Identification and Antibacterial Activity of Lactobacillus Isolated from Different Raw Honey in Mostaganem Province, Algeria

Abstract

Background: The increase of multidrug-resistant pathogens causes increased concerns about the efficacy of conventional treatment, which requires the search for new alternatives to antibiotics. The present study aimed to evaluate the antibacterial activity of lactobacilli isolated from eight (n=8) typical Apis mellifera honey produced in Mostaganem region against four pathogenic bacteria responsible of bovine mastitis. Methods: Lacbacilli strains, isolated from different media were firstly presumptively identified by catalase test, Gram staining and cells morphology, than confirmed by partial 16S rRNA sequence analysis. Antibacterial activity of isolates was determined by the overlay agar method. Antibiotic susceptibility of mastitis pathogens was tested by disk diffusion technique. Result: Two species of lactobacilli were isolated and identified as Lactobacillus plantarum and Lactobacillus pentosus. Antibiotic sensitivity test demonstrated that mastitis pathogens showed the resistance to antibiotics tested. All the isolates showed antimicrobial activity with inhibition zone diameters ranged from 5±0.02 to 21±2.12 mm. The results revealed that honey can be considered as a source of Lactobacillus with antibacterial activities which can be used as alternative treatment of bovine mastitis.

Phytochemical Screening and Antibacterial Activity of Some Extracts from Atriplex halimus Growing in Algerian Sahara

“…The ethanol extracts from the aerial parts of A. halimus, containing alkaloids, steroids, flavonoids and glycosides, showed antibacterial activity against a variety of pathogenic gram-positive and gram-negative bacteria (Abdel Rahman et al, 2011). Endophytic fungi isolated from A. halimus has antibacterial effects against antibioticresistant bacterial species (Peláez et al, 1998;Dahou et al, 2021;Radja et al, 2022).…”

Identification of Indigenous Lactobacillus fermentum by 16S rRNA Gene Sequencing Isolated from Two Different Ecosystems: Newborn Infants and Bee’s Intestine

“…It is clear that the two strains of lactobacillus fermentum tested had a good inhibitory activity against the pathogenic germs Escherichia coli and Staphylococcus aureus with diameters of the inhibition zones ranging from 10 to 20 mm respectively. Radja et al (2022) obtained an antimicrobial activity with inhibition zone diameters ranged from 5±0.02 to 21±2.12 mm of Lactobacillus isolated from honey.…”