Identification and characterization of a heat-inducible ftsH gene from tomato (Lycopersicon esculentum Mill.)

Sun, Aijun; Yi, Shuying; Yang, Jinying; Zhao, Chun‐Mei; Liu, Jian

doi:10.1016/j.plantsci.2005.10.010

Cited by 27 publications

(22 citation statements)

References 56 publications

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“…The ‘ Arabidopsis eFP Browser’ (http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi; Winter et al 2007) shows very low expression of FtsH6 in plants grown at standard conditions, and it is increased only after heat treatment. Similar mRNA accumulation was shown for LeFtsH6 , a tomato ortholog of Arabidopsis FtsH6 (Sun et al 2006).…”

Section: Plastidic Ftsh Proteasessupporting

confidence: 76%

FtsH proteases located in the plant chloroplast

Wagner

Aigner

Funk

2012

Physiologia Plantarum

106

127

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FtsHs are a well-characterized family of membrane bound proteases containing an AAA (ATPase associated with various cellular activities) and a Zn(2+) metalloprotease domain. FtsH proteases are found in eubacteria, animals and plants and are known to have a crucial role in housekeeping proteolysis of membrane proteins. In Arabidopsis thaliana, 12 FtsH family members are present (FtsH 1-12) and their subcellular localization is restricted to mitochondria and chloroplasts. In addition, five genes coding for proteins homologous to FtsH (FtsHi 1-5) have been detected in the genome, lacking the conserved zinc-binding motif HEXXH, which presumably renders them inactive for proteolysis. These inactive FtsHs as well as nine of the active FtsHs are thought to be localized in the chloroplast. In this article, we shortly summarize the recent findings on plastidic FtsH proteases in text and figures. We will mainly focus on FtsH 1, 2, 5 and 8 localized in the thylakoid membrane and known for their importance in photosynthesis.

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Section: Plastidic Ftsh Proteasessupporting

confidence: 76%

FtsH proteases located in the plant chloroplast

Wagner

Aigner

Funk

2012

Physiologia Plantarum

106

127

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“…In contrast to other FtsH genes, FtsH6 is rapidly and highly induced by heat stress in Arabidopsis (Fig. 5c) and other species including dicot and monocot crops37383940, indicating evolutionary conservation and ecological relevance of FtsH6 heat inducibility. In accordance with this, our data presented here demonstrate a role of FtsH6 in regulating the stability of HSP21 after a priming heat stress, although the molecular mechanism through which this occurs is not clear at present.…”

Section: Discussionmentioning

confidence: 92%

The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis

2016

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Acquired tolerance to heat stress is an increased resistance to elevated temperature following a prior exposure to heat. The maintenance of acquired thermotolerance in the absence of intervening stress is called ‘thermomemory' but the mechanistic basis for this memory is not well defined. Here we show that Arabidopsis HSP21, a plastidial small heat shock protein that rapidly accumulates after heat stress and remains abundant during the thermomemory phase, is a crucial component of thermomemory. Sustained memory requires that HSP21 levels remain high. Through pharmacological interrogation and transcriptome profiling, we show that the plastid-localized metalloprotease FtsH6 regulates HSP21 abundance. Lack of a functional FtsH6 protein promotes HSP21 accumulation during the later stages of thermomemory and increases thermomemory capacity. Our results thus reveal the presence of a plastidial FtsH6–HSP21 control module for thermomemory in plants.

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“…For example, the soybean hsp17.3B promoter was used to drive gusA expression in the moss Physcomitrella patens resulting in no GUS activity at 25°C but increasing GUS activity at higher temperatures (Saidi et al 2005). Heat shock elements (HSEs) were identified in the tomato ftsH6 promoter, and were recognized by another HSP acting as a transcription factor (heat shock factor, HSF; Sun et al 2006). The heat shock response may be universal or tissue specific.…”

Section: Additional Auxin Response Elements Include the As-1/ocs-limentioning

confidence: 99%

Promoter diversity in multigene transformation

et al. 2010

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Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.

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Identification and characterization of a heat-inducible ftsH gene from tomato (Lycopersicon esculentum Mill.)

Cited by 27 publications

References 56 publications

FtsH proteases located in the plant chloroplast

FtsH proteases located in the plant chloroplast

The plastid metalloprotease FtsH6 and small heat shock protein HSP21 jointly regulate thermomemory in Arabidopsis

Promoter diversity in multigene transformation

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