Identification and antagonistic activity in vitro of Bacillus spp. and Trichoderma spp. isolates against common phytopathogenic fungi

Rios-Velsaco, Claudio; Caro-Cisneros, Janeth; Reyes, David; Ruiz-Cisneros, María Fernanda; Ornelas‐Paz, José de Jesús; Salas‐Marina, Miguel Ángel; Villalobos-Pérez, Elizabeth; Prieto, Víctor Manuel Guerrero

doi:10.18781/r.mex.fit.1507-1

Cited by 19 publications

(31 citation statements)

References 18 publications

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“…The range of inhibition reported by several authors is broad, percentages of inhibition of 90 % are reported for B. subtilis strains against Fusarium sp. (Badía et al, 2011), and, on the other hand, percentages of inhibition of 29.4 % were reported for F. avenacum (Essghaier et al, 2012), and for B. methylotrophicus and B. amyloliquefaciens against F. oxysporum of 42.0 and 51.5 % (Rios et al, 2016); the latter are within the range obtained in this study. Strains CBMT2 and CBMT51 were the only ones to present halos of inhibition of mycelial growth in confrontation with F. equiseti with halos measuring 3.76 and 6.37 mm, respectively, which correspond to 18.3 and 31.5 % of inhibition ( Figure 1).…”

Section: Resultssupporting

confidence: 90%

“…The biotests were carried out by direct confrontation with the widely used dual planting (Li et al, 2011;Essghaier et al, 2012;Rios et al, 2016). In the center of 90 x 15 mm Petri dished with potato dextrose agar (PDA) medium, we placed a disc, 0.5 cm in diameter, of mycelium of the pathogen with 10 d of active growth, and innoculated 6 µL of a 1x10 8 UFC bacterial solution in four equidistant points around the mycelial growth at a distance of 2 cm.…”

Section: Inhibition Of Mycelial Growth In Vitro Ofmentioning

confidence: 99%

“…Los bioensayos se realizaron por confrontación directa con la técnica de cultivo dual ampliamente utilizada (Li et al, 2011;Essghaier et al, 2012;Rios et al, 2016); en cajas Petri de 90 x 15 mm con medio agar de papa y dextrosa (PDA), se colocó un disco de 0.5 cm de diámetro de micelio del fitopatógeno de crecimiento activo de 10 d en el centro de la caja y se inocularon 6 µL de una suspensión bacteriana de 1x10 8 UFC, en cuatro puntos equidistantes alrededor del crecimiento micelial a una distancia de 2 cm, las cajas se incubaron a 28 °C durante 7 d. Se midió el crecimiento radial y con los datos se calculó el porcentaje de inhibición micelial, tomando como testigo el crecimiento del hongo en PDA sin la presencia de bacterias.…”

Section: Inhibition Of Mycelial Growth In Vitro Ofunclassified

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Bacillus spp. en el Control de la Marchitez Causada por Fusarium spp. en Capsicum chinense

Mejía-Bautista¹,

Reyes-Ramírez²,

Cristóbal‐Alejo³

et al. 2016

RMF

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Resumen. El género Fusarium es un patógeno asociado al marchitamiento del chile y con reducción del rendimiento del cultivo. Las rizobacterias son una alternativa para mejorar la producción agrícola y protección contra fitopatógenos. En el presente estudio se evaluó el antagonismo in vitro de diez cepas de Bacillus contra Fusarium equiseti ITCF1 y F. solani ITCF2, todas la cepas bacterianas inhibieron el crecimiento micelial entre 21.28 y 71.70 %, adicionalmente las cepas CBMT2 y CBMT51 presentaron halos de inhibición contra F. equiseti con halos de 3.76 y 6.37 mm.

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Section: Resultssupporting

confidence: 90%

Section: Inhibition Of Mycelial Growth In Vitro Ofmentioning

confidence: 99%

Section: Inhibition Of Mycelial Growth In Vitro Ofunclassified

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Bacillus spp. en el Control de la Marchitez Causada por Fusarium spp. en Capsicum chinense

Mejía-Bautista¹,

Reyes-Ramírez²,

Cristóbal‐Alejo³

et al. 2016

RMF

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“…Aunque algunos fungicidas específicos han logrado reducir la severidad de la enfermedad en forma rápida y efectiva, su uso indiscriminado ha ocasionado la aparición de cepas resistentes y contaminación al ambiente (Qi et al, 2012). Por lo anteriormente señalado, en la actualidad para el manejo de Phytophthora se han considerado nuevas alternativas, basadas en el control biológico, estrategia importante en el manejo de fitopatógenos que habitan en el suelo, ya que reducen la aplicación de agroquímicos (Nguyen et al, 2012;Rios-Velasco et al, 2016).…”

Section: Resumen El Oomiceto Phytophthora Capsiciunclassified

“…Los mecanismos de control incluyen la producción de has led to the emergence of resistant isolates and environmental pollution (Qi et al, 2012). For this reason, new alternatives based on biological control are being considered for managing Phytophthora, an important strategy in soil-borne phytopathogen management, since they reduce the application of agrochemicals (Nguyen et al, 2012;Rios-Velasco et al, 2016). Different microorganisms have been reported to suppress P. capsici growth, including Streptomyces spp.…”

Section: Resumen El Oomiceto Phytophthora Capsicimentioning

confidence: 99%

Efecto de biocontrol e inhibición germinativa de Bacillus spp. sobre zoosporas de Phytophthora capsici

Ley-López

Márquez-Zequera

Carrillo-Fasio

et al. 2018

RMF

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Resumen. El Los cultivos de tomate (Solanum lycopersicum L.) y chile (Capsicum annuum L.) son de importancia económica por su consumo en la dieta humana. México se posiciona en el décimo lugar en producción de tomate con 2,649,358 t y segundo lugar en chile, con una producción de 2,294,400 t (SIAP 2016). Sin embargo, la producción de ambos cultivos es afectada por enfermedades ocasionadas por diferentes patógenos; entre éstos, destaca el oomiceto Phytophthora capsici Leonian, el cual es un microorganismo fitopatógeno que causa serios daños económicos en la agricultura en todo el mundo (Chen et al., 2016a). P. capsici ocasiona "damping-off", pudrición de raíz, daño en tallo, hoja o pudrición de frutos en más de 50 especies de plantas hospedantes (Erwin y Riveiro, 1996;Khan et al., 2011). Se ha reportado que este patógeno causa considerables pérdidas de rendimiento en B. thuringiensis the efficiency of biocontrol was 72 and 77% respectively, which show that these microorganisms can be used as biocontrol agents of P. capsici in tomato and chili plants.

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Characterization and control of Rhizoctonia solani affecting lucky bamboo (Dracaena sanderiana hort. ex. Mast.) using some bioagents

Abdel-Rahman

Abdel-Megeed

Salem

2023

Sci Rep

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In a survey conducted during the period of March–May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, and Giza governorates, Egypt), symptoms of root rot, basal stem rot, and wilt disease complex were observed in the lucky bamboo (Dracaena sanderiana hort. ex. Mast.). The highest disease infection percentage was found in lucky bamboo collected from Alexandria City (47.67%), while the highest disease severity was in lucky bamboo collected from El-Behera Governorate (35.19%). Rhizoctonia solani, Fusarium oxysporum, F. solani, Aspergillus niger, and Alternaria alternate were isolated and identified in the infected lucky bamboo samples. R. solani isolates were the most dominant among the recovered fungal species with a percentage of 80.89% of the total isolates (246). Pathogenicity tests showed that R. solani was the most pathogen with 100% disease infection and 76.67% disease severity. Molecular identification characterized R. solani isolate as R. solani AUMC 15120, MZ723906. Meanwhile, four biological control agents (bioagents) were isolated from the healthy lucky bamboo samples and identified based on cultural, morphological, microscopic characteristics, and the molecular phylogenetic analysis as Clonostachys rosea AUMC 15121, OL461708; Bacillus circulans TAG1, MW441316; B. siamensis TAP1, MW441318 and Ochrobactrum anthropi TAM1, MW441317. The four bioagents showed potential inhibition of R. solani in vitro as well as in vivo on lucky bamboo plants in vase treatments compared to the untreated inoculated control as well as certain fungicides and biocides used (Moncut, Rizolex-T, Topsin-M, Bio-Zeid, and Bio-Arc). The bioagent O. anthropi showed the highest inhibition growth (85.11%) of the in vitro R. solani colony, which was not significantly different from the biocide Bio-Arc (83.78%). However, C. rosea, B. siamensis and B. circulans showed inhibition values of 65.33, 64.44, and 60.44%, respectively. On the other hand, the biocide Bio-Zeid showed less inhibitory effect (43.11%), while the lowest growth inhibition was recorded by Rizolex-T (34.22%) and Topsin-M (28.67%). Furthermore, the in vivo experiment supported the in vitro results for the most effective treatments, where all the treatments significantly decreased the percentage of infection and disease severity compared to the inoculated untreated control. Additionally, the bioagent O. anthropi showed the highest effect, i.e., the lowest disease incidence and disease severity being 13.33% and 10%, compared to 100% and 75%, respectively, in the untreated inoculated control. This was not significantly different from the fungicide Moncut (13.33% and 21%) and from the bioagent C. rosea (20% and 15%) treatments for both parameters, respectively. In conclusion, the bioagents O. anthropi MW441317 at 1 × 108 CFU/ml as well as C. rosea AUMC15121 at 1 × 107/ml proved to be efficient to control R. solani causing root rot, and basal stem rot on lucky bamboo, compared to fungicide Moncut and can be used for disease management without the negative impact of the chemical control. Furthermore, this is the first report of the isolation and identification of Rhizoctonia solani, a pathogenic fungus, and four biocontrol agents (Bacillus circulans, B. siamensis, Ochrobactrum anthropi and Clonostachys rosea) associated with the healthy lucky bamboo plants.

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Identification and antagonistic activity in vitro of Bacillus spp. and Trichoderma spp. isolates against common phytopathogenic fungi

Abstract: Identification and antagonistic activity in vitro af

Cited by 19 publications

References 18 publications

Bacillus spp. en el Control de la Marchitez Causada por Fusarium spp. en Capsicum chinense

Bacillus spp. en el Control de la Marchitez Causada por Fusarium spp. en Capsicum chinense

Efecto de biocontrol e inhibición germinativa de Bacillus spp. sobre zoosporas de Phytophthora capsici

Characterization and control of Rhizoctonia solani affecting lucky bamboo (Dracaena sanderiana hort. ex. Mast.) using some bioagents

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