Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

Ibarra-Meneses, Ana Victoria; Amin, Atia; Dong, George; Olivier, Martin; Langlais, David; Fernández-Prada, Christopher

doi:10.1016/j.xpro.2023.102248

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Therefore, new methods are sought to detect and demonstrate the active presence of the parasite. Among these methods is the detection in biological fluids of products of the particulate secretome, especially exovesicles of the parasite, where both proteins and nucleic acids make it possible to demonstrate the presence of metabolically active forms of the parasite [37].…”

Section: Introductionmentioning

confidence: 99%

Use of sera cell free DNA (cfDNA) and exovesicle-DNA for the molecular diagnosis of chronic Chagas disease

Lozano,

Samblas,

Calabuig

et al. 2023

PLoS ONE

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Chagas disease, a neglected tropical disease, is now considered a worldwide health concern as a result of migratory movements from Central and South America to other regions that were considered free of the disease, and where the epidemiological risk is limited to transplacental transmission or blood or organ donations from infected persons. Parasite detection in chronically ill patients is restricted to serological tests that only determine infection by previous infection and not the presence of the parasite, especially in patients undergoing treatment evaluation or in newborns. We have evaluated the use of nucleic acids from both circulating exovesicles and cell-free DNA (cfDNA) from 50 samples twice randomly selected from a total of 448 serum samples from immunologically diagnosed patients in whom the presence of the parasite was confirmed by nested PCR on amplicons resulting from amplification with kinetoplastid DNA-specific primers 121F-122R. Six samples were randomly selected to quantify the limit of detection by qPCR in serum exovesicles. When the nucleic acids thus purified were assayed as a template and amplified with kinetoplastid DNA and nuclear satellite DNA primers, a 100% positivity rate was obtained for all positive samples assayed with kDNA-specific primers and 96% when SAT primers were used. However, isolation of cfDNA for Trypanosoma cruzi and amplification with SAT also showed 100% positivity. The results demonstrate that serum exovesicles contain DNA of mitochondrial and nuclear origin, which can be considered a mixed population of exovesicles of parasitic origin. The results obtained with serum samples prove that both cfDNA and Exovesicle DNA can be used to confirm parasitaemia in chronically ill patients or in samples where it is necessary to demonstrate the active presence of the parasite. The results confirm for the first time the existence of exovesicles of mitochondrial origin of the parasite in the serum of those affected by Chagas disease.

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Section: Introductionmentioning

confidence: 99%

Use of sera cell free DNA (cfDNA) and exovesicle-DNA for the molecular diagnosis of chronic Chagas disease

Lozano,

Samblas,

Calabuig

et al. 2023

PLoS ONE

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Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases

Pinheiro,

Torrecilhas,

Souza

et al. 2024

J of Extracellular Vesicle

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Parasitic diseases have a significant impact on human and animal health, representing a major hazard to the public and causing economic and health damage worldwide. Extracellular vesicles (EVs) have long been recognized as diagnostic and therapeutic tools but are now also known to be implicated in the natural history of parasitic diseases and host immune response modulation. Studies have shown that EVs play a role in parasitic disease development by interacting with parasites and communicating with other types of cells. This review highlights the most recent research on EVs and their role in several aspects of parasite‐host interactions in five key parasitic diseases: Chagas disease, malaria, toxoplasmosis, leishmaniasis and helminthiases. We also discuss the potential use of EVs as diagnostic tools or treatment options for these infectious diseases.

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Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

Cited by 2 publications

References 11 publications

Use of sera cell free DNA (cfDNA) and exovesicle-DNA for the molecular diagnosis of chronic Chagas disease

Use of sera cell free DNA (cfDNA) and exovesicle-DNA for the molecular diagnosis of chronic Chagas disease

Potential of extracellular vesicles in the pathogenesis, diagnosis and therapy for parasitic diseases

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