Identification and characterization of a new conjugation/type IVA secretion system (trb/tra) of Legionella pneumophila Corby localized on two mobile genomic islands

Glöckner, Gernot; Albert-Weißenberger, Christiane; Weinmann, Erik; Jacobi, Sebastian; Schunder, Eva; Steinert, Michael; Hacker, Jörg; Heuner, Klaus

doi:10.1016/j.ijmm.2007.07.012

Cited by 113 publications

(121 citation statements)

References 61 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…6C). This result again indicates that Trb-1 is able to be transferred horizontally between Legionella strains, as it was shown recently by an in vitro experiment (Glöckner et al, 2008). Using inverse PCR, we demonstrated that region 2/IV is part of the Lvh island and that both islands (Trb-1 and Lvh) can also exist in their excised episomal form (data not shown), as it is described for L. pneumophila (Glöckner et al, 2008;DoleansJordheim et al, 2006).…”

Section: General Features Of the Genomesupporting

confidence: 88%

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

Brzuszkiewicz

Schulz

Rydzewski

et al. 2013

International Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Legionella oakridgensis is able to cause Legionnaires` disease, but is less virulent compared to L. pneumophila strains and very rarely associated with human disease. L. oakridgensis is the only species of the family legionellae which is able to grow on media without additional cysteine. In contrast to earlier publications, we found that L. oakridgensis is able to multiply in amoebae. We sequenced the genome of L. oakridgensis type strain OR-10 (ATCC 33761).The genome is smaller than the other yet sequenced Legionella genomes and has a higher G+C-content of 40.9%. L. oakridgensis lacks a flagellum and it also lacks all genes of the flagellar regulon except of the alternative sigma-28 factor FliA and the anti-sigma-28 factor

show abstract

Section: General Features Of the Genomesupporting

confidence: 88%

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

Brzuszkiewicz

Schulz

Rydzewski

et al. 2013

International Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cloning and Reverse Transcription-PCR-The BLASTP algorithm (37) was used to identify orthologues of V. cholerae O1 biovar El Tor CqsA (NP_232914) and CqsS (NP_232913), encoded in the genomes of the L. pneumophila strains Philadelphia-1 (38), Paris, Lens (39), and Corby (40). DNA manipulations were performed according to standard protocols, and plasmids were isolated using commercially available kits (Qiagen, Macherey-Nagel).…”

Section: Methodsmentioning

confidence: 99%

The Legionella Autoinducer Synthase LqsA Produces an α-Hydroxyketone Signaling Molecule

Spirig

Tiaden

Kiefer

et al. 2008

Journal of Biological Chemistry

107

114

View full text Add to dashboard Cite

The opportunistic pathogen Legionella pneumophila replicates in human lung macrophages and in free-living amoebae. To accommodate the transfer between host cells, L. pneumophila switches from a replicative to a transmissive phase. L. pneumophila harbors a gene cluster homologous to the Vibrio cholerae cqsAS quorum sensing system, encoding a putative autoinducer synthase (lqsA) and a sensor kinase (lqsS), which flank a response regulator (lqsR). LqsR is an element of the L. pneumophila virulence regulatory network, which promotes pathogen-host cell interactions and inhibits entry into the replicative growth phase. Here, we show that lqsA functionally complements a V. cholerae cqsA autoinducer synthase deletion mutant and, upon expression in L. pneumophila or Escherichia coli, produces the diffusible signaling molecule LAI-1 (Legionella autoinducer-1). LAI-1 is distinct from CAI-1 (Cholerae autoinducer-1) and was identified as 3-hydroxypentadecan-4-one using liquid chromatography coupled to high resolution tandem mass spectrometry. The activity of both LqsA and CqsA was abolished upon mutation of a conserved lysine, and covalent binding of the cofactor pyridoxal 5-phosphate to this lysine was confirmed by mass spectrometry. Thus, LqsA and CqsA belong to a family of pyridoxal 5-phosphate-dependent autoinducer synthases, which produce the ␣-hydroxyketone signaling molecules LAI-1 and CAI-1.

show abstract

“…It seems as if fliA belongs to the class III genes in strain Paris, but is not directly linked to the flagellar regulon in strain Corby (Albert-Weissenberger et al 2010;Jacobi et al 2004). Both strains are virulent L. pneumophila Sg1 isolates with only small differences within their genomes (Cazalet et al 2004;Jepras et al 1985;Glöckner et al 2008). Our intention was to clarify the regulation of fliA expression in L. pneumophila and to examine the differences between both strains.…”

Section: Introductionmentioning

confidence: 99%

FliA expression analysis and influence of the regulatory proteins RpoN, FleQ and FliA on virulence and in vivo fitness in Legionella pneumophila

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

Identification and characterization of a new conjugation/type IVA secretion system (trb/tra) of Legionella pneumophila Corby localized on two mobile genomic islands

Cited by 113 publications

References 61 publications

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

Legionella oakridgensis ATCC 33761 genome sequence and phenotypic characterization reveals its replication capacity in amoebae

The Legionella Autoinducer Synthase LqsA Produces an α-Hydroxyketone Signaling Molecule

FliA expression analysis and influence of the regulatory proteins RpoN, FleQ and FliA on virulence and in vivo fitness in Legionella pneumophila

Contact Info

Product

Resources

About