Identification and Characterization of Cell Wall Proteins of a Toxic Dinoflagellate<i>Alexandrium catenella</i>Using 2-D DIGE and MALDI TOF-TOF Mass Spectrometry

Wang, Dazhi; Dong, Huicong; Li, Cheng; Xie, Zhang-Xian; Lin, Lin; Hong, Huasheng

doi:10.1155/2011/984080

Cited by 20 publications

(15 citation statements)

References 53 publications

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“…Protein spots were individually excised from gels and then manually in-gel trypsin digested as described previously (Wang et al, 2011). Briefly, each gel piece was destained, shrunk with 100% acetonitrile, and then air dried at room temperature.…”

Section: Ms and Database Searchmentioning

confidence: 99%

Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

et al. 2013

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The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on Nannochloropsis species have provided insights into the regulation of lipid production in response to nitrogen stress. Previous studies have focused on the responses of Nannochloropsis species to short-term nitrogen stress, but the effect of long-term nitrogen deprivation remains largely unknown. In this study, physiological and proteomic approaches were combined to understand the mechanisms by which Nannochloropsis oceanica IMET1 is able to endure long-term nitrate deprivation and its ability to recover homeostasis when nitrogen is amended. Changes of the proteome during chronic nitrogen starvation espoused the physiological changes observed, and there was a general trend toward recycling nitrogen and storage of lipids. This was evidenced by a global down-regulation of protein expression, a retained expression of proteins involved in glycolysis and the synthesis of fatty acids, as well as an up-regulation of enzymes used in nitrogen scavenging and protein turnover. Also, lipid accumulation and autophagy of plastids may play a key role in maintaining cell vitality. Following the addition of nitrogen, there were proteomic changes and metabolic changes observed within 24 h, which resulted in a return of the culture to steady state within 4 d. These results demonstrate the ability of N. oceanica IMET1 to recover from long periods of nitrate deprivation without apparent detriment to the culture and provide proteomic markers for genetic modification.

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Section: Ms and Database Searchmentioning

confidence: 99%

Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

et al. 2013

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“…The three 2-D gel images are separately taken using a confocal laser scanner, which are analyzed by computer-assisted overlay method. This technology, termed 2D differential gel electrophoresis (2D DIGE), allows a highly reproducible, quantitative analysis (Castillejo et al, 2011;Heywood et al, 2011;Wang et al, 2011).…”

Section: Robustnessmentioning

confidence: 99%

Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective

Magdeldin¹,

Zhang²,

Xu³

et al. 2012

Gel Electrophoresis - Principles and Basics

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“…In contrast to conventional biochemical approaches that address one or a few specific proteins at a time, proteomic techniques allow the simultaneous isolation and identification of hundreds or thousands of proteins in one sample. In the past few years, the proteomic approach has been used to study dinoflagellates, and a few important functional proteins have been identified and characterized [19][20][21][22][23].…”

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Proteomic analysis of a toxic dinoflagellate Alexandrium catenella under different growth phases and conditions

et al. 2012

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Alexandrium is a widely spread dinoflagellate genus throughout many regions of the world, which not only causes the harmful algal blooms (HABs) but also results in the paralytic shellfish poisoning (PSP) throughout the world. This study compared protein profiles of A. catenella grown under different growth phases and conditions using a proteomic approach, and identified the differentially expressed proteins. The results showed that the expressions of proteins identified in three different regions of the gels, the groups 1, 2 and 3 proteins, varied significantly with the growth phases and conditions. Group 1 proteins and six Group 2 proteins were highly expressed at the initial, exponential and stationary growth phases, eight Group 2 proteins were highly expressed only at the initial phase, and Group 3 proteins were highly expressed at the exponential and/or stationary phases. However, all these proteins were expressed at low levels or were barely visible at the dissipation phase. The expressions of groups 1 and 2 proteins were low or barely visible in various growth conditions except in continuous darkness they were highly expressed. Group 3 proteins, on the other hand, were overexpressed in continuous illumination and expressed at low levels or barely visible in continuous darkness or under nitrate-starvation. The data from MALDI-TOF-TOF mass spectrometry demonstrated that these differentially expressed proteins were associated with macromolecular biosynthesis, photosynthesis, tRNA synthesis and DNA stability, stress response and cell division regulation. Synthetase was the major component of the altered proteins. This is one of the first comprehensive proteomic study of a dinoflagellate, A. catenella, that provides a fundamental understanding of the proteins involved in A. catenella growth and response to environmental stresses, and potential physiological indicator proteins related to growth and environmental stress have been identified.

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Identification and Characterization of Cell Wall Proteins of a Toxic DinoflagellateAlexandrium catenellaUsing 2-D DIGE and MALDI TOF-TOF Mass Spectrometry

Cited by 20 publications

References 53 publications

Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

Two-Dimensional Polyacrylamide Gel Electrophoresis - A Practical Perspective

Proteomic analysis of a toxic dinoflagellate Alexandrium catenella under different growth phases and conditions

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