Identification and bioinformatic analysis of Aux/IAA family based on transcriptome data of Bletilla striata

Abstract

Auxin/Indole-3-Acetic Acid (Aux/IAA) genes are involved in auxin signaling pathway and play an important role in plant growth and development. However, many studies focus on Aux/IAA gene families and much less known in Bletilla striata. In this study, a total of 27 Aux/IAA genes (BsIAA1-27) were cloned from the transcriptome of Bletilla striata. Based on a phylogenetic analysis of the Aux/IAA protein sequences from B. striata, Arabidopsis thaliana and Dendrobium officinale, the Aux/IAA genes of B. striata (BsIAAs) were categorized into 2 subfamilies and 9 groups. While BsIAAs were more closer to those of D. officinale compared to A. thaliana. EST-SSR marker mining test showed that 4 markers could be stably amplified with obvious polymorphisms among 4 landraces. Our results suggested that BsIAAs were involved in the process of tuber development and provided insights into functional roles of Aux/IAA genes in B. striata and other plants.In this paper, we identified the 27 members of the Aux/IAA gene family which can be divided into 2 groups and 9 subfamilies. The similar branches of Aux/IAA proteins had the same or similar motifs, such as genes between BsIAA10 and BsIAA12, BsIAA11 and BsIAA13, BsIAA18 and BsIAA20, indicating that Aux/IAA proteins were conserved. Among them, BsIAAs were more closer to those of D. officinale compared to A. thaliana.

Regulatory Mechanisms of ArAux/IAA13 and ArAux/IAA16 in the Rooting Process of Acer rubrum

“…As the grafting propagation process is tedious, time-consuming, and labor-intensive, it is necessary to select and purchase high-affinity rootstocks with scions. Later management also requires a lot of labor and material resources [4] (Li et al, 2019), and so compared with grafting propagation, cutting propagation is more effective in propagating A. rubrum in China at present.…”

Genome-wide identification of Aux/IAA and ARF gene families reveal their potential roles in flower opening of Dendrobium officinale

“…The genome-wide identification of the Aux/IAA gene family has been achieved in many monocotyledonous and dicotyledonous plant species, including Bletilla striata (27 members) [ 25 ], Dendrocalamus sinicus (26 members) [ 26 ], Hordeum vulgare (36 members) [ 27 ], Trichosanthes dioica (37 members) [ 28 ], Acer rubrum (17 members) [ 29 ] and Ricinus communis (19 members) [ 30 ]. In this study, we reported the first systematic analysis of the Aux/IAA and ARF gene family in the Dendrobium genus, and a total of 14 putative Aux/IAA and 26 ARF genes were validated from the D. officinale genome .…”

“…contrast, nine genes (DoARF1, 2, 10,16,17,18,21,22,23) displayed a decreasing trend from S1-S3 and exhibited a positive correlation (r > 0.7, p < 0.05) with IAA content. Another nine genes (DoARF7, 8,12,13,14,15,24,25,26) exhibited an unchanged or alternative trend (increasing from S1 to S2, decreasing from S2 to S3…”

Transcriptome Analysis Reveals the Molecular Mechanism of Grape Inflorescence Elongation after Applying Microbial Fertilizers

“…Auxin is an important hormone in the plant developmental process (Chen L et al 2019). Functional analyses of AUX/IAA family members have indicated that they play various roles during plant development, such as root development, shoot growth, and fruit ripening (Luo et al 2018), in many plants, including wheat (Qiao et al 2017), bletilla (Bletilla striata; Liu H et al 2019), dragon bamboo (Dendrocalamus sinicus; Chen L et al 2019), and arabidopsis (Lakehal et al 2019). However, the specific regulation mechanisms by which AUX interacts with inflorescence elongation in plants remain unclear.…”