Identification and characterization of a phytoplasma disease of jute (Corchorus olitorius L.) from south-western Turkey

Özdemir, Zahide; Çağırgan, M. İlhan

doi:10.1016/j.cropro.2015.03.018

Cited by 13 publications

(9 citation statements)

References 28 publications

(22 reference statements)

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“…orientalis collected from Antalya was 99.3% and 99.6%, respectively. The sesame phyllody strains GPS16 from sesame and JPHY3 from jute ( Corchorus olitorius ) (Özdemir & Cagirgan, ) plants showed 99.5% similarity with strain Nh50. Subgroup II‐B and II‐C members shared 97.7%–97.8% sequence homology with strain Nh50.…”

Section: Resultsmentioning

confidence: 99%

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Identification of phytoplasmas from Neoaliturus haematoceps associated with sesame phyllody disease in southwestern Turkey

Özdemir

2018

Journal of Phytopathology

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Phytoplasmas were detected based on nested PCR of the F2nR2 region of the 16S rDNA from Neoaliturus haematoceps (Mulsant and Rey) (Family: Cicadellidae). A total of 65 insect samples collected from sesame fields in Antalya, Turkey, during 2012–2014 were tested for phytoplasma detection. Phytoplasmas detected in fifteen samples showed an amplicon approximately 1250 bp in size using the universal primers of P1/P7 and R16F2n/R16R2. Identification of the phytoplasmas by sequence analysis revealed three different 16S rDNA phytoplasma groups: the peanut witches’‐broom, group II; clover proliferation, group VI; and pigeon pea witches’‐broom, group IX. The molecular characterization of subgroups was determined by sequence analysis and PCR‐RFLP using the restriction enzymes RsaI and TaqI. Restriction profiles of the subgroups were also confirmed using the iPhyclassifier program. BLAST and PCR‐RFLP analyses classified the subgroups as II‐D, VI‐A and IX‐C. This is the first report of molecular detection of three 16S rDNA subgroups of phytoplasmas, II‐D, VI‐A and IX‐C, from N. haematoceps in Turkey. This study also supports earlier studies of sesame phyllody phytoplasmas by N. haematoceps.

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Section: Resultsmentioning

confidence: 99%

“…DNA was extracted individually from each adult insect specimen according to Özdemir and Cagirgan () with modifications. DNA presence was confirmed by 1% agarose gel electrophoresis, and the amount of DNA was also measured spectrophotometrically.…”

Section: Methodsmentioning

confidence: 99%

Identification of phytoplasmas from Neoaliturus haematoceps associated with sesame phyllody disease in southwestern Turkey

Özdemir

2018

Journal of Phytopathology

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“…The new data is important for the locality in terms of agent/disease transmission. In Turkey, Ayvaci et al, (2020), Özdemir and Cagirgan (2015), and Ozdemir et al, (2014) described this pathogen in alfalfa (Medicago sativa), jute (Corchorus olitorius) plants exhibiting symptoms such as little leaves, leaf yellowing, and witches' broom and in Orosius orientalis, respectively. The molecular characterization of this strain in tomato affirmed the 'Ca.…”

Section: Discussionmentioning

confidence: 99%

First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye

Usta

Güller

Sipahioğlu

2023

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye.

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“…Also, Biswas et al (2014) discovered that C. olitorius plants have phytoplasmas associated with symptoms like small leaves and bunchy tops. Jute plants were identified by Ozdemir & Cagirgan (2015) as weeds in experimental sesame trials carried out at the Akdeniz University campus in Antalya. Weed characteristics included abnormally formed flowers, smaller leaves with short internodes, and yellowing.…”

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Characterization of Phytoplasma and its Effect on the Vitamin and Fibers in Corchorus olitorius Crops in Egypt

Abdel Rehium,

EL-Abagy,

Mokbel

et al. 2024

Egypt. J. Agron.

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A PHYTOPLASMA was detected in the Corchorus olitorius (Mallow) crop in 2022 at Fayoum governorate, Egypt. Infected C. olitorius showed symptoms of phyllody, leaf deformations, and slight yellowing. Total DNA was extracted from symptomatic or asymptomatic leaf tissues and was tested for phytoplasma presence using nested polymerase chain reaction (PCR). A fragment was amplified from symptomatic samples using universal phytoplasma primer set P1/P7 and primer set R16F2n/R16R2. The amplicon for these primers is 1271 bp, but the DNA of asymptomatic samples did not yield an amplicon. The nested PCR products were sequenced with R16F2n/ R16R2 primers, and then sequences were aligned through the DNA-man program V7. Based on the phytoplasma found in this study (Acc. No. OQ836595), the results imply 98.1% sequence identity with the phytoplasma of C. olitorius (OP889144) from Egypt, China (OP889144 and KM103730), and from other Indian crops. Identifying the vector species that is spreading the discovered phytoplasma in the Mallow crop is still necessary. Many active phytochemicals found in C. olitorius plants are of great interest due to their potential to improve human health. This study demonstrates that in plant leaves, phytoplasma presence lowered the tocopherol (Vitamin E) content and increased the crude fiber content.

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Identification and characterization of a phytoplasma disease of jute (Corchorus olitorius L.) from south-western Turkey

Cited by 13 publications

References 28 publications

Identification of phytoplasmas from Neoaliturus haematoceps associated with sesame phyllody disease in southwestern Turkey

Identification of phytoplasmas from Neoaliturus haematoceps associated with sesame phyllody disease in southwestern Turkey

First Report of ‘Candidatus Phytoplasma australasia’ Strain Related to Witches’-Broom of Tomato in Türkiye

Detection and Molecular Characterization of Phytoplasma and its Effect on the Vitamin and Fibers in Corchorus olitorius Crops in Egypt

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