Identification and Characterization of a Novel Line of Drosophila Schneider S2 Cells That Respond to Wingless Signaling

Yanagawa, Shin-ichi; Lee, Jong-Seo; Ishimoto, Akinori

doi:10.1074/jbc.273.48.32353

Cited by 166 publications

(174 citation statements)

References 35 publications

(43 reference statements)

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“…Cell Cultures-S 2 R ϩ cells (33) were cultured in Schneider's insect medium supplemented with 10% heat-inactivated FBS and kept in 75-cm 2 T flasks or in tissue culture dishes (245 ϫ 245 ϫ 25 mm) at 25°C.…”

Section: Methodsmentioning

confidence: 99%

Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster

Stockum

Basso

Petronilli

et al. 2011

Journal of Biological Chemistry

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Background:We have studied the properties of Ca 2ϩ transport in Drosophila mitochondria. Results: Drosophila mitochondria possess Ca 2ϩ transport systems matching their mammalian equivalents but have a unique selective Ca 2ϩ release channel that does not mediate swelling. Conclusion:The Drosophila Ca 2ϩ release channel is involved in Ca 2ϩ homeostasis rather than cell death. Significance: This channel may represent the missing link between the permeability transition pore of yeast and mammals.

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Section: Methodsmentioning

confidence: 99%

Properties of Ca2+ Transport in Mitochondria of Drosophila melanogaster

Stockum

Basso

Petronilli

et al. 2011

Journal of Biological Chemistry

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“…PC12 cell lines expressing wild-type and mutant VCPs under the tet-off promoter were established following a standard procedure as described previously (40). S2Rϩ cells (41) were maintained in Schneider's Drosophila medium with 10% fetal bovine serum. When needed, cells were transfected with expression plasmids using Lipofectamine Plus, Lipofectamine 2000TM, or Cellfectin (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

Valosin-containing Protein (VCP) in Novel Feedback Machinery between Abnormal Protein Accumulation and Transcriptional Suppression

Koike

Fukushi

Ichinohe

et al. 2010

Journal of Biological Chemistry

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Abnormal protein accumulation is often observed in human neurodegenerative disorders such as polyglutamine diseases and Parkinson disease. Genetic and biochemical analyses indicate that valosin-containing protein (VCP) is a crucial molecule in the pathogenesis of human neurodegenerative disorders. We report here that VCP was specifically modified in neuronal cells with abnormal protein accumulation; this modification caused the translocation of VCP into the nucleus. Modification-mimic forms of VCP induced transcriptional suppression with deacetylation of core histones, leading to cell atrophy and the decrease of de novo protein synthesis. Preventing VCP nuclear translocation in polyglutamine-expressing neuronal cells and Drosophila eyes mitigated neurite retraction and eye degenerations, respectively, concomitant with the recovery of core histone acetylation. This represents a novel feedback mechanism that regulates abnormal protein levels in the cytoplasm during physiological processes, as well as in pathological conditions such as abnormal protein accumulation in neurodegenerations.

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“…In S2Rϩ cells (Yanagawa et al, 1998), Su(H)-dependent Notch signalling was measured with NRE or a NME (Perez et al, 2005). For each transfection, the ratio of Luciferase reporter (NRE or NME), normalization vector (pPOLIII-Renilla), Inducer (pPacNotch, pPac-NICD, pPac-TNotch, or pPAC-CeN) DNA was 1:1:4, with a total of 150 ng DNA added per well.…”

Section: Gain Of Function Assay For Su(h)-dependent Notch Signallingmentioning

confidence: 99%

“…The molecular nature of this regulatory event was characterized further by testing the ability of various forms of Notch to modulate Wingless signalling in S2Rϩ cells, a Drosophila cell line that expresses the DFrizzled 2 (DFz2) receptor and responds to Wingless (Yanagawa et al, 1998;Fig. 1C).…”

Section: Various Forms Of Notch Can Regulate Wnt Signalling By Targetmentioning

confidence: 99%