Identification and characterization of a new base substitution in the vaccine strain of sabin 3 poliovirus

Weeks‐Levy, Carolyn; Tatem, Joanne M.; Dimichele, Susan J.; Waterfield, W. F.; Georgiu, Alice; Mento, Steven J.

doi:10.1016/0042-6822(91)90576-w

Cited by 34 publications

(29 citation statements)

References 5 publications

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“…The high proportion of S2 isolates with a nonsynonymous mutation at the corresponding codon indicates great genetic instability of this serotype, in accordance with data from several previous reports (5,22,23). Most S3 isolates were mutated at T 6 (T3I), but the value of this attenuation marker is controversial (3,32,33), as such substitutions have been found in three substrains of OPV that were used without any reported adverse reactions (28).…”

Section: S3/s2supporting

confidence: 85%

“…Among the mutations observed in the VP1 regions, special attention was paid to nonsynonymous mutations affecting codons of amino acid residues that are known to be implicated in the attenuation phenotype of OPV strains (attenuation markers): VP1 residues A 88 , T 106 , and F 134 of the serotype 1 Sabin strain (S1) (2); VP1 residue I 143 of S2 (22); and VP1 residue T 6 of S3 (32,33) (Fig. 2).…”

Section: Detection Of Pvmentioning

confidence: 99%

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Environmental Poliovirus Surveillance during Oral Poliovirus Vaccine and Inactivated Poliovirus Vaccine Use in Córdoba Province, Argentina

Mueller

Bessaud

Huang

et al. 2009

Appl Environ Microbiol

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During an intermittent switch from IPV to OPV, this proportion increased to 100% within 2 months. During the 3 months when IPV was reintroduced to replace OPV, a substantial proportion of samples (25%) remained positive for poliovirus. In the OPV-using sites, on average, 54% of samples were poliovirus positive. Seventy-seven percent of poliovirus isolates showed at least one mutation in the VP1-encoding sequence; the maximum genetic divergence from the Sabin strain was 0.7%. Several isolates showed mutations on attenuation markers in the VP1-encoding sequence. The frequency or type of virus mutation did not differ between periods of IPV and OPV use or by virus serotypes. This study indicates that the sustained transmission of OPV viruses was limited during IPV use in a middle-income country with a temperate climate. The continued importation of poliovirus and genetic instability of vaccine strains even in the absence of sustained circulation suggest that high poliovirus vaccine coverage has to be maintained for all countries until the risk of reintroduction of either wild or vaccine-derived poliovirus is close to zero worldwide.

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Section: S3/s2supporting

confidence: 85%

Section: Detection Of Pvmentioning

confidence: 99%

Environmental Poliovirus Surveillance during Oral Poliovirus Vaccine and Inactivated Poliovirus Vaccine Use in Córdoba Province, Argentina

Mueller

Bessaud

Huang

et al. 2009

Appl Environ Microbiol

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“…143 in S2 (32), and T 6 in S3 (33,34). In the VP1 sequence of the 23 S1 isolates, we found 12 isolates with an amino acid substitution at T 106 (52%) (Fig.…”

Section: Isolation Of Pvs From Sewage Watermentioning

confidence: 99%

Environmental Surveillance of Poliovirus in Sewage Water around the Introduction Period for Inactivated Polio Vaccine in Japan

Nakamura

Hamasaki

Yoshitomi

et al. 2015

Appl Environ Microbiol

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Environmental virus surveillance was conducted at two independent sewage plants from urban and rural areas in the northern prefecture of the Kyushu district, Japan, to trace polioviruses (PVs) within communities. Consequently, 83 PVs were isolated over a 34-month period from April 2010 to January 2013. The frequency of PV isolation at the urban plant was 1.5 times higher than that at the rural plant. Molecular sequence analysis of the viral VP1 gene identified all three serotypes among the PV isolates, with the most prevalent serotype being type 2 (46%). Nearly all poliovirus isolates exhibited more than one nucleotide mutation from the Sabin vaccine strains. During this study, inactivated poliovirus vaccine (IPV) was introduced for routine immunization on 1 September 2012, replacing the live oral poliovirus vaccine (OPV). Interestingly, the frequency of PV isolation from sewage waters declined before OPV cessation at both sites. Our study highlights the importance of environmental surveillance for the detection of the excretion of PVs from an OPV-immunized population in a highly sensitive manner, during the OPV-to-IPV transition period.

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“…Thus the largest part of the attenuated phenotype of the type 3 Sabin vaccine strain of poliovirus was attributable to only two mutations. Subsequently a third mutation at nucleotide 2493, which is believed to have an attenuating effect, was identified by other workers, leading to an amino acid change from isoleucine to threonine at residue 6 of capsid protein VP 1 (Weeks- Levy et al, 1991 ;Tatem et al, 1992). This difference was not observed in our original clones probably because the virus from which they were derived has been plaque-purified and passaged twice in HEp2C cells, and it has been shown that the mutation is rapidly lost on passage.…”

Section: The Molecular Basis Of the Attenuation And Reversion Of The mentioning

confidence: 99%

The molecular biology of poliovaccines

Minor

1992

Journal of General Virology

200

134

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Identification and characterization of a new base substitution in the vaccine strain of sabin 3 poliovirus

Cited by 34 publications

References 5 publications

Environmental Poliovirus Surveillance during Oral Poliovirus Vaccine and Inactivated Poliovirus Vaccine Use in Córdoba Province, Argentina

Environmental Poliovirus Surveillance during Oral Poliovirus Vaccine and Inactivated Poliovirus Vaccine Use in Córdoba Province, Argentina

Environmental Surveillance of Poliovirus in Sewage Water around the Introduction Period for Inactivated Polio Vaccine in Japan

The molecular biology of poliovaccines

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