Identification and characterization of a novel porcine endothelial cell‐specific <scp>T</scp>ie1 promoter

Chen, Tao; Wang, Jiaqiang; Xue, Binghua; Kong, Qingran; Liu, Zhong Hua; Yu, Bo

doi:10.1111/xen.12059

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…TIE1 is an angiopoietin receptor with immunoglobulin and EGF-like domains 1, and the TIE1 protein is a cell surface protein expressed in endothelial cells ( 70 ); however, it is also expressed in immature hematopoietic cells and platelets ( 71 ). TIE1 has an inflammatory effect and may serve a role in mechanotransduction, neovascularization and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma

Chen,

Chuang,

Yang

et al. 2024

Oncol Lett

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma

Chen,

Chuang,

Yang

et al. 2024

Oncol Lett

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“…Porcine iliac endothelial cells is immortalized cell lines purchased from the Type Culture Collection of the Chinese Academy of Sciences, and THP‐1 and PIECs were cultured with RPMI‐1640 containing 10% (vol/vol) FBS, at 37°C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

A potential role of TLR2 in xenograft rejection of porcine iliac endothelial cells: An in vitro study

Chen

Gao

Chen

et al. 2019

Xenotransplantation

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Background Porcine vascular endothelial cells are a major participant in xenograft rejection. The Toll‐like receptor 2 (TLR2) pathway plays an important role in both innate and adaptive immunity. The specific role of TLR2 in the response to a xenograft has not been reported. Whether the TLR2 pathway in pig vascular endothelial cells is involved in acute rejection needs to be investigated, and the mechanism is explored. Methods We used a modified antibody‐dependent complement‐mediated cytotoxicity (ADCC) assay to conduct in vitro experiments. In porcine iliac artery endothelial cells (PIECs), siRNA was used to knock down the expression of TLR2, CXCL8, and CCL2. The effect of human serum or inactivated human serum on the expression of TLR2 was analyzed by real‐time PCR and Western blotting, and transwell assays were used to assess the chemotactic efficiency of PIECs on human monocyte‐macrophages (THP‐1 cells) and human neutrophils. The downstream signaling pathways activated by human serum were detected by Western blotting, and the regulation of proinflammatory chemokines and cytokines by TLR2 signaling was assessed by real‐time PCR and ELISA. Results TLR2 was significantly upregulated in PIECs after exposure to human serum, and porcine proinflammatory chemokines, CXCL8 and CCL2, were induced, at least partially, in a TLR2‐dependent pattern; the upregulated chemokines participated in the chemotaxis of human neutrophils and THP‐1 cells across the species barrier. Conclusions (i) TLR2 is significantly upregulated in PIECs by human serum, (ii) the elevated TLR2 participates in the chemotaxis of inflammatory cells through the secretion of chemokine CCL2 and CXCL8, and (iii) blockade of TLR2 would be beneficial for xenograft survival.

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“…Vascular endothelial cells represent a primary target of genetic modifications in donor pigs for xenotransplantation. In this regard, Chen et al [16] identified the porcine Tie1 (sTie1) promoter as a powerful porcine endothelial cell-specific promoter. Tie1 promoters with different lengths of 5′-regulatory regions were cloned, and major putative DNA-binding motifs were mutated by site-directed mutagenesis.…”

Section: Donor Animalsmentioning

confidence: 99%

Xenotransplantation literature update, November–December 2013

Schneider

2014

Xenotransplantation

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Identification and characterization of a novel porcine endothelial cell‐specific Tie1 promoter

Cited by 4 publications

References 42 publications

Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma

Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma

A potential role of TLR2 in xenograft rejection of porcine iliac endothelial cells: An in vitro study

Xenotransplantation literature update, November–December 2013

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