Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed

Guo, Xia; Chen, Dandan; Kong, Peng; Cui, Zhengwei; Zhang, Xu-Jie; Li, Shun; Zhang, Yongan

doi:10.1016/j.fsi.2016.03.017

Cited by 110 publications

(65 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The acid and bile tolerances of KCCM 43270 spores were determined according to Niu et al () with slight modifications. The spores were produced according to the method of Guo et al (). About 1% (v/v) of overnight grown culture of KCCM 43270 was inoculated into sterile DSM medium (nutrient broth, 0.8% (w/v); KCl, 0.1% (w/v); MgSO 4 ∙7H 2 O, 0.025% (w/v); Ca(NO 3 ) 2 , 1 mmol/L; MnCl 2 , 10 μmol/L; FeSO 4 , 1 μmol/L) and incubated at 37°C and 200 rpm for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed

et al. 2018

View full text Add to dashboard Cite

In recent years, Bacillus spp. have garnered attention as probiotic supplements in aquafeed owing to the production of heat stable and low pH resistant spores. Herein, we isolated and characterized an autochthonous Bacillus licheniformis KCCM 43270 from the intestine of olive flounder ( Paralichthys olivaceus ) for supplementation in low‐fishmeal extruded aquafeeds. The KCCM 43270 was screened based on amylase, protease, cellulase, and lipase as well as non‐hemolytic activities. The isolate was able to grow in carboxymethyl cellulose (CMC), xylan, and soybean meal (SBM) when used as a single carbon source in the minimal nutrient M9 medium. The KCCM 43270 spores displayed complete survival in acid (pH 2.5) and bile (0.3%, w/v) for 3 hr, strong biofilm formation, and nearly 50% adhesion with intestinal mucus. The spores of the isolate also showed significant survival ability at 80, 90, 100°C for 60, 30, and 1 min, respectively. In addition, the spores in a blend of SBM complex carrier showed significant heat stability at 120°C for 5 min and under different drying conditions. Furthermore, the spores also survived the extrusion process during low‐fishmeal aquafeed manufacturing, implying the potential application of B. licheniformis KCCM 43270 in aquafeed industry.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The acid and bile tolerances of KCCM 43270 spores were determined according to Niu et al (2018) with slight modifications. The spores were produced according to the method of Guo et al (2016).…”

Section: Acid Bile and Heat Tolerances Of Kccm 43270 Sporesmentioning

confidence: 99%

Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Grass carp is herbivorous; thus, cellulase-producing bacteria are particularly important for food digestion, especially when fish are fed high-cellulose diets (Li et al, 2009;Liu et al, 2016;Wu, Wang et al, 2012). Interestingly, a positive correlation between the content of food fibre and diversity of cellulolytic bacteria in the grass carp intestine was reported as follows: grass carp fed high-fibre grass harboured a higher diversity of cellulolytic bacterial communities than the fish fed low-fibre feedstuff (Li et al, 2016). Recent researches based on either traditional or molecular approaches have supported this idea.…”

Section: Intestinal Microbiota Of Grass Carpmentioning

confidence: 95%

“…In grass carp, intestinal microbes are useful not only for digesting dietary polysaccharides indigestible to host, but also for achieving nutritional and physiological homeostasis (Ni, Yan, Yu & Zhang, 2014). Intestinal microbial composition is influenced by many factors, including host's species (Li, Yu, Feng, Yan & Gong, 2012), aquatic environment and geolocation (Li, Zhong et al, 2015), host's diet (Li et al, 2016;Wu, Wang et al, 2012;Zhou, Chen, Zhang & Chen, 1998) and host's dietary supplementation application Wang, Zhu et al, 2015;Wu, Jiang, Ling & Wang, 2015;Wu, Feng et al, 2012;Zhou et al, 2013). Regarding the host's diet, in an early work, Zhou et al (1998) The use of antimicrobials in disease control is relatively common in aquaculture industry; however, it also decreases the number of host's intestinal microbiota (Haygood & Jha, 2016;Ringø et al, 2016).…”

Section: Intestinal Bacterial Communities In Grass Carpmentioning

confidence: 99%

“…has been commonly used as an effective additive to the diet of grass carp (Li, Zhang et al, 2012;Li, et al, 2017;Shen et al, 2011;Zhang et al, 2013). A recent review paper, Guo et al (2016), has discussed the advantages of probiotic B. subtilis in grass carp in detail: spores of the bacterium are naturally produced, heat-stable and able to survive and germinate in the gut (at low pH); it forms biofilm and secretes antimicrobials; and finally, the bacterium is an attractive vehicle for delivery of heterologous antigens to intestinal tract. In another report, B. subtilis (at a concentration of 1.0 9 10 5 cfu/g feed) has been documented to promote growth performance, immunity and antioxidant ability of grass carp after a 45-day experimental period (Shen et al, 2011).…”

Section: Prebiotics and Synbiotics In Grass Carpmentioning

confidence: 99%

See 1 more Smart Citation

A review of intestinal microbes in grass carpCtenopharyngodon idellus(Valenciennes)

Tran

Wang

2017

Aquac Res

View full text Add to dashboard Cite

Grass carp, Ctenopharyngodon idellus, harbours complex intestinal bacterial communities, which are important in several physiological processes of their host. Intestinal microbiota of grass carp have been previously described in numerous studies. However, an overview on the bacterial community diversity, including their establishment, their functions in host's nutritional processes and immune‐related responses, and use as probiotics, is absent. This study aimed to summarize the current understanding of the grass carp intestinal microbiota. In this review, we provide general information on the establishment and composition of intestinal microbial communities and factors influencing the diversity of gut microbiota. Also, this review covers the dietary effects of probiotics, prebiotics and/or synbiotics on the grass carp intestinal microbial communities and physiological characteristics. Although our knowledge of the grass carp intestinal microbiota is expanding rapidly, further studies on the factors affecting the diversity of intestinal microbes, interactions between intestinal microbiota and their hosts and application of probiotics/prebiotics/synbiotics in aquaculture industry, are needed.

show abstract

Study of antagonism between some intestinal bacteria with high‐speed micellar electrokinetic chromatography

Wang

Zhang

et al. 2021

Electrophoresis

View full text Add to dashboard Cite

In this work, high‐speed micellar electrokinetic chromatography with LIF detection was applied to study the antagonism between three intestinal bacteria, Escherichia coli (E. coli), Bacillus licheniformis (B. licheniformis) and Bacillus subtilis (B. subtilis). The fluorescent derivatization for the bacteria was performed by labeling the bacteria with FITC. In a high‐speed capillary electrophoresis (HSCE) device, the three bacteria could be completely separated within 4 min under the separation mode MEKC. The BGE was 1 × TBE containing 30 mM SDS and 1.5 × 10–5 g/mL polyethylene oxide. The limits of detection for E. coli, B. licheniformis and B. subtilis were 2.80 × 106 CFU/mL, 1.60 × 106 CFU/mL and 1.90 × 106 CFU/mL respectively. Lastly, the method was applied to investigate the antagonism between the three bacteria. The bacteria were mixed and cultured for 7 days. The samples were separated and determined every day to study the interaction between bacteria. The results showed that B. licheniformis and B. subtilis could not inhibit each other, but they could effectively inhibit the reproduction of E. coli. The method developed in this work was quick, sensitive and convenient, and it had great potential in the application of antagonism study for bacteria.

show abstract

Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed

Cited by 110 publications

References 59 publications

Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed

Autochthonous Bacillus licheniformis: Probiotic potential and survival ability in low‐fishmeal extruded pellet aquafeed

A review of intestinal microbes in grass carpCtenopharyngodon idellus(Valenciennes)

Study of antagonism between some intestinal bacteria with high‐speed micellar electrokinetic chromatography

Contact Info

Product

Resources

About