Identification and characterisation of early reactions of asparagine-linked oligosaccharide assembly in Entamoeba histolytica

Biosynthesis of glycoproteins in the pathogenic fungusCandida albicans: Activation of dolichol phosphate mannose synthase by cAMP-mediated protein phosphorylation

“…Optimal stimulation was observed at PE/PI ratios of 2.5–5.0 and this was not substantially higher than that attained with the individual phospholipids. In terms of activation by PE, Candida DPMS is reminiscent of that from mammalian [5], yeast [22] and protozoan [7] cells, suggesting that this phosphoglyceride, and also PI in the case of Candida , may be a critical component of the enzyme hydrophobic environment in intact membranes. Activity of solubilized DPMS, on the other hand, did not depend on exogenous phospholipids suggesting that these were solubilized along with the enzyme (not shown).…”

“…Activity of solubilized glycosyltransferases is usually restored by phospholipids bearing distinct polar heads [5,7,[20][21][22]. Here, although some activity was observed in the absence of added phospholipids, this was largely stimulated by PI and to a lower extent by phosphatidylethanolamine (PE) at optimal concentrations of 6.25 and 12.5 lg per assay, respectively.…”