Identification and Analysis of Three Hub Prognostic Genes Related to Osteosarcoma Metastasis

Tan, Jianye; Liang, Huiying; Yang, Bo; Zhu, Shuang; Wu, Guofeng; Li, Lutao; Liu, Zhengwei; Li, Lin; Qi, Weizhong; Li, Sijing

doi:10.1155/2021/6646459

Cited by 9 publications

(10 citation statements)

References 36 publications

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“…RPL37A has been reported as a prognostic risk gene for osteosarcoma, which is consistent with the results of this study (10). MEF2C is necessary for the normal development of platelets and megakaryocytes and the (11). The results of this investigation further confirm this correlation.…”

Section: Discussionsupporting

confidence: 92%

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Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma

Zhang

Duan

et al. 2022

Front. Immunol.

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BackgroundTumor-infiltrating B cells play a crucial role in the promotion or inhibition of tumor development. However, the role of B cells in osteosarcoma remains largely unknown. The aim of this study was to investigate the effect of B cells on the prognosis and immunity infiltration of osteosarcoma.MethodsMarker genes of B cells were identified based on the single-cell sequencing results of osteosarcoma in the GEO database. The prognostic model was established by the TCGA database and verified by the GEO data. The divergence in immune infiltration between the low-risk and high-risk groups was then compared according to the established prognostic model. Finally, the differential genes in the low-risk and high-risk groups were enriched and analyzed.ResultsA total of 261 B cell marker genes was obtained by single-cell sequencing and a prognostic model of 4 B cell marker genes was established based on TCGA data. The model was found to have a good prediction performance in the TCGA and GEO data. A remarkable difference in immune infiltration between the low-risk and high-risk groups was also observed. The obtained results were verified by enrichment analysis.ConclusionIn summary, a prognostic model with good predictive performance was established that revealed the indispensable role of B cells in the development of osteosarcoma. This model also provides a predictive index and a novel therapeutic target for immunotherapy for clinical patients.

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Section: Discussionsupporting

confidence: 92%

“…The latest investigation by Tan et al. reported an indirect correlation between MEF2C and the adverse prognosis of osteosarcoma ( 11 ). The results of this investigation further confirm this correlation.…”

Section: Discussionmentioning

confidence: 99%

Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma

Zhang

Duan

et al. 2022

Front. Immunol.

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“…FOS also efficiently initiated key stem cell proliferation, migration, division, and differentiation [ 36 ]. MEF2C and FOS can regulate the prognostic genes related to Osteosarcoma Metastasis [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of genes contributing to cisplatin resistance in osteosarcoma cells

Xie

Dai

et al. 2022

FEBS Open Bio

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Osteosarcomas are prevalent in children and young adults and have a high recurrence rate. Cisplatin, doxorubicin, and methotrexate are common adjuvant chemotherapy drugs for treatment of osteosarcoma, but multidrug resistance is a growing problem. Therefore, understanding the molecular mechanisms of chemotherapy resistance in osteosarcoma cells is crucial for developing new therapeutic approaches and ultimately improving the prognosis of osteosarcoma patients. To identify genes associated with cisplatin resistance in osteosarcoma, we screened a large‐scale mutant library generated by transfecting human osteosarcoma cells with a piggyBac (PB) transposon‐based gene activation vector. Several candidate genes were identified by using Splinkerette‐PCR paired with Next Generation Sequencing. We created a disease‐free survival predictor model, which includes ZNF720 , REEP3 , CNNM2 , and CGREF1 , using TARGET (Therapeutically Applicable Research to Generate Effective Treatments) datasets. Additionally, the results of our enrichment analysis between the Four_genes_high group and Low_group suggested that these four genes may participate in cisplatin resistance in osteosarcoma through cross talk between various signaling pathways, especially the signaling pathway related to bone formation. These data may help guide future studies into chemotherapy for osteosarcoma.

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“…Based on the expression pattern and the tumor suppressor role of miR-1244 in OS, miR-1244-related mRNAs should exhibit high level and oncogenic role in OS. Among the predicted mRNA targets of miR-1244, we screened six mRNAs, including ABCG8 [35], ALDOA [36], RPL32 [37], PSMB2 [38], CEP55 [26], and PSMC2 [39]. After overexpressing miR-1244, the down-regulation of CEP55 mRNA was the most significant in both OS cell lines (Fig.…”

Section: Cep55 Is a Target Of Mir-1244 In Os Cellsmentioning

confidence: 99%

Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis

Gao

Miao

et al. 2022

J Orthop Surg Res

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Background: Accumulating articles demonstrate that circular RNAs play pivotal functions in tumorigenesis. However, the working mechanism of circ_0136666 in osteosarcoma (OS) progression remains to be further clarified. Methods:Real time-quantitative polymerase chain reaction and western blot assay were applied to determine RNA and protein expression, respectively. Cell proliferation was assessed by 5-Ethynyl-2′-deoxyuridine assay and colony formation assay. Transwell assays were carried out to assess cell migration and invasion abilities. Flow cytometry was performed to analyze cell apoptosis. Cell glycolysis was evaluated by analyzing the uptake of glucose and the production of lactate using the corresponding kits. Dual-luciferase reporter assay and biotinylated RNA-pull down assay were performed to confirm the target interaction between microRNA-1244 (miR-1244) and circ_0136666 or centrosomal protein 55 (CEP55). Xenograft tumor model was utilized to explore the role of circ_0136666 in tumor growth in vivo.Results: Circ_0136666 expression was prominently elevated in OS tissues and cell lines. Circ_0136666 absence restrained the proliferation, migration, invasion and glycolytic metabolism and promoted the apoptosis of OS cells. Circ_0136666 negatively regulated miR-1244 expression by binding to it in OS cells. MiR-1244 overexpression suppressed the malignant behaviors of OS cells. CEP55 was a target of miR-1244 in OS cells. Circ_0136666 positively regulated CEP55 expression partly by sequestering miR-1244 in OS cells. CEP55 overexpression largely reversed circ_0136666 silencing-mediated influences in OS cells. Circ_0136666 silencing significantly suppressed tumor growth in vivo. Conclusion:Circ_0136666 silencing inhibited OS progression partly by targeting miR-1244/CEP55 signaling. Silencing circ_0136666 and CEP55 or restoring miR-1244 level might be a potential therapeutic strategy for OS.

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Identification and Analysis of Three Hub Prognostic Genes Related to Osteosarcoma Metastasis

Cited by 9 publications

References 36 publications

Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma

Identification of B cell marker genes based on single-cell sequencing to establish a prognostic model and identify immune infiltration in osteosarcoma

Identification of genes contributing to cisplatin resistance in osteosarcoma cells

Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis

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