Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii

Thongbuakaew, Tipsuda; Suwansa‐ard, Saowaros; Sretarugsa, Prapee; Sobhon, Prasert; Cummins, Scott F.

doi:10.1016/j.aquaculture.2019.04.002

Cited by 24 publications

(32 citation statements)

References 62 publications

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“…It has been reported that 5-HT stimulates the release of neurohormones, including the crustacean hyperglycemic hormone, red pigment-dispersing hormone, neurodepressing hormone, molt-inhibiting hormone, and red pigment-concentrating hormone in the red swamp crayfish, Procambarus clarkii (57-59) and the white Pacific shrimp, Litopenaeus vannamei (60). Recent studies also demonstrate that 5-HT can promote ovarian MroCFSHs expression in the giant freshwater prawn, Macrobrachium rosenbergii (61). Therefore, the fluctuation Sp-CFSH expression during ovarian development may be influenced by other endocrine regulators, such as 5-HT.…”

Section: Discussionmentioning

confidence: 97%

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

et al. 2020

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Crustacean female sex hormone (CFSH) is a key regulator of crustacean sex differentiation. The expression of Sp-CFSH in the mud crab Scylla paramamosain showed a tissue-specific and gender-variant pattern. To explore the role of DNA methylation in Sp-CFSH expression, the 5 ′-flanking region of Sp-CFSH was cloned, and one CpG island containing 12 CpG sites was found. Results of sodium bisulfite sequencing and methylated DNA immunoprecipitation showed that CpG island methylation was stable in the eyestalk ganglion during ovarian development of the females, which was significantly lower than that in the muscle of the females and in the eyestalk ganglion of the males. Such results suggested that the involvement of DNA methylation in regulating Sp-CFSH expression followed an eyestalk ganglion-specific and gender-variant pattern. The analysis of CpG dinucleotide site methylation and activity of the site-directed mutation (SDM) reporter vector further demonstrated that methylation inhibited Sp-CFSH expression by blocking the binding of transcription factor Sp1. The finding suggested for the first time the involvement of CpG methylation in the regulation of Sp-CFSH expression.

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Section: Discussionmentioning

confidence: 97%

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

et al. 2020

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“…The protocol for ovarian tissue culture used in this study was based on Merlin et al 36 and Thongbuakaew et al 37 . Briefly, fragments of stage 3 ovaries, a late developing stage (0.2–0.3 g) based on the classification by Rasolofonirina et al 34 obtained from female H. scabra (n = 3) were dissected and washed in Leibovitz's L-15 medium (Gibco, Grand Island, NY, USA) containing 1000 IU/ml penicillin and 1000 μg/ml streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation

Thongbuakaew

Suwansa‐ard

Chaiyamoon

et al. 2021

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The sea cucumber Holothuria scabra is an economically valuable marine species which is distributed throughout the Asia–Pacific region. With the natural population declining due to over fishing, aquaculture of this species is deemed necessary. Hence, it is essential to understand the mechanisms regulating the reproduction in order to increase their populations. Sex steroids, including estrogens, androgens and progestogens, play an important role in reproduction in most vertebrates and several invertebrates. It has been proposed that sea cucumbers have the same sex steroids as vertebrates but the steroidogenic pathway in the sea cucumbers is still unclear. In this study, we demonstrated by using liquid chromatography-tandem mass spectrometry (LC–MS/MS) that sex steroids (estradiol, progesterone, and testosterone) were present in H. scabra neural and gonadal tissues. In silico searches of available sea cucumber transcriptome data identified 26 steroidogenesis-related genes. Comparative analysis of encoded proteins for the steroidogenic acute regulatory protein (HscStAR), CYP P450 10, 17 and 3A (HscCYP10, HscCYP17, HscCYP3A) and hydroxysteroid dehydrogenases (Hsc3β-HSD, Hsc17β-HSD) with other species was performed to confirm their evolutionary conservation. Gene expression analyses revealed widespread tissue expression. Real-time PCR analysis revealed that HscStAR, HscCYP10, Hsc3β-HSD, and Hsc17β-HSD gene expressions were similar to those in ovaries and testes, which increased during the gonad maturation. HscCYP17 mRNA was increased during ovarian development and its expression declined at late stages in females but continued high level in males. The expression of the HscCYP3A was high at the early stages of ovarian development, but not at other later stages in ovaries, however it remained low in testes. Moreover, a role for steroids in reproduction was confirmed following the effect of sex steroids on vitellogenin (Vtg) expression in ovary explant culture, showing upregulation of Vtg level. Collectively, this study has confirmed the existence of steroids in an echinoderm, as well as characterizing key genes associated with the steroidogenic pathway. We propose that sex steroids might also be associated with the reproduction of H. scabra, and the identification of biosynthetic genes enables future functional studies to be performed.

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“…CFSH has been identified in several decapod species including C. sapidus (Zmora and Chung, 2014), S. verreauxi (Ventura et al, 2014), C. quadricarinatus (Nguyen et al, 2016), P. clarkii (Veenstra, 2015), Chorismus antarcticus (Toullec et al, 2017), M. japonicus (Kotaka and Ohira, 2018), S. paramamosain , and M. rosenbergii (Thongbuakaew et al, 2019). It has been reported that CFSH plays an important role in the development of reproductive phenotypes of female blue crab (C. sapidus) (Zmora and Chung, 2014).…”

Section: Cfshmentioning

confidence: 99%

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

et al. 2021

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Crustacean culture has been developing rapidly in various parts of the world. Therefore, it is important to understand their reproductive biology. Insulin-like androgenic gland hormone (IAG) secreted from the androgenic gland (AG) is widely accepted as a key regulator of sexual differentiation in male crustaceans. However, recently several sex-related genes (i.e., CFSH, DEAD-box family, Tra-2, Sxl, Dsx, Fem-1, Sox gene family, Foxl2, and Dmrt gene family) have been identified via transcriptomic analysis in crustaceans, indicating that sexual differentiation in crustaceans is more complicated than previously expected. It has been found that several non-coding RNAs (i.e., miRNAs, lncRNAs, and piRNAs) and IAG receptors may be involved in the sexual development of decapods. Identification and study of the regulation mechanism of sex-related genes, non-coding RNAs, and IAG receptors will provide valuable information regarding sexual development in decapods. In this review, the roles of hormonal and genetic factors in both males and females are discussed. In males, crustacean female sex hormone (CFSH), Sxl, Dmrt gene family, Dsx, Sox gene family, GEM, Fem-1, l-GnRH-III, and corazonin play important roles in IAG regulation in the “eyestalk-IAG-testis” endocrine axis. Unlike males, the regulation mechanism and interaction of sexual genes are relatively unknown in females. However, CFSH, IAG, Fem-1, FAMeT, Slo, UCHLs, Erk2, Cdc2, EGFR, Vg, VgR, and VIH seem to play crucial roles during ovarian development. This study summarizes the available information in the field, highlights gaps, and lays the foundations for further studies and a better understanding of the regulatory mechanism of sexual development in decapods.

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Identification and characterization of a crustacean female sex hormone in the giant freshwater prawn, Macrobrachium rosenbergii

Cited by 24 publications

References 62 publications

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

DNA Methylation Inhibits the Expression of CFSH in Mud Crab

Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation

The Regulatory Mechanism of Sexual Development in Decapod Crustaceans

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