Identification and characterization of an angiotensin-I converting enzyme inhibitory peptide from enzymatic hydrolysate of rape (Brassica napus L.) bee pollen

Zhu, Shuguang; Wang, Shan; Wang, Lingbo; Huang, Dejian; Chen, Shangwei

doi:10.1016/j.lwt.2021.111502

Cited by 14 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this work, the ratio of hydrophobic amino acids in LAKVAP was 83.33%, which was higher than that of HPVTGL (50.00%) identified from enzymatic hydrolysate of rape bee pollen. 9 This result confirmed that LP-6 identified in this work had a high ACE inhibitory effect in theory, which was consistent with our previous results of ACE inhibition.…”

Section: Identification Of Ace Inhibitorysupporting

confidence: 93%

Identification of a Novel Post-digestion Angiotensin I-Converting Enzyme Inhibitory Peptide from Silver Prussian Carp (Carassius auratus gibelio) Roe

Ling

Sheng

et al. 2023

ACS Food Sci. Technol.

View full text Add to dashboard Cite

Proteins of aquatic products are potential sources of bioactive peptides. This study attempted to screen 29 kinds of aquatic products with the highest angiotensin I-converting enzyme (ACE) inhibitory activity and to identify the active peptide. Results showed that the enzyme hydrolysate of silver Prussian carp roe exhibited the highest ACE inhibitory rate before (64.71%) and after (53.82%) digestion. LAKVAP identified from the enzymatic extract of silver Prussian carp roe was a competitive inhibitor, with an ACE inhibitory rate of 95.33%. The molecular docking study suggested that LAKVAP formed hydrogen bonds with S2 active sites (Gln281, His 353, and His513) and made coordinate bonds with Zn2+ (His383) of ACE, which contributed to the interaction between ACE and peptide. The results suggested that silver Prussian carp roe proteins could be suitable materials for ACE inhibitory peptide preparation, and LP-6 might be potentially beneficial as an antihypertensive agent.

show abstract

Section: Identification Of Ace Inhibitorysupporting

confidence: 93%

Identification of a Novel Post-digestion Angiotensin I-Converting Enzyme Inhibitory Peptide from Silver Prussian Carp (Carassius auratus gibelio) Roe

Ling

Sheng

et al. 2023

ACS Food Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Bee-pollen protein hydrolysates from Alcalase ® , Neutrase ® , and Flavourzyme ® , showed selectively antiproliferative effects against lung cancer cells, and were confirmed as a rich source of bioactive peptides [ 90 ]. A novel angiotensin-I-converting enzyme (ACE) inhibitory peptide was identified from rape bee-pollen protein hydrolysate using alcalase [ 91 ]. Three proteases involved in digestion, including pepsin, trysin, and papain, were applied to bee bread and enzymatic hydrolysates were obtained with antioxidant activity and ACE activities [ 92 ].…”

Section: Processing and Extraction Methods Of Bp Bioactivesmentioning

confidence: 99%

Bee Pollen and Bread as a Super-Food: A Comparative Review of Their Metabolome Composition and Quality Assessment in the Context of Best Recovery Conditions

et al. 2023

View full text Add to dashboard Cite

Recently, functional foods have been a subject of great interest in dietetics owing not only to their nutritional value but rather their myriad of health benefits. Moreover, an increase in consumers’ demands for such valuable foods warrants the development in not only production but rather tools of quality and nutrient assessment. Bee products, viz., pollen (BP) and bread, are normally harvested from the flowering plants with the aid of bees. BP is further subjected to a fermentation process in bee hives to produce the more valuable and bioavailable BB. Owing to their nutritional and medicinal properties, bee products are considered as an important food supplements rich in macro-, micro-, and phytonutrients. Bee products are rich in carbohydrates, amino acids, vitamins, fatty acids, and minerals in addition to a myriad of phytonutrients such as phenolic compounds, anthocyanins, volatiles, and carotenoids. Moreover, unsaturated fatty acids (USFAs) of improved lipid profile such as linoleic, linolenic, and oleic were identified in BP and BB. This work aims to present a holistic overview of BP and BB in the context of their composition and analysis, and to highlight optimized extraction techniques to maximize their value and future applications in nutraceuticals.

show abstract

“…C4:0 Butanoic acid; C6:0 Hexanoic acid; C8:0 Octanoic acid; C10:0 Decanoic acid; C11:0 Undecanoic acid; C12:0 Dodecanoic acid; C13:0 Tridecanoic acid; C14:0 Tetradecanoic acid; C14:1 (cis-9) (Z)-tetradec-9-enoic acid; C15:0 Pentadecanoic acid; C15:1 (cis-10) (Z)-pentadec-10-enoic; C16:0 Hexadecanoic acid; C16:1 (9Z)-hexadec-9-enoic acid; C17:0 Heptadecanoic acid; C17:1 cis-10-heptadecenoic; C18:0 Octadecanoic acid; C18:1 (E)-octadec-9-enoic; C18:1 (Z)-octadec-9-enoic; C18:2 (all-trans-9,12) Octadeca-9,12-dienoic acid; C18:2 (all-cis-9,12) (9Z,12Z)-octadeca-9,12-dienoic acid; C18:3 (all-cis-9,12,15) Octadeca-6,9,15-trienoic acid C20:0 Icosanoic acid; C18:3 (all-cis-6,9,12) Octadeca-6,9,12-trienoic acid;C20:1 (cis-11) (Z)-icos-11-enoic acid;; C20:2 (all-cis-11,14,) Icosa-11,14-dienoic acid; C20: GC-FID Beeswax (189) C12:0, C13:0, C15:0, C16:0, C18:0, C20:0, C24:0, C25, C16:1, C20, C27, C28, C29, C30, C31, C32, C33, C36; C14:0, C26, C18: Proteins, Peptides, and Amino Acids Bee products such as BCP, BB, and RJ are good sources of protein, which can be consumed as a food supplement to the diet. According to the characteristics of the protein, the extraction solvent usually focuses on distilled water (195), acid extraction (196,197), phosphate buffer with different pH (pH 7.6) (198), (pH 7.2) (199), or 50 mM Tris-HCl, pH 8 (200). The method for the protein extraction and determination are shown in Table 4.…”

Section: Compoundsmentioning

confidence: 99%

“…Bee products such as BCP, BB, and RJ are good sources of protein, which can be consumed as a food supplement to the diet. According to the characteristics of the protein, the extraction solvent usually focuses on distilled water ( 195 ), acid extraction ( 196 , 197 ), phosphate buffer with different pH (pH 7.6) ( 198 ), (pH 7.2) ( 199 ), or 50 mM Tris-HCl, pH 8 ( 200 ). The method for the protein extraction and determination are shown in Table 4 .…”

Section: Processing Techniques Applied In Bee Productsmentioning

confidence: 99%

Processing Technologies for Bee Products: An Overview of Recent Developments and Perspectives

Luo

Dong

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

Increased demand for a more balanced, healthy, and safe diet has accelerated studies on natural bee products (including honey, bee bread, bee collected pollen royal jelly, propolis, beeswax, and bee venom) over the past decade. Advanced food processing techniques, such as ultrasonication and microwave and infrared (IR) irradiation, either has gained popularity as alternatives or combined with conventional processing techniques for diverse applications in apiculture products at laboratory or industrial scale. The processing techniques used for each bee products have comprehensively summarized in this review, including drying (traditional drying, infrared drying, microwave-assisted traditional drying or vacuum drying, and low temperature high velocity-assisted fluidized bed drying), storage, extraction, isolation, and identification; the assessment methods related to the quality control of bee products are also fully mentioned. The different processing techniques applied in bee products aim to provide more healthy active ingredients largely and effectively. Furthermore, improved the product quality with a shorter processing time and reduced operational cost are achieved using conventional or emerging processing techniques. This review will increase the positive ratings of the combined new processing techniques according to the needs of the bee products. The importance of the models for process optimization on a large scale is also emphasized in the future.

show abstract

Identification and characterization of an angiotensin-I converting enzyme inhibitory peptide from enzymatic hydrolysate of rape (Brassica napus L.) bee pollen

Cited by 14 publications

References 29 publications

Identification of a Novel Post-digestion Angiotensin I-Converting Enzyme Inhibitory Peptide from Silver Prussian Carp (Carassius auratus gibelio) Roe

Identification of a Novel Post-digestion Angiotensin I-Converting Enzyme Inhibitory Peptide from Silver Prussian Carp (Carassius auratus gibelio) Roe

Bee Pollen and Bread as a Super-Food: A Comparative Review of Their Metabolome Composition and Quality Assessment in the Context of Best Recovery Conditions

Processing Technologies for Bee Products: An Overview of Recent Developments and Perspectives

Contact Info

Product

Resources

About