Identification and characterization of a major building block in the cell wall of <i>Saccharomyces cerevisiae</i>

Klis, Frans M.; P., L. H.; Vossen, Jack H.; Kapteyn, J.C.; Ram, Arthur F. J.; Montijn, R.C.; Berkel, M.A.A. van; Ende, H. van den

doi:10.1042/bst0250856

Cited by 37 publications

(20 citation statements)

References 12 publications

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“…Our existing knowledge cannot explain such high numbers of mannose residues in alkali‐extracted glucan molecules unless it is assumed that, before alkali extraction, each glucan molecule was attached to multiple GPI–CWPs, each with its own tetramannoside derived from the GPI remnant (note that alkali extraction cleaves the phosphodiester bridge in the GPI remnant, leaving the tetramannoside of the GPI remnant linked to 1,6‐β‐glucan). This seems unlikely in view of the data by Katohda et al 75, as recalculated by Klis et al 81, which indicate that the number of wall proteins is comparable to the number of 1,3‐β‐glucan molecules in the wall.…”

Section: Molecular Structure Of the Cell Wallmentioning

confidence: 91%

Cell wall construction in Saccharomyces cerevisiae

Klis¹,

Boorsma²,

Groot³

2006

Yeast

655

475

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In this review, we discuss new insights in cell wall architecture and cell wall construction in the ascomycetous yeast Saccharomyces cerevisiae. Transcriptional profiling studies combined with biochemical work have provided ample evidence that the cell wall is a highly adaptable organelle. In particular, the protein population that is anchored to the stress-bearing polysaccharides of the cell wall, and forms the interface with the outside world, is highly diverse. This diversity is believed to play an important role in adaptation of the cell to environmental conditions, in growth mode and in survival. Cell wall construction is tightly controlled and strictly coordinated with progression of the cell cycle. This is reflected in the usage of specific cell wall proteins during consecutive phases of the cell cycle and in the recent discovery of a cell wall integrity checkpoint. When the cell is challenged with stress conditions that affect the cell wall, a specific transcriptional response is observed that includes the general stress response, the cell wall integrity pathway and the calcineurin pathway. This salvage mechanism includes increased expression of putative cell wall assemblases and some potential cross-linking cell wall proteins, and crucial changes in cell wall architecture. We discuss some more enzymes involved in cell wall construction and also potential inhibitors of these enzymes. Finally, we use both biochemical and genomic data to infer that the architectural principles used by S. cerevisiae to build its cell wall are also used by many other ascomycetous yeasts and also by some mycelial ascomycetous fungi.

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Section: Molecular Structure Of the Cell Wallmentioning

confidence: 91%

Cell wall construction in Saccharomyces cerevisiae

Klis¹,

Boorsma²,

Groot³

2006

Yeast

655

475

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“…Each module has ␤-1,6-glucan interconnects chitin and mannoprotein to ␤-1,3-d-glucan [52,54]. Assembly of modules forms the yeast cell wall [55].…”

Section: Fungal Polysaccharide Brmsmentioning

confidence: 99%

Polysaccharide biological response modifiers

et al. 2006

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“…Cell wall architecture has been studied most extensively in S. cerevisiae and is likely to be a model for C. albicans since there are some similar observations, in particular sensitivity to enzymatic digestion, glucan-mannoprotein linkages, and candidate proteins, that fit the same model (237,238,246,247,268,462,463). In a very recent study, Kollár et al (268) detected the presence of material containing all four major cell wall components, ␤-1,3-glucan, ␤-1,6-glucan, chitin, and mannoprotein.…”

Section: Organizationmentioning

confidence: 99%

“…The proportion of cell wall polysaccharide involved in this type of structure is not clear. The following cell wall building block, where the linkages are indicated by the long dashes, is proposed (247,268): Mannoprotein-GPI remnant-␤-1,6-glucan-␤-1,3-glucan-chitin. The authors point out that these linkages are likely to be formed in the periplasmic space as a common end of the individual biosynthetic pathways.…”

Section: Organizationmentioning

confidence: 99%

Cell Wall and Secreted Proteins ofCandida albicans: Identification, Function, and Expression

Chaffin

López-Ribot

Casanova

et al. 1998

Microbiol Mol Biol Rev

655

344

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SUMMARY The cell wall is essential to nearly every aspect of the biology and pathogenicity of Candida albicans. Although it was intially considered an almost inert cellular structure that protected the protoplast against osmotic offense, more recent studies have demonstrated that it is a dynamic organelle. The major components of the cell wall are glucan and chitin, which are associated with structural rigidity, and mannoproteins. The protein component, including both mannoprotein and nonmannoproteins, comprises some 40 or more moieties. Wall proteins may differ in their expression, secretion, or topological location within the wall structure. Proteins may be modified by glycosylation (primarily addition of mannose residues), phosphorylation, and ubiquitination. Among the secreted enzymes are those that are postulated to have substrates within the cell wall and those that find substrates in the extracellular environment. Cell wall proteins have been implicated in adhesion to host tissues and ligands. Fibrinogen, complement fragments, and several extracellular matrix components are among the host proteins bound by cell wall proteins. Proteins related to the hsp70 and hsp90 families of conserved stress proteins and some glycolytic enzyme proteins are also found in the cell wall, apparently as bona fide components. In addition, the expression of some proteins is associated with the morphological growth form of the fungus and may play a role in morphogenesis. Finally, surface mannoproteins are strong immunogens that trigger and modulate the host immune response during candidiasis.

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Identification and characterization of a major building block in the cell wall of Saccharomyces cerevisiae

Abstract: Genetics of LPS biosynthesis Until recently, the genes responsible for LPS biosynthesis were only known in enteric bacteria, specifically Escherichia coli and Salmonella spp. Volume 25

Cited by 37 publications

References 12 publications

Cell wall construction in Saccharomyces cerevisiae

Cell wall construction in Saccharomyces cerevisiae

Polysaccharide biological response modifiers

Cell Wall and Secreted Proteins ofCandida albicans: Identification, Function, and Expression

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