Identification and characterisation of multiple glutathione <i>S</i>‐transferase genes from the diamondback moth, <i>Plutella xylostella</i>

Chen, Xien; Zhang, Yalin

doi:10.1002/ps.3884

Cited by 38 publications

(30 citation statements)

References 65 publications

(132 reference statements)

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“…The K m value of CpGSTe2 toward CDNB was much lower than for other recombinant enzymes, suggesting a higher affinity of CpGSTe2 toward the substrate (CDNB) than is found in other proteins. The V max value for CpGSTe3 against CDNB was higher than for CpGSTe2 and CpGSTd4, similar to the V max values reported in AgGSTd2 in A. gambiae , PxGSTe3 in P. xylostella , and CpGSTd2 in C. pomonella , but was much lower than those of CpGSTd1 in C. pomonella . The difference in kinetic properties between delta and epsilon GSTs in C. pomonella may be related to the catalytic capacity of various GSTs in C. pomonella .…”

Section: Discussionsupporting

confidence: 79%

“…In B. dorsalis , the expressions of multiple GST genes were increased by β‐cypermethrin, suggesting that the GST gene may function in detoxifying β‐cypermethrin . In contrast, the mRNA transcript of the remaining GST gene CpGSTe1 was decreased after treatment with a LD 10 of λ ‐cyhalothrin for 3–12 h. Down‐regulated expression of GST genes has been also reported in P. xylostella , N. lugens , C. medinalis , and L. decemlineata after exposure to insecticides . Down‐regulation of a subset of GSTs may be an adaptive mechanism in insects for reducing the total enzymatic activity of GST enzymes to prevent exhausting the supply of GSH .…”

Section: Discussionmentioning

confidence: 89%

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Functional characterization of a novel λ‐cyhalothrin metabolizing glutathione S‐transferase, CpGSTe3, from the codling moth Cydia pomonella

Wang

et al. 2019

Pest Management Science

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BACKGROUND Recent work has shown that two codling moth (Cydia pomonella) glutathione S‐transferase genes (GSTs), CpGSTd1 and CpGSTd3, can metabolize λ‐cyhalothrin, one of the recommended insecticides for C. pomonella control worldwide. However, systematical characterization of delta and epsilon GSTs, especially their potential contributions in the metabolism of λ‐cyhalothrin, is currently still lacking in C. pomonella. RESULTS In this study, a total of nine cDNA sequences were identified in C. pomonella, including four in the delta and five in the epsilon subclasses. RT‐qPCR showed that seven GSTs were ubiquitously expressed at all developmental stages, and CpGSTe2, CpGSTe3, and CpGSTe4 were mainly expressed in larvae. The mRNA levels of CpGSTd2, CpGSTd4, and CpGSTe5 were significantly higher in male than in female adults. Tissue‐specific expression analysis revealed that the CpGSTe2, CpGSTe3, and CpGSTe4 were highly expressed in the midgut while CpGSTd2 and CpGSTd4 were predominantly expressed in the Malpighian tubules. The transcripts of these GSTs (except CpGSTe1) were co‐expressed following exposure to LD10 of λ‐cyhalothrin for 3 h. Recombinant CpGSTd4, CpGSTe2, and CpGSTe3 proteins expressed in Escherichia coli displayed glutathione‐conjugating activity toward 1‐chloro‐2,4‐dinitrobenzene. In addition, λ‐cyhalothrin could inhibit the activity of recombinant CpGSTd4, CpGSTe2, and CpGSTe3 enzymes, but only recombinant CpGSTe3 showed λ‐cyhalothrin metabolic capacity, with 21.88 ± 1.09% of parental compound being depleted within 1 h. CONCLUSION These data show that CpGSTe3 is a third GST gene, encoding an enzyme that metabolizes λ‐cyhalothrin in C. pomonella. © 2019 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 89%

Functional characterization of a novel λ‐cyhalothrin metabolizing glutathione S‐transferase, CpGSTe3, from the codling moth Cydia pomonella

Wang

et al. 2019

Pest Management Science

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“…Three members of the cytochrome P450 family (XM_011553684, XM_011550453 and XM_011567156) and GST (XM_011563733), which might be involved in detoxification pathways (Baek et al ., ; Chen & Zhang, ), were predicted as potential target genes of the miRNAs miR‐2b‐3p, miR‐14b‐5p and let‐7‐5p. Cytochrome P450 9f2 ( CYP9F2 ) harbours a binding site for miR‐2b‐3p in its 3' UTR whereas the binding sites of the other two miRNAs are located in the coding regions of their target genes (Fig.…”

Section: Resultsmentioning

confidence: 99%

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

Etebari

Afrad

Tang

et al. 2018

Insect Molecular Biology

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The diamondback moth, Plutella xylostella, has developed extremely high levels of resistance to chlorantraniliprole and other classes of insecticides in the field. As microRNAs (miRNAs) play important roles in various biological processes through gene regulation, we examined the miRNA profile of P. xylostella in response to chlorantraniliprole exposure. RNA sequencing analysis showed that insecticide treatment caused significant changes in the abundance of some miRNAs. Increasing exposure time and insecticide concentration induced more dysregulated miRNAs in P. xylostella larvae. We also screened potential target genes for some of the differentially expressed miRNAs (such as miR-2b-3p, miR-14b-5p and let-7-5p), which may play important roles in insecticide resistance development. Exposure of P. xylostella larvae to chlorantraniliprole caused considerable overexpression in the transcript levels of potential target genes cytochrome P450 9f2 (CYP9F2) and 307a1 (CYP307a1). Application of miR-2b-3p and miR-14b-5p mimics significantly suppressed the relative transcript levels of CYP9F2 and CYP307a1, respectively, in a P. xylostella cell line. Furthermore, enrichment of P. xylostella diet with miR-2b-3p mimics significantly increased mortality in deltamethrin-resistant larvae when exposed to deltamethrin. The results suggest that miR-2b-3p may suppress CYP9F2 transcript levels in P. xylostella and consequently inhibit larval detoxification pathways. The findings provide an insight into possible role of miRNAs in regulation of metabolic resistance of insects to insecticides.

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“…For example, the relative expression levels of P450 and GST family genes in different developmental stages of P. xylostella have been investigated recently by Yu et al . and Chen and Zhang, respectively. When the expression fold changes were considered, the 21 UGT mRNAs showed dramatic differences in the same stage with ranges of 1.0–584‐fold (eggs), 2.6–1820‐fold (larvae), 7.6–580‐fold (pupae) and 2.1–1920‐fold (adults) (Table S3).…”

Section: Resultsmentioning

confidence: 99%

Characterization of UDP‐glucuronosyltransferase genes and their possible roles in multi‐insecticide resistance in Plutella xylostella (L.)

Shi

Gao

et al. 2017

Pest Management Science

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Identification and characterisation of multiple glutathione S‐transferase genes from the diamondback moth, Plutella xylostella

Abstract: The changes in DBM GST gene expression levels exposed to different insecticides indicate that they may play individual roles in tolerance to insecticides and xenobiotics.

Cited by 38 publications

References 65 publications

Functional characterization of a novel λ‐cyhalothrin metabolizing glutathione S‐transferase, CpGSTe3, from the codling moth Cydia pomonella

Functional characterization of a novel λ‐cyhalothrin metabolizing glutathione S‐transferase, CpGSTe3, from the codling moth Cydia pomonella

Involvement of microRNA miR‐2b‐3p in regulation of metabolic resistance to insecticides in Plutella xylostella

Characterization of UDP‐glucuronosyltransferase genes and their possible roles in multi‐insecticide resistance in Plutella xylostella (L.)

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