Identification and characterization of a novel KG42 xylanase (GH10 family) isolated from the black goat rumen-derived metagenomic library

Xylanolytic Extremozymes Retrieved From Environmental Metagenomes: Characteristics, Genetic Engineering, and Applications

“…The success rate of functional screening of libraries is significantly higher than the sequence-based approaches in obtaining more positive clones as the latter provides usual partial sequences (Lorenz and Eck, 2005;Berini et al, 2017). Several such metagenomic libraries have been constructed in plasmids (Verma et al, 2013b), cosmids (Mo et al, 2010;Chang et al, 2011;Bao et al, 2012), and fosmids (Jeong et al, 2012;Knapik et al, 2019) for retrieving genes that encode xylan-degrading enzymes (Wang et al, 2015;Kim et al, 2018;Thornbury et al, 2019). Among them, a majority of the metagenomic libraries have been constructed in the fosmid vectors (pCC2FOS TM and pCC1FOS TM ) with its host strain EPI300-T1R TM , provided by Epicentre, for obtaining genes encoding xylan-degrading enzymes (Jeong et al, 2012;Kim et al, 2018;Liu et al, 2019).…”

“…Fosmids can harbor large stretches of DNA (40-60 kb) that enhance the number of positive hits. Alves et al (2020) report (Kim et al, 2018). In another report, a pCT3FK fosmid vector was used to construct 1,50,000 clones, and one positive clone (XylA3) has been identified for xylanase activity (Knapik et al, 2019 (Lee et al, 2006;Wang et al, 2015).…”

“…Further, Zhang et al (2014) studied the role of human gut inhabitant Bacteroidetes by reporting two GH-10 family endoxylanases from the human gut metagenome. Several new findings associated with the xylan-depolymerizing enzymes of metagenomic origin have been published during the past 5 years ( Wang et al, 2015 ; Rashamuse et al, 2016 ; Kim et al, 2018 ; Ellila et al, 2019 ; Alves et al, 2020 ; Victorica et al, 2020 ; Tables 1 , 2 ). Recently, a novel computational method, thermal activity prediction for xylanase (TAXyl), was developed to predict the thermal activity of GH-10 and GH-11 xylanases ( Shahraki et al, 2019 ).…”

“…Similarly, 17 novel positive xylanolytic clones were obtained out of 1,15,200 clones from a fosmid-based library (average inert size 30.5 kb). Of the 17 clones, one clone, KG42, revealed the highest xylanase (GH-10 family) activity under acidic conditions ( Kim et al, 2018 ). In another report, a pCT3FK fosmid vector was used to construct 1,50,000 clones, and one positive clone (XylA3) has been identified for xylanase activity ( Knapik et al, 2019 ).…”

Characteristics of a XIP‐resistant xylanase from Neocallimastix sp. GMLF1 and its advantage in barley malt saccharification

“…Both Xyn1B and NpXyn11A were from the genus Neocallimastix , which commonly existed in rumen. These microbes secrete a variety of enzymes including xylanases to break down plant cell walls for food nutrients (Kim et al ., ). Microbes with a xylanase resistant against xylanase inhibitor proteins in plants might have more chance to thrive in rumen microbiota, so the rumen microbiota might be a source for us to find new inhibitor protein‐resistant xylanases.…”