Identification and characterization of a major subgroup of conjugative Campylobacter jejuni plasmids

Schmidt-Ott, Ruprecht; Pohl, Sven; Burghard, Sebastian; Weig, Michael; Groß, Uwe

doi:10.1016/j.jinf.2004.02.013

Cited by 33 publications

(32 citation statements)

References 34 publications

(24 reference statements)

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“…Since this paper was first submitted a study was published showing that 16 out of 56 clinical isolates of C. jejuni from the area of Göttingen in Germany harbour plasmids varying in size from 6 to 66 kb (Schmidt-Ott et al, 2004). Only one of these plasmids was a homologue of pVir, the virulence plasmid previously characterized by Bacon et al (2002).…”

Section: Discussionmentioning

confidence: 99%

Nucleotide sequences and comparison of two large conjugative plasmids from different Campylobacter species

Batchelor

2004

Microbiology

130

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Section: Discussionmentioning

confidence: 99%

Nucleotide sequences and comparison of two large conjugative plasmids from different Campylobacter species

Batchelor

2004

Microbiology

130

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“…Conjugation experiments were carried out as described by Schmidt-Ott et al (2005) with the modification that erythromycin was used as the antimicrobial selected by recipient strains instead of nalidixic acid. As recipient strains, two erythromycin-resistant C. jejuni strains of clinical origin were selected and named as Rec1 and Rec2 (Table 2).…”

Section: Methodsmentioning

confidence: 99%

Molecular mechanisms of quinolone, macrolide, and tetracycline resistance amongCampylobacterisolates from initial stages of broiler production

et al. 2014

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The aim of this study was to investigate the resistance mechanisms of quinolones, macrolides and tetracycline in campylobacter isolates from grandparent and parent broiler breeders in Spain. Twenty-six isolates were investigated for quinolone resistance, three isolates for macrolide resistance and 39 for tetracycline resistance. All of the quinolone-resistant isolates possessed the mutation Thr86Ile in the quinolone resistance-determining region of gyrA and one isolate possessed the mutation Pro104Ser. Only one Campylobacter coli population (defined by restriction fragment length polymorphism-polymerase chain reaction of flaA and pulsed field gel electrophoresis) was resistant to erythromycin, and the mutation A2075G (23S rDNA) was responsible for macrolide resistance. The tetO gene was found in all of the tetracycline-resistant isolates. Twenty-two out of the 39 isolates investigated by Southern blot possessed chromosomic location of tetO and 17 were located on plasmids. Most of the plasmids with tetO were of around 60 kb and conjugation was demonstrated in a selection of them. In conclusion, we showed that Thr86Ile is highly prevalent in quinolone-resistant isolates as well as mutation A2075G in macrolide-resistant isolates of poultry origin. More variability was found for tetO. The possibility of horizontal transmission of tetO among campylobacter isolates is also an issue of concern in public health.

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“…The C. jejuni strains used in this study were B2, which was isolated in the University Medical Center Göttingen from a patient suffering from gastroenteritis (Schmidt-Ott et al, 2005;Dasti et al, 2007), and the C. jejuni strains 81-176 and NCTC 11168. Bacteria were routinely grown on Columbia blood agar supplemented with 5 % defibrinated sheep blood under microaerophilic conditions (85 % N 2 , 10 % CO 2 , 5 % O 2 ) at 42 uC for 24 h. When needed, appropriate antibiotics were supplemented at the following concentrations: kanamycin (50 mg ml 21 ) or chloramphenicol (30 mg ml 21 ).…”

Section: Methodsmentioning

confidence: 99%

Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells

et al. 2010

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Campylobacter jejuni, an important food-borne bacterial pathogen in industrialized countries and in the developing world, is one of the major causes of bacterial diarrhoea. To identify genes which are important for the invasion of host cells by the pathogen, we screened altogether 660 clones of a transposon-generated mutant library based on the clinical C. jejuni isolate B2. Thereby, we identified a clone with a transposon insertion in gene cj0952c. As in the well-characterized C. jejuni strain NCTC 11168, the corresponding protein together with the gene product of the adjacent gene cj0951c consists of two transmembrane domains, a HAMP domain and a putative MCP domain, which together are thought to act as a chemoreceptor, designated Tlp7. In this report we show that genes cj0952c and cj0951c (i) are important for the host cell invasion of the pathogen, (ii) are not translated as one protein in C. jejuni isolate B2, contradicting the idea of a postulated read-through mechanism, (iii) affect the motility of C. jejuni, (iv) alter the chemotactic behaviour of the pathogen towards formic acid, and (v) are not related to the utilization of formic acid by formate dehydrogenase.

show abstract

Identification and characterization of a major subgroup of conjugative Campylobacter jejuni plasmids

Cited by 33 publications

References 34 publications

Nucleotide sequences and comparison of two large conjugative plasmids from different Campylobacter species

Nucleotide sequences and comparison of two large conjugative plasmids from different Campylobacter species

Molecular mechanisms of quinolone, macrolide, and tetracycline resistance amongCampylobacterisolates from initial stages of broiler production

Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells

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