Identification and characteristics of microRNAs from Bombyx mori

He, Ping‐an; Nie, Zhou; Chen, Jianqing; Chen, Jian; Lv, Zhengbing; Sheng, Qing; Zhou, S. P.; Gao, Xiaolian; Kong, Lingyin; Wu, Xiang-Fu; Jin, Yongfeng; Zhang, Yaozhou

doi:10.1186/1471-2164-9-248

Cited by 65 publications

(54 citation statements)

References 59 publications

(57 reference statements)

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“…2E, the frequency of the four nucleotides (A, C, G and U) differed at each position. Previous studies have shown that animal mature miRNAs use U residues most frequently at their 5 0 end [33,34]. Our analysis of sequence characteristics at each position suggested that this trend is obvious for most of human mature miRNA sequences.…”

Section: Sequence Characteristics Of Different Lineage-specific Humansupporting

confidence: 53%

Systematic analysis of human microRNA divergence based on evolutionary emergence

et al. 2010

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a b s t r a c tMicroRNAs (miRNAs) play important roles in post-transcriptional gene expression control. To gain new insight into human miRNAs, we performed comprehensive sequence-based homology search for known human miRNAs to study the evolutionary distribution of human miRNAs. Furthermore, we carried out a series of studies to compare various features for different lineage-specific human miRNAs. Our results showed that major expansions of human miRNA genes coincide with the advent of vertebrates, mammals and primates. Further system-level analysis revealed significant differences in human miRNAs that arose from different evolutionary time points for a number of characteristics, implicating genetic and functional diversification for different human miRNAs during evolution. Our finds provide more useful knowledge for further exploring origins and evolution of human miRNA genes.

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Section: Sequence Characteristics Of Different Lineage-specific Humansupporting

confidence: 53%

Systematic analysis of human microRNA divergence based on evolutionary emergence

et al. 2010

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“…[21][22][23][24] More than 487 miRNAs have been identified in B. mori (miRBase release 21, June 2014), but their functions are only beginning to be understood. We show here that miR-2 and their target genes, abnormal wing disc (Bmawd) and fringe (Bmfng), mediate wing morphogenesis in this lepidopteron model insect.…”

Section: Introductionmentioning

confidence: 99%

MiR-2 family targetsawdandfngto regulate wing morphogenesis inBombyx mori

Lin

et al. 2015

RNA Biology

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(2015) MiR-2 family targets awd and fng to regulate wing morphogenesis in Bombyxmori, RNA Biology, 12:7, 742-748, DOI: 10.1080/15476286.2015 Keywords: CRISPR/Cas9, Gal4/UAS, miR-2 cluster, transgene, wing disc MicroRNAs (miRNAs) are post-transcriptional regulators that target specific mRNAs for repression and thus play key roles in many biological processes, including insect wing morphogenesis. miR-2 is an invertebrate-specific miRNA family that has been predicted in the fruit fly, Drosophila melanogaster, to be involved in regulating the Notch signaling pathway. We show here that miR-2 plays a critical role in wing morphogenesis in the silkworm, Bombyx mori, a lepidopteran model insect. Transgenic over-expression of a miR-2 cluster using a Gal4/UAS system results in deformed adult wings, supporting the conclusion that miR-2 regulates functions essential for normal wing morphogenesis. Two genes, abnormal wing disc (awd) and fringe (fng), which are positive regulators in Notch signaling, are identified as miR-2 targets and validated by a dual-luciferase reporter assay. The relative abundance of both awd and fng expression products was reduced significantly in transgenic animals, implicating them in the abnormal wing phenotype. Furthermore, somatic mutagenesis analysis of awd and fng using the CRISPR/Cas9 system and knock-out mutants also resulted in deformed wings similar to those observed in the miR-2 overexpression transgenic animals. The critical role of miR-2 in Bombyx wing morphogenesis may provide a potential target in future lepidopteran pest control.

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“…Moreover, the silkworm genome has been reported in 2004, which provides opportunities for a thorough research for functional genomics (Xia et al, 2004). Because of the important functions of miRNA for gene regulation, our group firstly large-scale identified miRNA genes in Bombyx mori (He et al, 2008). Since then, due to the development of deep sequencing technology, a large number of miRNAs have been identified in silkworm (Cai et al, 2010;Huang et al, 2010;Liu et al, 2010b;Yu et al, 2008Yu et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%