Identification and behavior of the precipitating BLV antibodies in sera of leukotic cattle

Matthaeus, W.; Kaaden, Oskar‐Rüger; Frenzel, B.; Schlotterer, E.

doi:10.1002/ijc.2910220210

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…The reported heterogeneous molecular sizes of the BLV-precipitating antibodies (5) were confirmed by these studies. Further work will be necessary to study the occurrence of 4 S antibodies reacting with gp 60 in young cattle.…”

Section: Analysis Of Antigen Preparations and Serologically Related Asupporting

confidence: 74%

“…A mixture of BLV antigens, antigens gp60 (previously designated gp69) and p24, were separated from the clarified supernatant from BLV-infected fetal lamb kidney cells (5). Sucrose density gradient centrifugation and disruption of the BLV particles by Triton X-100 gave a mixture of BLV antigens in the supernatant following ultracentrifugation.…”

Section: Blv Antigensmentioning

confidence: 99%

“…The gp 60 was isolated from the clarified supernatant by ammonium sulphate precipitation and purified in two steps by affinity chromatography on matrix-coupled concanavalin A and ion-exchange on DEAE-cellulose. The preparation appeared to be immunoelectrophoretically homogeneous (4) but to show minor amounts of a serologically related antigen in the agar gel immunodiffusion test (5).…”

Section: Blv Antigensmentioning

confidence: 99%

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Detection of Precipitating Antibodies and Identification of Bovine Leukemia Virus Antigens by Micro Counter-Current Immunoelectrophoresis

Matthaeus¹,

Straub²

2010

Zentralblatt Für Veterinärmedizin Reihe B

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IntroductionFor the diagnosis of bovine leukosis the agar gel immunodiffusion test is preferentially used in many countries (reviewed in: 9), although its technical conditions have not been standardized yet. RHODE et al. (10) studied the nature of bovine and ovine leukemia viral antigens involved in the two precipitates of this test and confirmed previous results (2) according to which the major internal protein (p 24) of bovine leukemia virus (BLV) is one of the precipitating agents. More recently, BEX et al. (1) compared the agar gel immunodiffusion test with radioimmunoassay in this respect.

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Section: Analysis Of Antigen Preparations and Serologically Related Asupporting

confidence: 74%

Section: Blv Antigensmentioning

confidence: 99%

Section: Blv Antigensmentioning

confidence: 99%

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Detection of Precipitating Antibodies and Identification of Bovine Leukemia Virus Antigens by Micro Counter-Current Immunoelectrophoresis

Matthaeus¹,

Straub²

2010

Zentralblatt Für Veterinärmedizin Reihe B

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“…Since AGID using BLV gp-51 antigen measures IgG exclusively (Matthaeus et al . , 1978) , decay of colostral BLV antibodies is essentially the decay of IgG-, • The half-life of IgG, in the bovine may vary from 18 to 22.7 days (Brar et al .…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of natural transmission of bovine leukemia virus infection / by Mark Cy Thurmond.

Thurmond¹

1982

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A 27-month study examined 473 dairy cattle for associations between bovine leukemia virus (BLV) infection and host and environmental factors. Cattle sera were tested at monthly intervals for BLV antibodies by agar-gel immunodiffusion using the glycoprotein-51 antigen. A model of BLV colostral antibody decay in 130 calves predicted infection in calves less than six months of age and estimated antibody half-life to be 27.1 ±1.2 days. Colostral antibody decay did not differ between BLV-infected and noninfected calves for slope (p = 0.45) or intercept (p = 0.43). By 95 days of age, 50% of the calves had no detectable BLV colostral antibodies. Of 125 calves born to BLV-infected cows and followed for at least four months, eight (6.4%) had precolostral BLV antibodies, as determined by radioimmunoassay using the glycoprotein-51 antigen-In utero infection with BLV was not associated with dam age (p = 0.86), dam parity (p = 0.83), breed (p = 0.66), sex (p = 0.11), or stage of gestation in which the dam was infected (p=0.50). Calves infected in utero did not pose an increased risk of infection to calves penned next to them (p = 0.61). Prevalence rates of infection were 9%, 16%, and 63% at 6, 16, and 27 months of age, respectively. Age-specific rates of infection were not associated with dam age (p = 0.79), dam parity (p=0.75), dam BLV-status (p = 0.46), breed (p = 0.86), or BLV-status of colostrum consumed (p=0.50). An algorithm was described which allocated probabilities of infection to locations occupied by an animal prior to detection of infection. Small calf pastures were associated with less infection than was the calf barn (p< 0.05). No less infection was associated with individual outdoor calf pens compared to contiguous indoor pens (p>0.05). Risk of infection associated with the dry herd was five times that for heifer pastures (p< 0.0001) and accounted 24 infections per 100 heifers per 100 days at risk. Vaccination for infectious diseases was not associated with increased BLV infection (p=0.33). Infection rates were not associated with month of birth (p = 0.24) or with season of potential arthropod vectors (p = 0.20). Heifer infection was likely to occur in late v/inter or spring (p =. 01). Interacting Computing (MUSIC) version 4.1, 197 8.

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“…Partículas virais foram encontradas no colostro e no leite de fêmeas infectadas (BUEHRING et al, 1994), corroborando com a sugestão de que o vírus pode ser transmitido da mãe para o neonato através da amamentação (POLLARI et al, 1993;NAGY et al, 2006). Não obstante, não foi evidenciado o risco de transmissão aos embriões através de sêmen contaminado, quando observados os protocolos de produção de embriões aprovados internacionalmente (CHOI et al, 2002;WRATHALL et al, 2006 (VALIKHOV, 1976;MATTHAEUS et al, 1978;CUNHA et al, 1982) (KEEFE et al, 1997a,b;KABEYA et al, 1999;AMILLS et al, 2004).…”

Section: Introductionunclassified

Influência da leucose enzoótica bovina na atividade oxidativa de leucócitos

Azedo¹

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Destino. Ele abençoou-me colocando em meu caminho a mais afável, a mais benevolente e, acima de tudo, a mais querida Orientadora que eu jamais poderia aspirar. Ou merecer. Por seus ensinamentos, pelo exemplo de profissional prestativa e paciente, de mãe, filha e esposa dedicada, pelo exemplo de mulher e amiga, Prof a Dr a Alice Maria Melville Paiva Della Libera, mais do que sinceramente grato, a você este trabalho é dedicado... você mora no meu coração. À carinhosa equipe formada pelas Médicas Veterinárias Pós-Graduandas Maiara Garcia Blagitz, Camila Rocha Bastos e Bárbara Gabriela Soares Sanches, pelo Médico Veterinário Fernando Nogueira de Souza, e pela aluna de graduação e bolsista de iniciação científica Camila Freitas Batista, nossa "caçulinha". Pessoas tão heterogêneas e tão intensamente dedicadas. Sempre lhes serei grato... e fiel. Este trabalho é fruto da colaboração de cada um de vocês!! A minha casa, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, berço da minha formação, que me acolheu novamente e me ofereceu as melhores oportunidades.

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Identification and behavior of the precipitating BLV antibodies in sera of leukotic cattle

Cited by 9 publications

References 13 publications

Detection of Precipitating Antibodies and Identification of Bovine Leukemia Virus Antigens by Micro Counter-Current Immunoelectrophoresis

Detection of Precipitating Antibodies and Identification of Bovine Leukemia Virus Antigens by Micro Counter-Current Immunoelectrophoresis

Epidemiology of natural transmission of bovine leukemia virus infection / by Mark Cy Thurmond.

Influência da leucose enzoótica bovina na atividade oxidativa de leucócitos

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