across species: B. bubalis (8,790,441 reads), C. hircus (12,976,275 reads), E. asinus (9,385,067 reads), and O. aries (7,295,297 reads). E. asinus had the highest RNA mapping rate (94.6%) and O. aries the lowest (84.8%). A substantially greater proportion of miRNAs over other small RNAs was observed for the donkey milk sample (7.74%) compared to buffalo (0.87%), goat (1.57%), and sheep (1.12%). Shared miRNAs, which included miR-200a, miR-200b, miR-200c, and miR-23a among others, showed varying expression levels across species, confirmed by qPCR analysis. Functional annotation of predicted miRNA target genes highlighted diverse roles, with an enrichment in functions linked to metabolism and immunity. Pathway analysis identified immune response pathways as significant, with several miRNAs targeting specific genes across species, suggesting their regulatory function in milk. Conclusions Both conserved and species-specific miRNAs were detected in milk of the investigated species. The identified target genes of these miRNAs have important roles in neonatal development, adaptation, growth, and immune response. Furthermore, they influence milk and meat production traits in livestock.

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Identification and Characterization of circRNAs in Non-Lactating Dairy Goat Mammary Glands Reveal Their Regulatory Role in Mammary Cell Involution and Remodeling

Cited by 2 publications

References 72 publications

Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep

Understanding Circular RNAs in Health, Welfare, and Productive Traits of Cattle, Goats, and Sheep

Analysis of miRNAs in milk of four livestock species

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