Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice

Abstract

We have identified a cell population expressing erythroid (TER-119) and megakaryocyte (4A5) markers in the bone marrow of normal mice. This population is present at high frequency in the marrows and in the spleens involved in the erythroid expansion that occurs in mice recovering from phenylhydrazine (PHZ)-induced hemolytic anemia. TER-119+/4A5+ cells were isolated from the spleen of PHZ-treated animals and were found to be blast-like benzidine-negative cells that generate erythroid and megakaryocytic cells within 24-48 hours of culture in the presence of erythropoietin (EPO) or thrombopoietin (TPO). TER-119+/4A5+ cells represent a late bipotent erythroid and megakaryocytic cell precursors that may exert an important role in the recovery from PHZ-induced anemia.

A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosis

“…However, the molecular and cellular mechanisms through which these cells differentiate and mature remain poorly understood. Mk cells derive from bi-potent erythro-Mk progenitors [ 2 , 3 ]. Committed Mk progenitors undergo endomitosis and become polyploid with multilobated nuclei.…”

Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requiresTtll4

“…, 2007 ). Although platelets and erythrocytes have different functions, both megakaryocytes and erythroid lineages proliferate from common megakaryocyte-erythroid progenitor cells in the bone marrow ( Vannucchi et al. , 2000 ) and show similarities in differentiation, cytology, and homeostatic regulation ( Kaushansky, 2008 ; Klimchenko et al.…”

Protein Kinase C ε in Hematopoiesis: Conductor or Selector?

“…14,18,82 Erythroid and megakaryocytic lineages, driven by the respective specific cytokines EPO and TPO, share similar arrays of transcription factors and several biologic characteristics. 83 Here we extensively discussed PKCε, whose expression is differently modulated by both EPO and TPO, characterizing erythroid and megakaryocytic differentiation. 14,18 The different kinetics of PKCε expression is responsible for independent effects on cell differentiation lineages.…”