Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis

Knight, Angus; Ni, Haolin; Cartwright, Keith; McFadden, Johnjoe

doi:10.1111/j.1365-2958.1992.tb00878.x

Cited by 38 publications

(34 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The only other IS characterized in N. meningitidis so far, IS1106, has been placed in the same group (22,32). IS4 IS are found in very heterogeneous bacterial sources, and thus distribution by horizontal gene transfer has been proposed.…”

Section: Discussionmentioning

confidence: 99%

Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains

et al. 1996

View full text Add to dashboard Cite

The insertion element IS1301 has been shown to mediate capsule phase variation in Neisseria meningitidis serogroup B by reversible insertional inactivation of the siaA gene. We have determined the target site specificity of this element by cloning and sequencing the insertion sites of 12 identical IS1301 copies found in N. meningitidis B1940. A target consensus core of 5-AYTAG-3 was identified, with the central TA being duplicated following insertion. Additional features around the target sites, including extended palindromic symmetry, stem-loop formation, and the high incidence of AT tracts, indicate that other factors, such as DNA secondary structure, are involved in target recognition. The left inverted repeat of an IS1016-like element acts as a hot spot for insertion, with one insertion element combination located upstream of the frpC gene. According to further sequence analysis, we were able to place IS1301 in the IS5 subgroup within the IS4 family of elements. A survey of 135 Neisseria strains indicated the presence of IS1301 in 27.9 to 33.3% of N. meningitidis serogroup B, C, and W135 strains and in 86.7% of serogroup Y strains. IS1301 did not occur in serogroup A strains, in Neisseria gonorrhoeae, or in apathogenic Neisseria spp.

show abstract

Section: Discussionmentioning

confidence: 99%

Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains

et al. 1996

View full text Add to dashboard Cite

show abstract

“…IS1248 from P. denitrificans closely resembles IS869, which resides on the Ti plasmid of Agrobacterium tumefaciens (18). Other members belonging to this group of IS elements include IS427, also found in A. tumefaciens (7); IS402 from Pseudomonas cepacia (10); ISmyco, found in Mycobacterium tuberculosis (16); IS1106 from Neisseria meningitidis (13); Tn4811 from Streptomyces lividans (4); ISRm4 of Rhizobium meliloti (23); another previously identified IS found in R. meliloti (19); and IS1031 of Acetobacter xylinum (6). ISmyco, the IS element from R. meliloti, and IS1031 have only single ORFs.…”

mentioning

confidence: 99%

Integration of heterologous DNA into the genome of Paracoccus denitrificans is mediated by a family of IS1248-related elements and a second type of integrative recombination event

1995

View full text Add to dashboard Cite

All members of the IS1248 family residing in the genome of Paracoccus denitrificans have been isolated by using a set of insertion sequence entrapment vectors. The family consists of five closely related members that integrate the entrapment vectors at distinct sites. One of these, IS1248b, was sequenced and, except for a single base change, shown to be identical to the previously isolated IS1248a. Southern analysis of genomic DNA with labeled IS1248 revealed different hybridization patterns for different isolates of P. denitrificans and Thiosphaera pantotropha. No hybridization was observed with DNA from Thiobacillus versutus and more distantly related species. From a comparison of the fingerprints it was shown that one of the members of the IS1248 family found in P. denitrificans DSM413 is absent in strain NCIB8944, although they are catalogued in international strain catalogues as identical strains. Furthermore, strains Pd1222 and Pd1235, both derivatives of P. denitrificans DSM413, were shown to have different patterns of IS1248 hybridizing restriction fragments. In 14 of 18 strains, the entrapment vectors used in this study were incorporated into the genome via IS1248-mediated cointegrate formation. In the other four strains, the entrapment vectors were shown to be integrated through a different mechanism not involving IS1248.

show abstract

“…), the method was less discriminatory than PFGE or MLEE. The insertion sequence IS1 106 present in the meningococcal genome [60] has also been used as a PCR target for specific and sensitive detection of N meningitidis DNA in CSF specimens [61] and in specimens of peripheral blood [62]. The IS1106-PCR has been adapted to a PCR ELISA format for the diagnosis of meningococcal infection [63,64].…”

Section: Pcr-based Methodsmentioning

confidence: 99%

“…Mammen et al [74] have also reported confirmation of meningococcal infection with a diagnostic nested PCR for porA in urine. An evaluation in England of PCR-based methods which detected ISIIO6 [60,61], class 2/3 OMPs (porB [50]) and siaD genes [49] for diagnosis of meningococcal infections gave a 20% increase in laboratory-ascertained infection [64]. Variable regions within porB encode the class 2 and class 3 proteins and several probes based on these variable regions have been used to identify all the major meningococcal serotypes.…”

Section: Non-culture-based Diagnosis Of N Meningitidis In Clinical Smentioning

confidence: 99%

Molecular typing methods for Neisseria meningitidis

Yakubu¹,

Abadi²,

Pennington³

1999

Journal of Medical Microbiology

View full text Add to dashboard Cite

Neisseria rneningifidis is an important pathogen because it causes life-threatening infections. The rapid course of meningococcal disease and the capacity of some serogroups to cause large-scale epidemics necessitates the use of sensitive, reliable and rapid typing methods to characterise strains. Molecular typing techniques for N. meningifidis are used for epidemiological purposes to investigate outbreaks and the spread of organisms and to examine the population genetic structure of the organism to understand better its variation and evolution. Many investigators have employed molecular typing methods and shown that meningococcal disease is associated with a variety of different epidemiological patterns. The choice of a typing method is dependent upon the epidemiological questions to be answered and on the population genetics of the organism under investigation. With highly clonal populations comprising independent non-recombining lineages such as serogroup A meningococci, ribotyping, multilocus enzyme electrophoresis (MLEE), pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), PCR with arbitrary primers (RAPD) or with other gene-based primers each provides a constant measure of the relationship between strains. A more restricted portfolio of molecular methods -PFGE, MLEE and MLST -is appropriate for the investigation of less clonal serogroup B and C meningococci from localised outbreaks. If a thorough evaluation of the overall population is sought to determine the relationship between new isolates and members of hyper-endemic clonal complexes then quantitative methods such as MLEE and MLST are necessary. Several PCR-based methods are used for the detection and typing of meningococcal strains, many requiring rigorous standardisation before they can be considered suitable for rapid and reliable differentiation between clones. This review examines strain characterisation by molecular techniques and non-culture-based subtyping of meningococci in clinical specimens. It assesses the importance of these techniques and examines the epidemiological questions that they answer and also their limitations.

show abstract

Identification and characterization of a novel insertion sequence, IS1106, downstream of the porA gene in B15 Neisseria meningitidis

Cited by 38 publications

References 43 publications

Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains

Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains

Integration of heterologous DNA into the genome of Paracoccus denitrificans is mediated by a family of IS1248-related elements and a second type of integrative recombination event

Molecular typing methods for Neisseria meningitidis

Contact Info

Product

Resources

About