Identification and characterization of bioactive factors in bladder submucosa matrix

Chun, So Young; Lim, Grace J.; Kwon, Tae Gyun; Kwak, Eun Kyoung; Kim, Bup Wan; Atala, Anthony; Yoo, James J.

doi:10.1016/j.biomaterials.2007.05.020

Cited by 169 publications

(108 citation statements)

References 33 publications

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“…Additionally, the extent to which matricryptic peptides remain active in vivo is not known. Because ECM scaffolds consist of various molecules such as collagen and fibronectin, proteoglycans, glycoproteins, growth factors, and cytokines (46), degradation of these ECM scaffolds releases a heterogeneous set of molecules (13,21), each with varying biologic properties in vivo. Subsets of these peptides have been found to have different bioactive properties in vitro (13,17,19,44,47,48).…”

Section: Discussionmentioning

confidence: 99%

Epimorphic regeneration approach to tissue replacement in adult mammals

Agrawal

Johnson²,

Reing³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

141

134

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Urodeles and fetal mammals are capable of impressive epimorphic regeneration in a variety of tissues, whereas the typical default response to injury in adult mammals consists of inflammation and scar tissue formation. One component of epimorphic regeneration is the recruitment of resident progenitor and stem cells to a site of injury. Bioactive molecules resulting from degradation of extracellular matrix (ECM) have been shown to recruit a variety of progenitor and stem cells in vitro in adult mammals. The ability to recruit multipotential cells to the site of injury by in vivo administration of chemotactic ECM degradation products in a mammalian model of digit amputation was investigated in the present study. Adult, 6- to 8-week-old C57/BL6 mice were subjected to midsecond phalanx amputation of the third digit of the right hind foot and either treated with chemotactic ECM degradation products or left untreated. At 14 days after amputation, mice treated with ECM degradation products showed an accumulation of heterogeneous cells that expressed markers of multipotency, including Sox2, Sca1, and Rex1 (Zfp42). Cells isolated from the site of amputation were capable of differentiation along neuroectodermal and mesodermal lineages, whereas cells isolated from control mice were capable of differentiation along only mesodermal lineages. The present findings demonstrate the recruitment of endogenous stem cells to a site of injury, and/or their generation/proliferation therein, in response to ECM degradation products.

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Section: Discussionmentioning

confidence: 99%

Epimorphic regeneration approach to tissue replacement in adult mammals

Agrawal

Johnson²,

Reing³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

141

134

View full text Add to dashboard Cite

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“…Although the mechanisms by which these materials support and promote a constructive remodeling process are only partially understood, it appears clear that rapid degradation of the scaffold material with concurrent release of both intact growth factors and newly generated bioactive matricryptic peptides as well as the provision of unique surface architectures are factors that play an important role. [34][35][36][37][38][39][40][41] The ability to promote constructive remodeling in vivo has also been shown to be highly dependent on the methods used in preparing the scaffold material. 19,20,42 For example, chemical crosslinking is often used to increase the mechanical strength of a scaffold material, to slow degradation, or to mask cellular epitope that may remain within the scaffold material after decellularization.…”

Section: Figmentioning

confidence: 99%

Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix

Brown

Freund

Han

et al. 2011

Tissue Engineering Part C: Methods

191

197

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“…Both materials have been shown to be biocompatible and safely used clinically (11,12,34,35). Acellular tissue matrix, obtained from porcine bladders, was processed using a multiple-step detergent wash protocol developed in our laboratory (12,16) …”

Section: Preparation Of Composite Biomaterialsmentioning

confidence: 99%

“…Each type of biomaterials has desirable traits which are exclusive of the other. Acellular tissue matrices possess the desired biocompatibility (11)(12)(13), contain biomimetic factors (14)(15)(16)) that promote tissue development and have adhesion domain sequences (e.g., RGD) that may assist in retaining the phenotype and activity of many types of cells (17). These matrices are known to slowly degrade upon implantation and are usually replaced and remodeled by ECM proteins synthesized and secreted by transplanted or ingrowing cells (18)(19)(20)(21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Composite scaffolds for the engineering of hollow organs and tissues

Eberli¹,

Filho²,

Atala³

et al. 2009

Methods

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Composite scaffolds for the engineering of hollow organs and tissues AbstractSeveral types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. This scaffold system may be useful in patients requiring hollow organ replacement. Title: COMPOSITE SCAFFOLDS FOR THE ENGINEERING OF HOLLOW ORGANS AND TISSUES Authors Abstract:Several types of synthetic and naturally derived biomaterials have been used for augmenting hollow organs and tissues. However, each has desirable traits which were exclusive of the other. We fabricated a composite scaffold and tested its potential for the engineering of hollow organs in a bladder tissue model. The composite scaffolds were configured to accommodate a large number of cells on one side and were designed to serve as a barrier on the opposite side. The scaffolds were fabricated by bonding a collagen matrix to PGA polymers with threaded collagen fiber stitches. Urothelial and bladder smooth muscle cells were seeded on the composite scaffolds, and implanted in mice for up to 4 weeks and analyzed. Both cell types readily attached and proliferated on the scaffolds and formed bladder tissue-like structures in vivo. These structures consisted of a luminal urothelial layer, a collagen rich compartment and a peripheral smooth muscle layer. Biomechanical studies demonstrated that the tissues were readily elastic while maintaining their pre-configured structures. This study demonstrates that a composite scaffold can be fabricated with two completely different polymer systems for the engineering of hollow organs. The composite scaffolds are biocompatible, possess adequate physical and structural characteristics for bladder tissue engineering, and are able to form tissues in vivo. Thi...

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Identification and characterization of bioactive factors in bladder submucosa matrix

Cited by 169 publications

References 33 publications

Epimorphic regeneration approach to tissue replacement in adult mammals

Epimorphic regeneration approach to tissue replacement in adult mammals

Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix

Composite scaffolds for the engineering of hollow organs and tissues

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