Identification and Characterization of a Gene Encoding an ABC Transporter Expressed in the Dicarboxylic Acid-Producing Yeast<i>Candida maltosa</i>

Sagehashi, Yoshiyuki; Horiuchi, Hiroyuki; Fukuda, Ryouichi; Ohta, Akio

doi:10.1271/bbb.130595

Cited by 3 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, time course qualitative analysis of free fatty acids can also provide further insight into their threshold for the induction of background ω-oxidation activity. Despite the good LCDCA transport efficiency of our platform, the overexpression of potential heterologous transporters such as CmCDR1 [127], or homologous transporter like ABC2 [128] could improve LCDCA export to minimize product inhibition. Optimization of culture conditions was beyond the scope of this study.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Engineering of Yarrowia lipolytica as a Promising Cell Biorefinery Platform for the de novo Production of Multi-Purpose Long Chain Dicarboxylic Acids

2017

View full text Add to dashboard Cite

This proof-of-concept study establishes Yarrowia lipolytica (Y. lipolytica) as a whole cell factory for the de novo production of long chain dicarboxylic acid (LCDCA-16 and 18) using glycerol as the sole source of carbon. Modification of the fatty acid metabolism pathway enabled creating a pool of fatty acids in a β-oxidation deficient strain. We then selectively upregulated the native fatty acid ω-oxidation pathway for the enhanced terminal oxidation of the endogenous fatty acid precursors. Nitrogen-limiting conditions and leucine supplementation were employed to induce fatty acid biosynthesis in an engineered Leu -modified strain. Our genetic engineering strategy allowed a minimum production of 330 mg/L LCDCAs in shake flask. Scale up to a 1-L bioreactor increased the titer to 3.49 g/L. Our engineered yeast also produced citric acid as a major by-product at a titer of 39.2 g/L. These results provide basis for developing Y. lipolytica as a safe biorefinery platform for the sustainable production of high-value LCDCAs from non-oily feedstock.

show abstract

Section: Discussionmentioning

confidence: 99%