Identification and characterization of ABCB1-mediated and non-apoptotic sebum secretion in differentiated hamster sebocytes

Kurihara, Hirokazu; Sato, Takashi; Akimoto, Noriko; Ogura, Takayuki; Ito, Akira

doi:10.1016/j.bbalip.2011.08.011

Cited by 8 publications

(16 citation statements)

References 34 publications

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“…Since sebum secretion is generally considered to be regulated by a holocrine mechanism, which may involve the apoptosis of sebocytes (27), it was confirmed that there were no apoptotic cells in this experimental condition (data not shown). In addition, the current authors have previously reported on the apoptosis-independent and ATP-binding cassette (ABC) transporter (ABCB1/P-glycoprotein) -mediated TG secretion in DHS (28). Regarding the relationship between ABCB1 activity and UV irradiation, Trindade et al (29) have reported that ABCB1-overexpressing leukemia cells with a multidrug-resistance phenotype are resistant to UVA but sensitive to UVB and UVC.…”

Section: Discussionmentioning

confidence: 99%

Triptolide suppresses ultraviolet B-enhanced sebum production by inhibiting the biosynthesis of triacylglycerol in hamster sebaceous glands in vivo and in vitro

Sato

Akimoto

Takahashi

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Ultraviolet B (UVB) irradiation causes alterations in cutaneous barrier function, including excessive production of sebum in sebaceous glands, which is associated with the aggravation of acne. This study aimed to evaluate the inhibitory effects of triptolide, a diterpenoid triepoxide from Tripterygium wilfordii Hook F, on sebocytic lipogenesis in UVB-irradiated hamster skin in vivo and in vitro. Topical application of triptolide decreased the UVB-enhanced sebum accumulation in the sebaceous glands of hamster skin. The level of triacylglycerol (TG), a major sebum component, on the skin surface was reduced by triptolide treatment in UVB-irradiated hamsters, whereas there was no change in that of free-fatty acids and cholesterol, which are minor sebum components. UVB irradiation significantly enhanced TG production (P<0.01 in extracellular lipids, P<0.05 in intracellular lipids), and the activity of acyl coenzyme A/diacylglycerol acyltransferase (DGAT), a rate-limiting enzyme of TG synthesis, in differentiated hamster sebocytes (P<0.05 at 6 h and UVB of 0.62 kJ/m 2 , P<0.001 at 24 h and UVB 0.37 or 0.62 kJ/m 2 ). Furthermore, triptolide significantly inhibited UVB-enhanced TG production (P<0.05 at 28 nM and P<0.01 at 56 and 112 nM triptolide) and DGAT activity (P<0.01 at 28 nM and P<0.001 at 56 and 112 nM triptolide) in differentiated hamster sebocytes. These results provide novel evidence that triptolide decreases UVB-enhanced sebum production by inhibiting DGAT-dependent TG biosynthesis in differentiated hamster sebocytes. These findings may be applicable to the prevention of acne aggravation.

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Section: Discussionmentioning

confidence: 99%

Triptolide suppresses ultraviolet B-enhanced sebum production by inhibiting the biosynthesis of triacylglycerol in hamster sebaceous glands in vivo and in vitro

Sato

Akimoto

Takahashi

et al. 2017

Experimental and Therapeutic Medicine

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“…These are lipid‐laden and feature pyknotic nuclei before cell rupture and dispersion of the cell contents, completing the process of holocrine secretion , a unique method of secretion employed by the SG . The mechanisms of holocrine secretion are still hypothetical, but include the lysis of sebocytes by hydrolytic enzymes within lysosomes , apoptosis‐induced cell lysis and/or the release of sebum via an ABCB1 transporter‐dependent mechanism .…”

Section: Sebocytesmentioning

confidence: 99%

“…Therefore, we also encourage the standardisation of histomorphometric techniques to evaluate changes in SG size/activities, thus hoping to improve the inter‐observer reproducibility of published histological data, for example in trials of SG disorders . The techniques described here are transferable to isolated/specialised SGs and are relevant to other SG research models, for example hamster SGs and cultured sebocytes .…”

Section: Introductionmentioning

confidence: 97%

“…The SG is one of the most intriguing mammalian skin structures as it can serve as a model for studying numerous central life sciences issues, for example in cell biology and in skin, lipid or hormone research . Moreover, the SG is unique in at least two ways: its main cellular component, sebocytes, is of epithelial origin, yet engage in lipid production and metabolism, a feature usually associated with adipocytes; and sebum is produced by holocrine secretion, that is, via an unusual mode of cell suicide that sebocytes undergo .…”

Section: Introductionmentioning

confidence: 99%

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A practical guide for the study of human and murine sebaceous glands in situ

Hinde

Haslam

Schneider³

et al. 2013

Experimental Dermatology

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The skin of most mammals is characterised by the presence of sebaceous glands (SGs), whose predominant constituent cell population is sebocytes, that is, lipid-producing epithelial cells, which develop from the hair follicle. Besides holocrine sebum production (which contributes 90% of skin surface lipids), multiple additional SG functions have emerged. These range from antimicrobial peptide production and immunomodulation, via lipid and hormone synthesis/metabolism, to the provision of an epithelial progenitor cell reservoir. Therefore, in addition to its involvement in common skin diseases (e.g. acne vulgaris), the unfolding diversity of SG functions, both in skin health and disease, has raised interest in this integral component of the pilosebaceous unit. This practical guide provides an introduction to SG biology and to relevant SG histochemical and immunohistochemical techniques, with emphasis placed on in situ evaluation methods that can be easily employed. We propose a range of simple, established markers, which are particularly instructive when addressing specific SG research questions in the two most commonly investigated species in SG research, humans and mice. To facilitate the development of reproducible analysis techniques for the in situ evaluation of SGs, this methods review concludes by suggesting quantitative (immuno-)histomorphometric methods for standardised SG evaluation.

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“…Sebum secretion is generally considered to be regulated by a holocrine mechanism, which may contain sebocyte apoptosis . We have recently reported that secretion of triacylglycerol (TG), a major sebum component, is augmented in hamster sebocytes in cell differentiation‐dependent and apoptosis‐independent manners . Therefore, not only a holocrine mechanism but also apoptosis‐independent pathways may be involved in sebum secretion in sebaceous glands.…”

Section: Introductionmentioning

confidence: 99%

Differentiated hamster sebocytes exhibit apoptosis‐resistant phenotype by the augmentation of intracellular calcium level in vitro

Kurihara

Sato

Akimoto

et al. 2012

Experimental Dermatology

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Sebaceous glands play important roles in the maintenance of the skin barrier function by secreting sebum onto the skin surface. In our study, we demonstrated that differentiated hamster sebocytes (DHS) exhibited apoptosis resistance and the loss of Ca 2+ influx against a calcium ionophore, A23187 treatment, which induced both apoptosis and Ca 2+ influx in undifferentiated hamster sebocytes (unDHS). The Fluo-3-related signal of intracellular Ca 2+ in the DHS was higher than that in unDHS and was sustained even though there was a depletion of Ca 2+ from the culture medium.Furthermore, the intracellular Ca 2+ chelator, BAPTA-AM, was found to decrease the Ca 2+ signal in the DHS, which induced apoptosis. Thus, these results provide novel evidence that the cell differentiation-dependent increase in store-operated Ca 2+ release is associated with apoptosis resistance in the DHS. Moreover, these findings should accelerate the understanding of the mechanisms of sebogenesis and/or sebum production and secretion under physiological conditions.Abbreviations: TG, triacylglycerol; DHS, differentiated hamster sebocytes; unDHS, undifferentiated hamster sebocytes; BAPTA-AM, 1,2-bis (2-aminophenoxy) ethane-N,N,N′,N′-tetraacetic acid tetrakis acetoxymethyl ester; FITC, fluorescein isothiocyanate; PI, propidium iodide; PS phosphatidylserine; 2-APB, 2-aminoethyl diphenylborinate.

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Identification and characterization of ABCB1-mediated and non-apoptotic sebum secretion in differentiated hamster sebocytes

Cited by 8 publications

References 34 publications

Triptolide suppresses ultraviolet B-enhanced sebum production by inhibiting the biosynthesis of triacylglycerol in hamster sebaceous glands in vivo and in vitro

Triptolide suppresses ultraviolet B-enhanced sebum production by inhibiting the biosynthesis of triacylglycerol in hamster sebaceous glands in vivo and in vitro

A practical guide for the study of human and murine sebaceous glands in situ

Differentiated hamster sebocytes exhibit apoptosis‐resistant phenotype by the augmentation of intracellular calcium level in vitro

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