Identification and characterization of CD133<sup>+</sup>CD44<sup>+</sup> cancer stem cells from human laryngeal squamous cell carcinoma cell lines

Wang, Jue; Wu, Yongyan; Gao, Wei; Li, Fēi; Bo, Yunfeng; Zhu, Meixia; Fu, Rong; Liu, Qingqing; Wen, Shuxin; Wang, Binquan

doi:10.7150/jca.17444

Cited by 60 publications

(58 citation statements)

References 34 publications

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“…Our previous study showed that CD133+CD44+ cancer stem cells isolated from the LSCC cell lines Hep2 and TU-177 by magnetic-activated cell sorting (MACS) exhibited enhanced cell viability, migration and invasive capability, colony-formation ability, and resistance to chemotherapy and irradiation. Furthermore, in nude mice, tumorigenicity was higher for CD133+CD44+ LSCC stem cells than CD133+ or CD44+ LSCC stem cells [6]. Therefore, investigating the mechanisms underlying CD133+CD44+ LSCC cancer cells would help with treatment and prognosis of LSCC.…”

Section: Introductionmentioning

confidence: 99%

“…+ cells (hereafter TDP cells) and CD133-CD44-cells (hereafter TDN cells) were isolated from LSCC TU-177 cells, and spheroid-formation experiments were conducted as described [6]. Total RNA was extracted by using TRIzol reagent (Invitrogen, Carlsbad, CA) as the manufacturer instructed, followed by DNase I treatment to remove DNA contamination.…”

Section: Inhibition Of Cells With Cisplatinmentioning

confidence: 99%

“…Transwell chamber assay was performed for migration and invasion analysis of cells as described previously [6]. Briefly, cells were resuspended with culture medium containing 1% FBS (Biological Industries, Cromwell, CT).…”

Section: Migration and Invasion Assaymentioning

confidence: 99%

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Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Zhang²,

Niu³

et al. 2018

Cell Physiol Biochem

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Background/Aims: CD133+CD44+ cancer stem cells previously isolated from laryngeal squamous cell carcinoma (LSCC) cell lines showed strong malignancy and tumorigenicity. However, the molecular mechanism underlying the enhanced malignancy remained unclear. Methods: Cell proliferation assay, spheroid-formation experiment, RNA sequencing (RNA-seq), miRNA-seq, bioinformatic analysis, quantitative real-time PCR, migration assay, invasion assay, and luciferase reporter assay were used to identify differentially expressed mRNAs, lncRNAs, circRNAs and miRNAs, construct transcription regulatory network, and investigate functional roles and mechanism of circRNA in CD133+CD44+ laryngeal cancer stem cells. Results: Differentially expressed genes in TDP cells were mainly enriched in the biological processes of cell differentiation, regulation of autophagy, negative regulation of cell death, regulation of cell growth, response to hypoxia, telomere maintenance, cellular response to gamma radiation, and regulation of apoptotic signaling, which are closely related to the malignant features of tumor cells. We constructed the regulatory network of differentially expressed circRNAs, miRNAs and mRNAs. qPCR findings for the expression of key genes in the network were consistent with the sequencing data. Moreover, our data revealed that circRNA hg19_circ_0005033 promotes proliferation, migration, invasion, and chemotherapy resistance of laryngeal cancer stem cells. Conclusions: This study provides potential biomarkers and targets for LSCC diagnosis and therapy, and provide important evidences for the heterogeneity of LSCC cells at the transcription level.

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Section: Introductionmentioning

confidence: 99%

Section: Inhibition Of Cells With Cisplatinmentioning

confidence: 99%

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Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Zhang²,

Niu³

et al. 2018

Cell Physiol Biochem

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“…These cells are present in HNSCC, and overexpress CD44 and aldehyde dehydrogenase (ALDH) proteins, which are currently regarded as HNSCC CSCs' markers [127][128][129]. Moncharmont and colleagues reported that consecutive cell sorting was performed to isolate SQ20B/ CSCs from SQ20B parental population utilizing Side Population (SP).…”

Section: Carbon Ion Irradiation Counteracts Cancer Stem Cells' Migratmentioning

confidence: 99%

The impact of melatonin and carbon ion irradiation on cancer stem cells

Liu¹,

Reíter²

2017

Nucl Med Biomed Imaging

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Aim: Cancer stem cells (CSCs) exhibited an excessive migratory and invasive potential. Melatonin may inhibit multiple crucial signals associated with tumor stem cell self-renewal, viability, invasiveness, tumor growth, and therapy resistance. Carbon ion irradiation may be a promising therapeutic modality in both non-stem-like and stem-like tumor cells in contrast to photon irradiation. A comprehensive understanding of the mechanisms and molecular pathways associated with invasive properties of CSCs is essential in developing novel treatment options for cancer therapy that target CSCs. Materials and methods:A systematic review of the existing literature was conducted using the following search terms: 'melatonin', 'X-ray irradiation', 'charged particle irradiation', 'carbon ion irradiation', 'cancer stem cells', 'tumor-initiating cells' and 'cancer stem-like cells'. The search used PubMed and spanned the period from January 2000 to December 2016. Conclusion:Cancer stem cells possess the capacity of self-renewal and pluripotency, generating all cells within a tumor, and are responsible for tumor growth, therapy resistance and metastasis. Melatonin attenuated AKT activation, EZH2 S21 phosphorylation, EZH2-STAT3 interactions and altered histone modifications to reduce tumor initiation and propagation of brain tumor stem cells (BTSC). Melatonin increases the efficacy of chemotherapeutic agents, targeting both the tumor bulk and BTSCs through the regulation of the expression and function of the ABCG2/BCRP transporter by inducing the methylation of its promoter. Melatonin treatment induced cell death with ultrastructural characteristics of autophagy. Breast cancer stem cells (BCSCs) are responsive to melatonin treatment by way of reducing the viability and the invasiveness of breast cancer mammospheres as well as regulating the expression of OCT4, N-cadherin and vimentin proteins associated with epithelial mesenchymal transition in BCSCs. Carbon irradiation is effective in brain tumor stem cell (BTSC) elimination with relative biologic effectiveness (RBE) in the range of 1.87-3.44. Carbon ion irradiation may be a promising therapeutic modality because it reduces migration and invasion processes in both head and neck squamous cell carcinoma and cancer stem cells in contrast to photon irradiation. Low LET X-ray irradiation may essentially eradicate the non-stem-like tumor cells, consequently the radioresistant cancer stem-like cell population is obviously enriched. In contrast, carbon ion irradiation may eradicate both non-stemlike and stem-like tumor cells at the same time. Carbon ion irradiation is a promising tool to eradicate putative colon cancer stem-like cells. Further investigation to elucidate the mechanisms and molecular pathways involved in cancer stem cells particularly associated with melatonin and carbon ion irradiation certainly is warranted.

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“…CSCs are characterized by significant overexpression of certain cell surface markers and stemness genes CD133, a 120 kDa pentaspan transmembrane cell surface glycoprotein [16,17], is a commonly studied potential CSCs marker, which has been demonstrated to be expressed in distinct normal tissue stem cells [18]. It is an apical plasma membrane protein with potential to isolate stem cells from distinct tissues and tumors including LCa [19][20][21].…”

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confidence: 99%

Current cancer stem cell biomarkers in laryngeal cancer

Karataş¹,

Barlak²,

Şahin³

2017

The European Research Journal

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Larynx cancer (LCa), being an aggressive malignancy, is the second most commonly diagnosed malignant type of head and neck squamous cell carcinoma worldwide. Although there have been significant improvements in the detection and diagnosis of LCa in the last decades, it is still one of the considerable causes of cancer deaths and an urgent need for identification of novel biomarkers with diagnostic and prognostic significance still remains. The cancer stem cells (CSCs) resemble normal stem cells in terms of biological features and are considered to play critical roles in biological aggressiveness of tumors. Accumulating evidences have proven the existence of CSCs in various tumors including LCa and they are considered as driving force for tumor relapse, metastasis, and chemo-radioresistance. Comprehensive identification and characterization of the CSCs is of paramount importance for their further characterization to develop more effective and targeted therapeutic strategies against cancer. Here, we reviewed and summarized the most current literature to provide an insight into the functions and roles of current CSCs biomarkers in human LCa. We believe that this review will contribute to the knowledge of scientists especially working with LCa CSCs and will help understanding the significance of CSCs biomarkers implicated in LCa pathogenesis.

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Identification and characterization of CD133⁺CD44⁺ cancer stem cells from human laryngeal squamous cell carcinoma cell lines

Cited by 60 publications

References 34 publications

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

The impact of melatonin and carbon ion irradiation on cancer stem cells

Current cancer stem cell biomarkers in laryngeal cancer

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Identification and characterization of CD133+CD44+ cancer stem cells from human laryngeal squamous cell carcinoma cell lines

Cited by 60 publications

References 34 publications

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

Whole-Transcriptome Analysis of CD133+CD144+ Cancer Stem Cells Derived from Human Laryngeal Squamous Cell Carcinoma Cells

The impact of melatonin and carbon ion irradiation on cancer stem cells

Current cancer stem cell biomarkers in laryngeal cancer

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Identification and characterization of CD133⁺CD44⁺ cancer stem cells from human laryngeal squamous cell carcinoma cell lines