Identification and Characterization of 2′-Deoxyuridine from the Supernatant of Conidial Suspensions of Rice Blast Fungus as an Infection-Promoting Factor in Rice Plants

Ando, Sugihiro; Sato, Yuko; Shigemori, Hideyuki; Shimizu, Takafumi; Okada, Kazunori; Yamane, Hisakazu; Kamiya, Yûji; Yamada, Kosumi; Akimoto-Tomiyama, Chiharu; Tanabe, Shigeru; Minami, Eiichi

doi:10.1094/mpmi-07-10-0172

Cited by 5 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…‘Nipponbare’) were used in this study. Rice plants were grown in a chamber following previously described protocols [38]. The blast fungus M. oryzae strain Ina86–137 (MAFF 101511, race, 007.0, virulent to Nipponbare) was used for infection, and water was adopted as a mock treatment in this study.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice

et al. 2014

Self Cite

View full text Add to dashboard Cite

WRKY transcription factors and mitogen-activated protein kinase (MAPK) cascades have been shown to play pivotal roles in the regulation of plant defense responses. We previously reported that OsWRKY53-overexpressing rice plants showed enhanced resistance to the rice blast fungus. In this study, we identified OsWRKY53 as a substrate of OsMPK3/OsMPK6, components of a fungal PAMP-responsive MAPK cascade in rice, and analyzed the effect of OsWRKY53 phosphorylation on the regulation of basal defense responses to a virulence race of rice blast fungus Magnaporthe oryzae strain Ina86-137. An in vitro phosphorylation assay revealed that the OsMPK3/OsMPK6 activated by OsMKK4 phosphorylated OsWRKY53 recombinant protein at its multiple clustered serine-proline residues (SP cluster). When OsWRKY53 was coexpressed with a constitutively active mutant of OsMKK4 in a transient reporter gene assay, the enhanced transactivation activity of OsWRKY53 was found to be dependent on phosphorylation of the SP cluster. Transgenic rice plants overexpressing a phospho-mimic mutant of OsWRKY53 (OsWRKY53SD) showed further-enhanced disease resistance to the blast fungus compared to native OsWRKY53-overexpressing rice plants, and a substantial number of defense-related genes, including pathogenesis-related protein genes, were more upregulated in the OsWRKY53SD-overexpressing plants compared to the OsWRKY53-overexpressing plants. These results strongly suggest that the OsMKK4-OsMPK3/OsMPK6 cascade regulates transactivation activity of OsWRKY53, and overexpression of the phospho-mimic mutant of OsWRKY53 results in a major change to the rice transcriptome at steady state that leads to activation of a defense response against the blast fungus in rice plants.

show abstract

Section: Methodsmentioning

confidence: 99%

“…To examine fungal lesions and biomass, fungal inoculation of rice leaf blades was carried out as described previously [38] with some modifications. Fourteen-day-old rice plants were placed on moistened filter paper in plastic trays.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus

et al. 2013

View full text Add to dashboard Cite

WRKY transcription factors form a large family of plant-specific transcription factors and participate in plant defense responses either as positive or negative regulators. In this study, we comprehensively analyzed the role of one of the group IIa WRKY transcription factors in rice, OsWRKY28, in the regulation of basal defense responses to a compatible race of the rice blast fungus Magnaporthe oryzae, strain Ina86-137. The expression analyses of the group IIa WRKY transcription factors in rice revealed that OsWRKY28, together with OsWRKY71, exhibit an early-induced expression prior to the late-induced expressions of OsWRKY62 and OsWRKY76. The GFP-OsWRKY28 fusion protein localized mainly in the nuclei of onion epidermal cells, and the maltose-binding protein-fused OsWRKY28 recombinant protein specifically bound to W-box elements. A transient reporter gene assay clearly showed that OsWRKY28 functions as a transcriptional repressor. Overexpression of OsWRKY28 in rice plants resulted in enhanced susceptibility to Ina86-137. Finally, transcriptome analysis revealed that the induction of several defense-related genes in the wild type after Ina86-137 infection was counteracted in OsWRKY28-overexpressing rice plants. These results strongly suggest that OsWRKY28 is a negative regulator of basal defense responses against Ina86-137 and acts as a modulator to maintain the responses at an appropriate level by attenuating the activation of defense-related gene expression levels.

show abstract

Molecular and cellular analysis of the biotrophic interaction between rice and Magnaporthe oryzae – Exploring situations in which the blast fungus controls the infection

Mochizuki

Yokotani

Nishimura

et al. 2016

Physiological and Molecular Plant Pathology

View full text Add to dashboard Cite

Identification and Characterization of 2′-Deoxyuridine from the Supernatant of Conidial Suspensions of Rice Blast Fungus as an Infection-Promoting Factor in Rice Plants

Cited by 5 publications

References 47 publications

Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice

Overexpression of Phosphomimic Mutated OsWRKY53 Leads to Enhanced Blast Resistance in Rice

OsWRKY28, a PAMP-responsive transrepressor, negatively regulates innate immune responses in rice against rice blast fungus

Molecular and cellular analysis of the biotrophic interaction between rice and Magnaporthe oryzae – Exploring situations in which the blast fungus controls the infection

Contact Info

Product

Resources

About