Identification and characterization of bacterial symbionts in three species of filth fly parasitoids

Betelman, Kfir; Caspi-Fluger, Ayelet; Shamir, Maayan; Chiel, Elad

doi:10.1093/femsec/fix107

Cited by 18 publications

(31 citation statements)

References 45 publications

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“…Such a Wolbachia ‐induced uniparental reproduction can be found in Apoanagyrus diversicornis (Hymenoptera: Encyrtidae), the closest relative of A. vladimiri that has been reported to carry Wolbachia 62 . Wolbachia is known to induce CI in many parasitoid species, 42,63 but does not seem to have this phenotype in our study system. Nevertheless, the possibility that Wolbachia may induce CI under other environmental conditions, or in other A. vladimiri populations cannot be ruled out (e.g., 64 ).…”

Section: Discussionmentioning

confidence: 59%

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Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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BACKGROUND Like numerous other animals, biocontrol agents (BCAs) of arthropod pests carry various microorganisms that may have diverse effects on the biology of their eukaryote hosts. We postulated that it is possible to improve the efficacy of BCAs by manipulating the composition of their associated microbiota. The parasitoid wasp Anagyrus vladimiri (Hymenoptera: Encyrtidae) from a mass‐rearing facility was chosen for testing this hypothesis. RESULTS High‐throughput sequencing analysis indicated that fungal abundance in A. vladimiri was low and variable, whereas the bacterial community was dominated by the endosymbiont Wolbachia. Wolbachia was fixed in the mass‐rearing population, whereas in field‐collected A. vladimiri Wolbachia's prevalence was only approximately 20%. Identification of Wolbachia strains from the two populations by Multi Locus Sequence Typing, revealed two closely related but unique strains. A series of bioassays with the mass‐rearing Wolbachia‐fixed (W+) and a derived antibiotic‐treated Wolbachia‐free (W–) lines revealed that: (i) Wolbachia does not induce reproductive manipulations; (ii) W– females have higher fecundity when reared individually, but not when reared with conspecifics; (iii) W+ females outcompete W– when they share hosts for oviposition; (iv) longevity and developmental time were similar in both lines. CONCLUSIONS The findings suggest that W+ A. vladimiri have no clear fitness benefit under mass‐rearing conditions and may be disadvantageous under lab‐controlled conditions. In a broader view, the results suggest that augmentative biological control can benefit from manipulation of the microbiome of natural enemies.

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Section: Discussionmentioning

confidence: 59%

“…To determine the percentage of individuals of both field and MR populations that carry Wolbachia , a minimum of 30 individual wasps from each population were homogenized as described in Betelman et al ., 42 without surface sterilization. Those samples were used as templates for the PCRs.…”

Section: Methodsmentioning

confidence: 99%

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Izraeli

Lalzar

Netanel

et al. 2020

Pest Management Science

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“…This initial characterization of Wolbachia and CI in Cu. pipiens opened the floodgates, with many ensuing studies reporting cases of CI-inducing Wolbachia in Diptera ( Baton et al, 2013 ; Bian et al, 2013 ; Hoffmann et al, 1986 ; Riegler and Stauffer, 2002 ), Hymenoptera ( Betelman et al, 2017 ; Dittmer et al, 2016 ), Coleoptera ( Kajtoch and Kotásková, 2018 ), Hemiptera ( Ju et al, 2017 ; Ramírez-Puebla et al, 2016 ), Orthoptera ( Martínez-Rodríguez and Bella, 2018 ), Lepidoptera ( Arai et al, 2019 ; Hornett et al, 2008 ), Thysanoptera ( Nguyen et al, 2017 ), Acari ( Gotoh et al, 2007 ; Gotoh et al, 2003 ; Vala et al, 2002 ), Isopoda ( Cordaux et al, 2012 ; Sicard et al, 2014 ), and Arachnids ( Curry et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Symbiont-mediated cytoplasmic incompatibility: What have we learned in 50 years?

Shropshire

Leigh

Bordenstein

2020

eLife

120

151

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Cytoplasmic incompatibility (CI) is the most common symbiont-induced reproductive manipulation. Specifically, symbiont-induced sperm modifications cause catastrophic mitotic defects in the fertilized embryo and ensuing lethality in crosses between symbiotic males and either aposymbiotic females or females harboring a different symbiont strain. However, if the female carries the same symbiont strain, then embryos develop properly, thereby imparting a relative fitness benefit to symbiont-transmitting mothers. Thus, CI drives maternally-transmitted bacteria to high frequencies in arthropods worldwide. In the past two decades, CI experienced a boom in interest due to its (i) deployment in worldwide efforts to curb mosquito-borne diseases, (ii) causation by bacteriophage genes, cifA and cifB, that modify sexual reproduction, and (iii) important impacts on arthropod speciation. This review serves as a gateway to experimental, conceptual, and quantitative themes of CI and outlines significant gaps in understanding CI’s mechanism that are ripe for investigation from diverse subdisciplines in the life sciences.

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“…This initial characterization of Wolbachia and CI in Cu. pipiens opened the floodgates, with many ensuing studies reporting cases of CI-inducing Wolbachia in Diptera (Baton et al, 2013;Bian et al, 2013;Hoffmann et al, 1986;Riegler and Stauffer, 2002), Hymenoptera (Betelman et al, 2017;Dittmer et al, 2016), Coleoptera (Kajtoch and Kotásková, 2018), Hemiptera (Ju et al, 2017;Ramírez-Puebla et al, 2016), Orthoptera (Martinez-Rodriguez and Bella, 2018), Lepidoptera (Arai et al, 2018;Hornett et al, 2008), Thysanoptera (Nguyen et al, 2017), Acari (Gotoh et al, 2007(Gotoh et al, , 2003Vala et al, 2002), Isopoda (Cordaux et al, 2012;Sicard et al, 2014), and Arachnids (Curry et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Symbiont-Mediated Cytoplasmic Incompatibility: What have we Learned in 50 Years?

Shropshire¹,

Leigh²,

Bordenstein³

2020

Preprint

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Cytoplasmic incompatibility (CI) is the most common symbiont-induced reproductive manipulation. Specifically, symbiont-induced sperm modifications cause catastrophic mitotic defects in the fertilized embryo and ensuing lethality in crosses between symbiotic males and either aposymbiotic females or females harboring a different symbiont strain. However, if the female carries the same symbiont strain, then embryos develop properly, which imparts a relative fitness benefit to symbiont-transmitting mothers. Thus, CI drives maternally transmitted bacteria to high frequencies in arthropod species worldwide. In the past two decades, CI has experienced a boom in interest due in part to its (i) deployment in successful, worldwide efforts to reduce the spread of mosquito-borne diseases, (ii) causation by bacteriophage genes, cifA and cifB, that modify animal reproductive processes, and (iii) important impacts on incipient speciation. This review serves as a gateway to experimental, conceptual, and quantitative themes of CI and outlines significant gaps in our understanding of CI’s mechanism that are ripe for investigation from a diversity of subdisciplines in the life sciences.

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Identification and characterization of bacterial symbionts in three species of filth fly parasitoids

Cited by 18 publications

References 45 publications

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Wolbachia influence on the fitness of Anagyrus vladimiri (Hymenoptera: Encyrtidae), a bio‐control agent of mealybugs

Symbiont-mediated cytoplasmic incompatibility: What have we learned in 50 years?

Symbiont-Mediated Cytoplasmic Incompatibility: What have we Learned in 50 Years?

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