Identification and characteristics of phenol degrading bacteria ad049 screened from oil contaminated soil

Abstract

A bacterium strain, which prove to be highly effective in phenol degrading, was isolated from soil contaminated with oil in Jingbian oilfield of Northern Shaanxi by using enrichment culture method with phenol as a sole carbon source.The bacterium was named ad049 and identified as Rhodococcus on the basis of the morphological characterizations, physiological and biochemical identification, and alignment analysis of the 16S rDNA sequence. Effects of inoculation quantity of ad049, pH, temperature, and concentration of phenol as a substrate on bacterial growth and the efficiency of ad049 on phenol degradation were also investigated by shaker culture method, and dehydrogenase and catechol dioxygenase were determined as well. Results showed that the ad049 had a strong biodegradation capability for phenol. It degraded 99% of the phenol in a concentration of 1000 mg / L in 24 hours under the incubating condition of 30 益 , pH 8, and 5% of ad049 inoculation. The degradation process could be described with zero order kinetics equation with the k 0 and R 2 values of 41郾 51and 0.96, respectively. The degradation of phenol by ad049 might be achieved largely by 1,2鄄dioxygenase for ortho ring opening of benzene, and partly by catechol 2,3鄄dioxygenase for meta鄄cleavage.

A new Rhodococcus aetherivorans strain isolated from lubricant-contaminated soil as a prospective phenol-biodegrading agent

“…The variable ability to assimilate phenol has been detected for several different species of genus Rhodococcus: R. coprophilus (Nagamani and Lowry 2009), R. erythropolis (Soudi and Kolahchi 2011; Korobov et al 2017;Jha and Veena 2017), R. gordoniae (Mishra and Lal 2014), R. jostii (Zidkova et al 2010), R. opacus (Shumkova et al 2009;Yoneda et al 2016), R. phenolicus (Rehfuss and Urban 2005), R. pyridinivorans (Kumari et al 2013;Al-Defiery and Gopal 2015;Al-Defiery and Reddy 2013;Moghadam et al 2016), R. rhodnii (Solyanikova and Golovleva 2015), R. rhodochrous (Przybulewska et al 2006), R. ruber (Pannier et al 2012) as well as other unidentified strains of Rhodococcus sp. (Suhaila et al 2010(Suhaila et al , 2013Arif et al 2012;Paisio et al 2012;Hu et al 2014;Margesin et al 2005;Nawawi et al 2017). Evaluation of phenol degradation in such studies was carried out under different varying conditions and with initial phenol concentrations ranging from 100 to 200 up to 2000 mg L −1 .…”