Identification and characterization of bacteriocin biosynthetic gene clusters found in multiple bacteriocins producing Lactiplantibacillus plantarum PUK6

Kawahara, Ai; Zendo, Takeshi; Matsusaki, Hiromi

doi:10.1016/j.jbiosc.2022.01.008

Cited by 9 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These speciesspecific bacteriocins give them specific antimicrobial properties that play a significant role in food preservation e.g. plantaricins (Choi et al, 2021;Kawahara et al, 2022). Apart from bacteriocins, a larger family of T3PKS BGCs was observed across most species, encoding the enzyme hydroxymethylglutaryl-CoA synthase.…”

Section: Discussionmentioning

confidence: 99%

Pangenome Analysis Reveals the Genetic Basis for Taxonomic Classification of the Lactobacillaceae Family

Rajput

Chauhan

Mohite

et al. 2023

Preprint

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Pangenome Analysis Reveals the Genetic Basis for Taxonomic Classification of the Lactobacillaceae Family

Rajput

Chauhan

Mohite

et al. 2023

Preprint

View full text Add to dashboard Cite

“…The concentration of PGRPs in milk may vary depending on a number of variables, such as the camel's health, lactation stage, and season [59,60]. PGRPs are believed to inactivate bacterial pathogens by attaching peptidoglycan to their cell walls [35,61,62]. In addition, PGRPs may bind to both lipopolysaccharide and lipoteichoic acid and inhibit proinflammatory cytokines [63].…”

Section: Peptidoglycan Recognition Proteins (Pgrps)mentioning

confidence: 99%

Camel Milk: Antimicrobial Agents, Fermented Products, and Shelf Life

Hamed,

Mbye,

Ayyash

et al. 2024

Foods

View full text Add to dashboard Cite

The camel milk (CM) industry has witnessed a notable expansion in recent years. This expansion is primarily driven by the rising demand for CM and its fermented products. The perceived health and nutritional benefits of these products are mainly responsible for their increasing popularity. The composition of CM can vary significantly due to various factors, including the breed of the camel, its age, the stage of lactation, region, and season. CM contains several beneficial substances, including antimicrobial agents, such as lactoferrin, lysozyme, immunoglobulin G, lactoperoxidase, and N-acetyl-D-glucosaminidase, which protect it from contamination by spoilage and pathogenic bacteria, and contribute to its longer shelf life compared to bovine milk (BM). Nevertheless, certain harmful bacteria, such as Listeria monocytogenes, Yersinia enterocolitica, and Escherichia coli, have been detected in CM, which is a significant public health concern. Therefore, it is crucial to understand and monitor the microbial profile of CM and follow good manufacturing practices to guarantee its safety and quality. This review article explores various aspects of CM, including the types of beneficial and harmful bacteria present in it, the composition of the milk, its antimicrobial properties, its shelf life, and the production of fermented CM products.

show abstract

“…The bacteriocin-encoded gene detection found that bacteriocin type belonged to plantaricin (pln) which is a second type B bacteriocin with two peptides. Its activity is regarding the two distinct pep-tide actions (Barbosa et al, 2021;Kawahara et al, 2022). According to Zacharof & Lovitt (2012), L. plantarum generated at least six different bacteriocins.…”

Section: Bacteriocin-encoding Genesmentioning

confidence: 99%

Detection of Plantaricin-Encoding Gene and Its Partial Purification in Lactobacillus plantarum BP102

Suryani,

Jatmiko,

Mustafa

2023

Jurnal Biodjati

View full text Add to dashboard Cite

Lactobacillus plantarum BP102 isolated from garlic bulb tissue has probiotic properties, including producing bacteriocin called plantaricin. This study aimed to detect the gene encoding bacteriocin produced by Lactobacillus plantarum BP102, and to evaluate the bacteriocin activity at each stage of partial purification. After the end of the log phase of L. plantarum BP102 was determined, and the bacteriocin-encoding genes were checked by PCR technique. Partial purification of bacteriocin was elucidated including pH-neutralized cell-free-supernatant (CFS), precipitation using 80% of ammonium sulfate, and dialysis (cut-off 10 kDa), then the bacteriocin activity in every partial purification stage was evaluated. The molecular weight of plantaricin was estimated using SDS-PAGE analysis. Lactobacillus plantarum BP102 harbored the gene encoding plantaricin (pln) biosynthesis, namely plnEF and plnK genes. The activity of crude bacteriocin was inactivated by the presence of proteinase-K enzyme. The protein concentration was gradually decreased along with the purification process. The bacteriocin activity was demonstrated at each step of the purification process (CFS, precipitation, and dialysis) against Bacillus cereus by 9.23 ± 0.20 mm, 7.86 ± 0.15 mm, and 7.6 ± 0.10 mm, respectively; while, Escherichia coli by 10.3 ± 0.55 mm, 7.4 ± 0.1 mm, and 6.86 ± 0.45, respectively. The molecular weight of partially purified bacteriocin BP102 was found to be approximately 15.9 kDa. The overlaid part of the gel showed a slight inhibition against E. coli due to a low protein concentration. This bacteriocin purification process should be further optimized to improve the bacteriocin activity that could be useful for food preservation.

show abstract

Identification and characterization of bacteriocin biosynthetic gene clusters found in multiple bacteriocins producing Lactiplantibacillus plantarum PUK6

Cited by 9 publications

References 29 publications

Pangenome Analysis Reveals the Genetic Basis for Taxonomic Classification of the Lactobacillaceae Family

Pangenome Analysis Reveals the Genetic Basis for Taxonomic Classification of the Lactobacillaceae Family

Camel Milk: Antimicrobial Agents, Fermented Products, and Shelf Life

Detection of Plantaricin-Encoding Gene and Its Partial Purification in Lactobacillus plantarum BP102

Contact Info

Product

Resources

About