Identification and characterization of cholesterol esterase and lipase inhibitory peptides from amaranth protein hydrolysates

Ajayi, Feyisola Fisayo; Mudgil, Priti; Gan, Chee‐Yuen; Maqsood, Sajid

doi:10.1016/j.fochx.2021.100165

Cited by 25 publications

(19 citation statements)

References 45 publications

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“…D–2 showed more effective inhibition, and its inhibitory effect on CE increased as its concentration increased, with an IC50 value of 3.19 mg/mL (2.62 mM) ( Figure 6 b). Peptides with pancreatic CE inhibitory activity were obtained and predicted from camel milk and amaranth protein [ 2 , 19 , 51 ]. To date, no study has investigated the CE inhibitory activity of loach peptides in in vitro studies.…”

Section: Resultsmentioning

confidence: 99%

“…Antioxidant compounds are important for human health as they prevent or reduce excessive free radicals and reactive oxygen species (ROS) produced by the body, thus reducing damage to DNA, proteins, and lipids [ 1 , 2 , 3 ]. Antioxidants and enzyme inhibitors are employed as part of the control strategy for the management of healthy [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Metabolic diseases have always been a problem for modern people [ 5 ]. Among them, hypertension and hypercholesterolemia are metabolic disorders of the body, and oxidative stress is thought to be involved in their pathogenesis [ 2 , 3 , 5 ]. ROS are considered to be mediators of blood pressure caused by an angiotensin-converting enzyme (ACE) [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Multiple Bioactivities of Peptides from Hydrolyzed Misgurnus anguillicaudatus

Dou¹,

Wu²,

Xia³

et al. 2023

Molecules

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Misgurnus anguillicaudatus (loach) is a widely distributed benthic fish in Asia. In this study, the alkaline protease was used to hydrolyze loach, and the hydrolysate products of different molecular weights were obtained by membrane separation. In vitro antioxidant assays showed that the <3 kDa fraction (SLH-1) exhibited the strongest antioxidant activity (DPPH, hydroxyl radical and superoxide radical scavenging ability, and reducing power), while SLH-1 was purified by gel filtration chromatography, and peptide sequences were identified by LC-MS/MS. A total of six peptides with antioxidant activity were identified, namely SERDPSNIKWGDAGAQ (D-1), TVDGPSGKLWR (D-2), NDHFVKL (D-3), AFRVPTP (D-4), DAGAGIAL (D-5), and VSVVDLTVR (D-6). In vitro angiotensin-converting enzyme (ACE) inhibition assay and pancreatic cholesterol esterase (CE) inhibition assay, peptide D-4 (IC50 95.07 μg/mL, 0.12 mM) and D-2 inhibited ACE, and peptide D-2 (IC50 3.19 mg/mL, 2.62 mM), D-3, and D-6 acted as pancreatic CE inhibitors. The inhibitory mechanisms of these peptides were investigated by molecular docking. The results showed that the peptides acted by binding to the key amino acids of the catalytic domain of enzymes. These results could provide the basis for the nutritional value and promote the type of healthy products from hydrolyzed loach.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiple Bioactivities of Peptides from Hydrolyzed Misgurnus anguillicaudatus

Dou¹,

Wu²,

Xia³

et al. 2023

Molecules

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“…Moreover, numerous studies have demonstrated the potential of anti-hypercholesterolemic peptides derived using specific enzymes (alcalase, pepsin, trypsin, flavourzyme, chymotrypsin, etc.) in inhibiting CEase and PL active sites [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Plant-Protein (Quinoa) Derived Bioactive Peptides with Potential Anti-Hypercholesterolemic Activities: Identification, Characterization and Molecular Docking of Bioactive Peptides

Ajayi

Mudgil

Jobe

et al. 2023

Foods

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Hypercholesterolemia remains a serious global public health concern. Previously, synthetic anti-hypercholesterolemic drugs were used for ameliorating this condition; however, long-term usage presented several side-effects. In this regard, natural products as an adjunct therapy has emerged in recent times. This study aimed to produce novel bioactive peptides with anti-hypercholesterolemic activity (cholesterol esterase (CEase) and pancreatic lipase (PL)) from quinoa protein hydrolysates (QPHs) using three enzymatic hydrolysis methods (chymotrypsin, protease and bromelain) at 2-h hydrolysis intervals (2, 4, and 6 h). Chymotrypsin-generated hydrolysates showed higher CEase (IC50: 0.51 mg/mL at 2 h) and PL (IC50: 0.78 mg/mL at 6 h) inhibitory potential in comparison to other derived hydrolysates and intact quinoa proteins. Peptide profiling by LC-MS QTOF and in silico interaction with target enzymes showed that only four derived bioactive peptides from QPHs could bind in the active site of CEase, whereas twelve peptides could bind in the active site of PL. Peptides QHPHGLGALCAAPPST, HVQGHPALPGVPAHW, and ASNLDNPSPEGTVM were identified to be potential CEase inhibitors, and FSAGGLP, QHPHGLGALCAAPPST, KIVLDSDDPLFGGF, MFVPVPH, and HVQGHPALPGVPAHW were identified as potential PL inhibitors on the basis of the maximum number of reactive residues in these bioactive peptides. In conclusion, QPHs can be considered as an alternative therapy for the treatment of hypercholesterolemia.

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“…Recent work suggests that first and foremost peptides and protein hydrolysates derived from these beneficial species for the human health are worth considering [ 1 ]. The first study on an amaranth protein deriving bioactive peptide with cholesterol esterase and pancreatic lipase inhibitory activities was published in 2021 by Ajayi and colleagues [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

The Dual Nature of Amaranth—Functional Food and Potential Medicine

Baraniak

Kania-Dobrowolska

2022

Foods

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The beneficial health-promoting properties of plants have been known to mankind for generations. Preparations from them are used to create recipes for dietary supplements, functional food, and medicinal products. Recently, amaranth has become an area of increasing scientific and industrial interest. This is due to its valuable biological properties, rich phytochemical composition, and wide pharmacological activity. Amaranth is a pseudo-cereal crop with a dual character, combining the features of food and health-promoting product. This paper briefly and concisely reviews the current information on the chemical composition of amaranth, the value of its supplementation, the status of amaranth as a food ingredient as well as its key biological and pharmacological activities. The beneficial biological properties of amaranth preparations described in this paper may be an incentive to conduct further in-depth scientific research in this field and also to promote the development of innovative technologies in the food and cosmetics industry with the use of this plant.

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Identification and characterization of cholesterol esterase and lipase inhibitory peptides from amaranth protein hydrolysates

Cited by 25 publications

References 45 publications

Multiple Bioactivities of Peptides from Hydrolyzed Misgurnus anguillicaudatus

Multiple Bioactivities of Peptides from Hydrolyzed Misgurnus anguillicaudatus

Novel Plant-Protein (Quinoa) Derived Bioactive Peptides with Potential Anti-Hypercholesterolemic Activities: Identification, Characterization and Molecular Docking of Bioactive Peptides

The Dual Nature of Amaranth—Functional Food and Potential Medicine

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