Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI)

GTP-binding proteins are found in all organisms. They are important switches that cycle between an active and an inactive state, ensuring vectorial flow of information on the expense of guanosine triphosphate (GTP). In this review, we discuss current progress in the molecular characterization and functional analysis of plant genes encoding heterotrimeric and small GTPases. An up-to-date list including all cloned plant GTPase genes is given and a systematic classification is proposed.

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“…Plant GTPases 246 expression pattern was also shown for VcRabGDI protein, which is the only RabGDI protein in Vol6ox [179].…”

Section: Proteins Interacting With Rab Gtpasesmentioning

confidence: 78%

GTP-binding proteins in plants

Bischoff¹,

Molendijk²,

Rajendrakumar³

et al. 1999

CMLS, Cell. Mol. Life Sci.

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“…The homology to other Rab-GDIs ranged from 49% to 63%. The closest relative is the recently isolated Rab-GDI from Volvox carterii with 63% identity [30]. To explore further the similarity of AtGDIl with other members of the GDI family we used an algorithm which finds statistically significant blocks of continuous amino acids shared between multiple protein sequences.…”

Section: Discussionmentioning

confidence: 99%

At‐GDI1fromArabidopsis thalianaencodes a rab‐specific GDP dissociation inhibitor that complements thesec19mutation ofSaccharomyces cerevisiae

Žárský¹,

Cvrčková²,

Bischoff³

et al. 1997

FEBS Letters

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Rab GTPases play a central role in the control of vesicular membrane traffic. These proteins cycle between cytosolic and membrane-bound compartments in a guanine nucleotide-dependent manner, a process that is regulated by several accessory proteins. Of particular interest are the Rab guanosine nucleotide diphosphate dissociation inhibitor proteins (Rab-GDI) which bind to prenylated Rab GTPases, slow the rate of GDP dissociation and escort GDP bound Rab proteins to their target membranes and retrieve them after completion of their catalytic cycle. We have cloned from Arabidopsis thaliana a cDNA coding for the Rab guanosine diphosphate dissociation inhibitor (AtGDIl) by functional complementation of the Saccharomyces cerevisiae seel9-1 mutant. The Arabidopsis cDNA potentially encodes a 49850 Da protein which is homologous to yeast GDI (49%) and to other members of the Rab-GDI family (49-63%). Northern blot analysis indicates that the mRNA is expressed in all tissues examined. The existence of a plant homologue of the Rab-GDI family indicates that the basic vesicle traffic control machinery may be highly conserved in plants as it is in yeast and mammals.

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“…Finally a large subgroup of Ypt/Rab proteins related to mammalian Rablls are also present in plant cells (Borg et al 1997). Since homologues for GDI (Beyser and Fabry 1996) and GAP(GTPase-activating protein)-like activity (Anai et al 1994) have been also identified in plants, it is a safe assumption that the Rab cycle functions in the same way as it does in mammalian and yeast cells, and that membrane dynamics in plants is also under the control of Rab proteins. Whether each of these Rabs has the same subcellular distribution as in mammalian cells and plays (a) similar role(s) in plant cells remains to be established, and certainly an effort should be directed towards this direction to study vesicle transport in plant cells.…”

Section: Rab Proteinsmentioning

confidence: 96%

Membrane transport in the endocytic pathway: Animal versus plant cells

Marcote

Grüenberg

et al. 2000

Protoplasma

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Summary.The endocytic pathway is a well established process in animal cells, but it is not well understood in plant cells. At the morphological level, all the compartments involved in endocytosis in animal cells seem to have counterparts in plant cells, and the organization of the pathway appears to share some striking similarities. Several Rab homologues have been found in plant cells, including homologues of Rab5, Rab7, and Rabll, markers of endocytic compartments in animal cells. Coat proteins are also present in plant cells, including clathrin, adaptins, and ADP ribosylation factor proteins. However, endocytic compartments in plant cells also exhibit specific features both in organization and function. The molecular composition of these compartments remains to be established, and future work will be necessary to identify the key regulators of endocytic trafficking in plant cells.

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Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI)

Cited by 12 publications

References 47 publications

GTP-binding proteins in plants

GTP-binding proteins in plants

At‐GDI1fromArabidopsis thalianaencodes a rab‐specific GDP dissociation inhibitor that complements thesec19mutation ofSaccharomyces cerevisiae

Membrane transport in the endocytic pathway: Animal versus plant cells

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