Identification and Characterisation of Bacteria Causing Soft-rot in Agave tequilana

Jiménez-Hidalgo, Isadora; Virgen-Calleros, Gil; Vega, Octavio Martínez-de la; Vandemark, George J.; Olalde‐Portugal, Víctor

doi:10.1023/b:ejpp.0000019791.81935.6d

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…: utilization of D-fructose, D-galactose, a-D-glucose, D-mannitol, D-mannose, sucrose, D-trehalose, glycerol and gentibiose but not of D-raffinose, glycogen, propionic acid, L-threonine and ketoglutaric acid, as carbon sources. In contrast with the metabolic profile reported for E. cacticida (Jiménez- Hidalgo et al 2004), this strain of Pectobacterium can utilize D-sorbitol, acetic acid and formic acid as carbon sources. Moreover, P. cacticidum FHLGJ22 was able to grow at 43°C and yielded negative results when subjected to the H 2 S, urea, ornithine decarboxylase and NO 2 tests.…”

Section: Biochemical Characterization Of P Cacticidumcontrasting

confidence: 89%

“…The black spots observed in softened-tissue areas were presumably caused by PEL activity, whose secretion was detected in vitro. Moreover, the lesions produced in agave were similar to the symptoms previously reported in agave plants infected with E. cacticida (Jiménez-Hidalgo et al 2004).…”

Section: Tissue Maceration Of Agave Bulbils By P Cacticidumsupporting

confidence: 84%

“…This was performed using the Biolog GN2 MicroPlates system (BIOLOG Inc., Hayward, CA, USA) as described previously (Jiménez-Hidalgo et al 2004). Bacteria were multiplied in the Biolog universal growth medium and incubated at 28°C.…”

Section: Metabolic Profiling Analysismentioning

confidence: 99%

“…This novel Pectobacterium strain was also found to cause severe soft rot and chlorosis, not only in this crop but in representative species of several other angiosperm families. Sequence data of the 16S ribosomal DNA gene and the intergenic transcribed spacer region (ITS), complemented with biochemical and microbiological analysis identified this bacterial pathogen as a novel strain of P. cacticidum, which has been previously reported to infect cacti and agave (Alcorn et al 1991;Jiménez-Hidalgo et al 2004). In addition, the finding of a bacterium that causes soft-rot and chlorosis of sunflower and other angiosperm plants complements the relatively few reports of similarly acting pathogenic bacteria (Ishiga et al 2009;Slawiak and Lojkowska 2009).…”

Section: Introductionmentioning

confidence: 99%

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Infection by a coronatine-producing strain of Pectobacterium cacticidum isolated from sunflower plants in Mexico is characterized by soft rot and chlorosis

Valenzuela-Soto¹,

Maldonado-Bonilla

Hernández-Guzmán

et al. 2015

J Gen Plant Pathol

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A novel strain of Pectobacterium, isolated from infected sunflower plants (Helianthus annuus L.) in Mexico was characterized. Inoculated sunflower plants developed both tissue chlorosis and soft-rot on leaves. The broad host range shown by this pathogen, which included members of the Agavaceae, Asteraceae, Brassicaceae and Solanaceae, was characteristic of the genus Pectobacterium. The metabolic profile and molecular data, in addition to the secretion of plant-cell-wall-degrading enzymes, confirmed its identification as a member of the Pectobacterium genus. A phylogenetic tree constructed on the basis of its 16S rDNA sequence revealed a high identity (98 %) with P. cacticidum. Amplification and restrictionfragment-length-polymorphism analysis of internal transcribed sequences further confirmed its classification as P. cacticidum. Similar to strains of P. atrosepticum, P. carotovorum and Dickeya spp., the new P. cacticidum strain FHLGJ22 has a coronafacic ligase gene and produces coronatine, a virulence factor usually associated with phytopathogenic strains of Pseudomonas syringae. Our results suggest that this strain may utilize a dual infection process involving cell maceration and plant toxins, which is synchronized via an unknown mechanism, might confer an adaptive advantage to colonize different plant hosts.

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Section: Biochemical Characterization Of P Cacticidumcontrasting

confidence: 89%

Section: Tissue Maceration Of Agave Bulbils By P Cacticidumsupporting

confidence: 84%

Section: Metabolic Profiling Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Infection by a coronatine-producing strain of Pectobacterium cacticidum isolated from sunflower plants in Mexico is characterized by soft rot and chlorosis

Valenzuela-Soto¹,

Maldonado-Bonilla

Hernández-Guzmán

et al. 2015

J Gen Plant Pathol

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show abstract

“…During the past few years, the bacterium Erwinia carotovora and the fungus Fusarium oxysporum have been causing severe damage to A. tequilana plantations in México, including the states of Guanajuato, Jalisco, Michoacán, Nayarit, and Tamaulipas (Jiménez-Hidalgo et al 2004;Ávila-Miranda et al 2010). Since the 1990s, this species has been severely attacked by diverse diseases caused by bacteria and fungi, and exposed to a multitude of natural abiotic stressors, which reduce both quality and yield of fermentable juices.…”

Section: Agave Tequilanamentioning

confidence: 99%