Identification and Antimicrobial Resistance in Klebsiella spp. Isolates from Turkeys in Poland between 2019 and 2022

Abstract

One of the important problems in poultry production is bacterial infections and increasing resistance to antibiotics. The increasing incidence of multidrug-resistant bacteria is a major challenge for physicians and veterinarians and considerably limits treatment options. This study was undertaken in order to assess the prevalence and antibiotic resistance of Klebsiella spp. strains isolated from turkeys sampled from 2019 to 2022 in Poland. The material for study consisted of clinical samples taken during routine monitoring and microbiological identification testing at commercial poultry farms. From all 507 isolates of Klebsiella, 95% were identified by MALDI-TOF (Matrix-Assisted Laser Desorption—Ionisation-Time of Flight) as Klebsiella pneumonia, 2% were Klebsiella oxytoca, 2% Klebsiella variicola, or unidentified (1%). All isolated Klebsiella strains were tested for antimicrobial susceptibility by disk diffusion. The results of our study indicated that colistin, neomycin, florfenicol and amoxicillin/clavulanic acid were the most effective against the Klebsiella spp. isolated from turkeys. In addition, the results show a decrease in the number of multi-resistant Klebsiella spp. strains between 2019 and 2021.

Microbiota Profiles of Hen Eggs from the Different Seasons and Different Sectors of Shanghai, China

“…Klebsiella spp. is an opportunistic Enterobacteriaceae that is highly pathogenic to poultry, livestock, and humans, and it is commonly found throughout China [25,26] as well as in other countries [27,28]. Here, Klebsiella contamination on the eggshell surfaces from the poultry farms was detected, with a season-dependent occurrence rate.…”

Limited evidence of spill over of antimicrobial resistantKlebsiella pneumoniaefrom animal/environmental reservoirs to humans in India

“…As reported previously, livestock isolates exhibited less abundance and diversity of acquired AMR genes, and the mechanisms of resistance were primarily directed against first-line drugs. 41,42 Based on these findings, it appears that AMR prevalence in different niches depends on a variety of site-specific conditions, such as antimicrobial use intensity and residual concentrations. 43 Similarly, livestock isolates contained significantly fewer virulence genes and mobile genetic elements than hospital-associated isolates.…”

Characterization of multidrug and heavy metal resistance of carbapenemases producing Klebsiella pneumoniae from poultry samples in Bangladesh

“…With an annual frequency of 10%-80%, bacterial poultry infections are among the most contagious illnesses poultry may get (Saha, Hoque, et al, 2020;Saha, Rakhi, et al, 2020). There has been little to no research done in Bangladesh (Hasan et al, 2012;Rahman et al, 2017Rahman et al, , 2018 on the heavy metal resistance of carbapenemases producing MDR KPN in poultry, despite the fact that the epidemiological prevalence of KPN and its antimicrobial resistance pattern has been studied at length in farm animals in other countries (Fielding et al, 2012;Kowalczyk et al, 2022). Therefore, in light of the current farming situation in Bangladesh, the purpose of this study was to characterize KPN isolates in poultry samples collected from chickens with poultry bacterial diseases with regard to their antimicrobial resistance and molecular identification of carbapenemases gene(s).…”