Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli

Petrin, Sara; Orsini, Massimiliano; Mastrorilli, Eleonora; Longo, Alessandra; Cozza, D.; Olsen, John Elmerdahl; Ricci, Antonia; Losasso, Carmen; Barco, Lisa

doi:10.1016/j.plasmid.2021.102566

Cited by 4 publications

(2 citation statements)

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“…Besides horizontal transfer, also translocation from plasmids to chromosome has been described, creating clusters or antimicrobial resistance islands that are now regarded as an efficient means of resistance genes dissemination ( 67 , 93 , 94 ). Moreover, MGEs together with integrons, transposons and insertion sequences, favoring genetic recombination mechanisms, facilitate the accumulation on resistance islands ( 70 , 95 , 96 ). Another explanation could be that multiple resistance genes are carried on very large plasmids, as in the case of the pESI megaplasmid in S. infantis ( 42 , 97 ).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in Salmonella spp. isolates from Italy reveal high heterogeneity among serovars

Petrin,

Orsini,

Massaro

et al. 2023

Front. Public Health

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IntroductionThe spread of antimicrobial resistance among zoonotic pathogens such as Salmonella is a serious health threat, and mobile genetic elements (MGEs) carrying antimicrobial resistance genes favor this phenomenon. In this work, phenotypic antimicrobial resistance to commonly used antimicrobials was studied, and the antimicrobial resistance genes (ARGs) and plasmid replicons associated with the resistances were determined.MethodsEighty-eight Italian Salmonella enterica strains (n = 88), from human, animal and food sources, isolated between 2009 and 2019, were selected to represent serovars with different frequency of isolation in human cases of salmonellosis. The presence of plasmid replicons was also investigated.Results and discussionResistances to sulphonamides (23.9%), ciprofloxacin (27.3%), ampicillin (29.5%), and tetracycline (32.9%) were the most found phenotypes. ARGs identified in the genomes correlated with the phenotypical results, with blaTEM-1B, sul1, sul2, tetA and tetB genes being frequently identified. Point mutations in gyrA and parC genes were also detected, in addition to many different aminoglycoside-modifying genes, which, however, did not cause phenotypic resistance to aminoglycosides. Many genomes presented plasmid replicons, however, only a limited number of ARGs were predicted to be located on the contigs carrying these replicons. As an expectation of this, multiple ARGs were identified on contigs with IncQ1 plasmid replicon in strains belonging to the monophasic variant of Salmonella Typhimurium. In general, high variability in ARGs and plasmid replicons content was observed among isolates, highlighting a high level of heterogeneity in Salmonella enterica. Irrespective of the serovar., many of the ARGs, especially those associated with critically and highly important antimicrobials for human medicine were located together with plasmid replicons, thus favoring their successful dissemination.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in Salmonella spp. isolates from Italy reveal high heterogeneity among serovars

Petrin,

Orsini,

Massaro

et al. 2023

Front. Public Health

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“…The I-complex plasmids contain incompatibility groups I, K, B, and Z. IncI, or MOBP according to relaxase typing, is a group of low copy-number, narrow-host-range, conjugative plasmids, which vary in size from 50 to 250 kb (2,3). Some indistinguishable IncI1 and IncIplasmids have been isolated from multiple, unrelated high-risk clones, identified in different periods and bacterial species, in distant geographical areas, defined as "epidemic" plasmids (4)(5)(6)(7). IncI pST3 plasmid is the most commonly reported blaCTX-M-1-IncI1 plasmid in Europe, described in pathogenic human E. coli isolates, and in E. coli, and Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Dissemination of IncI plasmid encodingbla_CTX-M-1is not hampered by its fitness cost in the pig’s gut

Allain

Mahérault

Gachet

et al. 2023

Preprint

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Multiresistance plasmids belonging to the IncI incompatibility group have become one of the most pervasive plasmid types in extended-spectrum beta-lactamase producingEscherichia coliof animal origin. The extent of the burden imposed on the bacterial cell by these plasmids seems to contribute to the emergence of "epidemic" plasmids. However,in vivodata in the natural environment of the strain are scarce. Here, we investigated the cost of ablaCTX-M-1-IncI1 epidemic plasmid in a commensalE. colianimal strain, UB12-RC, before and after oral inoculation of fifteen 6- to 8-week-old specific pathogen-free pigs. Growth rate in rich medium was determined on (i) UB12-RC and derivatives, with or without plasmid,in vivoand/orin vitroevolved, and (ii) strains that acquired the plasmid in the gut during the experiment. AlthoughblaCTX-M-1-IncI1 plasmid imposed no measurable burden on the recipient strain after conjugation and during the longitudinal carriage in the pig's gut, we observed a significant difference in the bacterial growth rate between IncI1 plasmid-carrying and plasmid-free isolates collected duringin vivocarriage. Only a few mutations on the chromosome of the UB12-RC derivatives were detected by whole-genome sequencing. RNA-Seq analysis of a selected set of these strains showed that transcriptional responses to theblaCTX-M-1-IncI1 acquisition were limited, affecting metabolism, stress response, and motility functions. Our data suggest that the effect of IncI plasmid on host cells is limited, fitness cost being insufficient to act as a barrier to IncI plasmid spread among natural population ofE. coliin the gut niche.

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Nationwide surveillance and characterization of the third-generation cephalosporin-resistant Salmonella enterica serovar infantis isolated from chickens in South Korea between 2010 and 2022

Kang,

Ali,

et al. 2024

Heliyon

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Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli

Cited by 4 publications

References 51 publications

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in Salmonella spp. isolates from Italy reveal high heterogeneity among serovars

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in Salmonella spp. isolates from Italy reveal high heterogeneity among serovars

Dissemination of IncI plasmid encodingbla_CTX-M-1is not hampered by its fitness cost in the pig’s gut

Nationwide surveillance and characterization of the third-generation cephalosporin-resistant Salmonella enterica serovar infantis isolated from chickens in South Korea between 2010 and 2022

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Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli

Cited by 4 publications

References 51 publications

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in Salmonella spp. isolates from Italy reveal high heterogeneity among serovars

Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization in Salmonella spp. isolates from Italy reveal high heterogeneity among serovars

Dissemination of IncI plasmid encodingblaCTX-M-1is not hampered by its fitness cost in the pig’s gut

Nationwide surveillance and characterization of the third-generation cephalosporin-resistant Salmonella enterica serovar infantis isolated from chickens in South Korea between 2010 and 2022

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Dissemination of IncI plasmid encodingbla_CTX-M-1is not hampered by its fitness cost in the pig’s gut