Identification and characterisation of Emp53, the homologue of human tumor suppressor p53, from Echinococcus multilocularis: its role in apoptosis and the oxidative stress response

Cheng, Zhe; Zhu, Shan; Wang, Liang; Liu, Fan; Tian, Huimin; Pengsakul, Theerakamol; Wang, Yanhai

doi:10.1016/j.ijpara.2015.02.010

Cited by 14 publications

(13 citation statements)

References 50 publications

(26 reference statements)

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“…[ 31 , 32 ]. Human tumor suppressor p53 was effected by UA, and its homologue-Emp53 in E. multilocularis was reported to play a functional role in the E. multilocularis metacestode [ 33 ]. In addition to the induction of apoptosis, UA has also been reported to prevent the metastasis of cancer through down-expression of matrix metalloproteinases (MMP-2 and MMP-9) [ 12 , 34 ] relating to the microenvironment of cancer cells; these enzymes were detected in E. granulosus hydatid cyst fluid, cyst membranes and protoscolices, and are involved in pathology-related tissue remodeling [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of ursolic acid against Echinococcus granulosus in vitro and in a murine infection model

et al. 2018

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BackgroundCystic echinococcosis is a global public health problem; however, the drugs (albendazole and mebendazole) currently recommended by WHO for its treatment, have limited efficacy. Therefore, novel drugs are required to provide more choices for the treatment of this disease.MethodsThe anthelmintic effects of ursolic acid (UA) were tested on Echinococcus granulosus protoscoleces, germinal cells and metacestodes in vitro. The in vivo efficacy of UA was investigated in mice following secondary infection with E. granulosus. Furthermore, the corresponding ultrastructural damage induced by UA was evaluated by electron microscopy.ResultsIn vitro, 45.95 ± 5.30% of protoscoleces were killed by UA at 40 μg/ml, while the growth of more than 90% of germinal cells was inhibited by UA at 10 to 40 μg/ml. The same effect was observed in metacestodes 7 days after treatment with UA at 10, 20 and 40 μg/ml, and more than 50% of metacestodes showed loss of integrity at the end of the experiment. In vivo, metacestode weight was significantly reduced following oral administration of UA at 200 and 100 mg/kg (39.5 and 38.3%, respectively). Additionally, ultrastructural damage, such as alternations in germinal cell morphology and formation of vacuoles and lipid granules were observed in parasites treated with UA in vitro, while detachment of the germinal layer from the laminated layer was also seen in metacestodes in vivo.ConclusionsUA was demonstrated to exert parasiticidal activity against E. granulosus in vitro and in vivo, thus implicating UA as a potential anti-echinococcosis agent.

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Section: Discussionmentioning

confidence: 99%

Efficacy of ursolic acid against Echinococcus granulosus in vitro and in a murine infection model

et al. 2018

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“…Noya et al (2014) ½Q14 also demonstrated that mucinlike peptides from E. granulosus induce antitumour activity. In studies on the identification and characterization of Emp53, the homologue of human tumour suppressor p53, from E. multilocularis, Cheng et al (2015) concluded that since the parasite develops in host organs, it must have evolved a stress defence system, which could involve Emp53, to cope with various genotoxic and cellular stresses that may cause DNA damage and genomic instability. p0240…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%

Biology and Systematics of Echinococcus

Thompson

2017

Advances in Parasitology

305

282

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“…However, there are few studies on the function of CaM in parasites dealing with abiotic stress. During development and proliferation in the host, E. granulosus must cope with oxidants and reactive oxygen species (ROS) derived from their own metabolism and from the host’s activated immune cells [ 34 ]. To further study the function of CaM in E. granulosus under the oxidative stress, PSCs were treated with 5 mM H 2 O 2 for the indicated times up to 6 h at 37 °C.…”

Section: Discussionmentioning

confidence: 99%

Molecular and biochemical characterization of calmodulin from Echinococcus granulosus

et al. 2017

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Background Echinococcus granulosus is a harmful cestode parasite that causes cystic echinococcosis in humans as well as various livestock species and wild animals. Calmodulin (CaM), a Ca2+ sensor protein, is widely expressed in eukaryotes and mediates a variety of cellular signaling activities.MethodsIn the present study, the cDNA encoding CaM in Echinococcus granulosus (rEgCaM) was successfully cloned and the molecular and biochemical characterizations carried out. The antigenicity and immunoreactivity of rEgCaM was detected and the preliminary enzyme-linked immunosorbent assay (ELISA)-based serodiagnostic potential of EgCaM was assessed. The locations of this protein in the adult worm and larval stage, and the mRNA expression in different states of E. granulosus protoscoleces (PSCs) were defined clearly. Moreover, the Ca2+-binding properties of EgCaM were measured.ResultsrEgCaM is a highly conserved calcium-binding protein, consisting of 149 amino acids. Immunoblotting analysis revealed that rEgCaM could be identified using E. granulosus infected sheep serum. The use of rEgCaM as an antigen was evaluated by indirect ELISA which exhibited a high sensitivity (90.3%), but low specificity (47.1%). rEgCaM was ubiquitously expressed in protoscoleces and adults of E. granulosus, as well as in the germinal layer of the cyst wall. The mRNA expression level of rEgCaM was increased from the start of H2O2 exposure and then gradually decreased because of the increased apoptosis of PSCs. In electrophoretic mobility tests and 1-anilinonaphthalene-8-sulfonic acid assays, rEgCaM showed a typical characteristic of a calcium-binding protein.ConclusionsTo our knowledge, this is the first report on CaM from E. granulosus and rEgCaM is likely to be involved in some important biological function of E. granulosus as a calcium-binding protein.

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Identification and characterisation of Emp53, the homologue of human tumor suppressor p53, from Echinococcus multilocularis: its role in apoptosis and the oxidative stress response

Cited by 14 publications

References 50 publications

Efficacy of ursolic acid against Echinococcus granulosus in vitro and in a murine infection model

Efficacy of ursolic acid against Echinococcus granulosus in vitro and in a murine infection model

Biology and Systematics of Echinococcus

Molecular and biochemical characterization of calmodulin from Echinococcus granulosus

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