Identification and characterization of a serotype b-specific segment of the Haemophilus influenzae genome

Ely, Susan; Tippett, J; Moxon, E. Richard

doi:10.1128/iai.57.9.2926-2928.1989

Cited by 10 publications

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“…Both bwas performed in which control and infected tissue were variants (RM2177 and RM132) caused accumulation of deincubated and examined by light microscopy, TEM, and bris and extrusion of epithelial cells. Examination of serial SEM after 30 min, 4 h, 14 h, and 24 h (except for explants 1-,um sections revealed damage that varied from localized infected with Ela/R1369, for which SEM was not conduct-(patchy) to generalized (widespread) loss of epithelial integed).…”

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“…Strain RM2177 has noniridescent colonies, does not react with antiserum to H. influenzae type b, lacks polyribosyl-ribitol phosphate in culture supernatants, and lacks surface immunofluorescence when reacted with fluorescein-conjugated rabbit antiserum to polyribosylribitol phosphate. It is also avirulent in the infant rat model of systemic H. influenzae infection (4). RM132 is a derivative of strain Rd, a btype d strain (32).…”

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“…In contrast, mean damage scores of epithelia infected with the b+ variants were not significantly different from those of controls, though strain RM153 caused a small but significant excess of projecting cells and cytoplasmic blebbing of ciliated cells. When explants were infected with the isogenic pairs and examined at 30 min, 4 h, 14 h, and 24 h, light and electron microscopy revealed that the damage to explants infected with b-but not b+ variants was progressive (maximal by 14 h), with all explants being indistinguishable from controls 30 min and 4 h following the onset of incubation.…”

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Interaction of capsulate Haemophilus influenzae with human airway mucosa in vitro

et al. 1992

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Two pairs of isogenic capsulate and noncapsulate and one pair of capsulate fimbriate and nonfimbriate strains of Haemophilus influenzae type b were studied in an organ culture of human respiratory mucosa. Over 24 h, the numbers of recovered bacteria increased from the original inoculum size of 10i to 108 CFU/ml. Transmission electron microscopy and scanning electron microscopy showed that noncapsulate organisms caused significant epithelial damage, whereas capsulate strains did not. Association of noncapsulate bacteria with damaged epithelial cells was observed by 14 h of incubation. In contrast, capsulate organisms were associated with a dense, thick, gel-like matrix which was observed above the epithelial surface. These capsulate organisms were not seen to associate with the epithelial surface (by transmission electron microscopy), though they were occasionally seen adhering to cells by scanning electron microscopy. Fimbriate capsulate H. influenzae showed increased adherence to buccal cells compared with nonfimbriate capsulate organisms. There was also association of fimbriate capsulate bacteria with damaged organ culture epithelium in one of four experiments. It is concluded that both capsule and fimbriae affect the interaction of H. influenzae with human airway mucosa in vitro by influencing adherence to and damage of the epithelium.

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