Identification and analysis of potential virulence-associated genes in Haemophilus parasuis based on genomic subtraction

Wang, Xiangru; Xu, Xiaojuan; Shu, Zhang; Guo, Fengjuan; Cai, Xuwang; Chen, Huanchun

doi:10.1016/j.micpath.2011.06.007

Cited by 15 publications

(18 citation statements)

References 19 publications

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“…Molecular study of virulence factors has revealed differences in the genome of the virulent and avirulent strains, indicating the need for a comprehensive analysis of H. parasuis isolates from different countries [7]. Several authors [8][9][10] have identified genes encoding potential virulence factors -the outer membrane transporter (vtaA, fhuA, hhdA, hhdB, nhaC, HAPS_0254, cirA) and adhesive proteins (sclB7, sclB11). Phage genes (phage_related) capable of encoding toxins are involved in the emergence of pathogenic microorganisms by a horizontal gene transfer [11].…”

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

“…To detect putative virulence genes, 10 pairs of primers [8][9][10] and temperature profiles similar to those described [8][9][10] were used. Reaction mixture comprised 0.4 μmol/l of each dNTP (Fermentas, Lithuania), 0.4 μmol/l primers (Syntol ZAO, Russia), 3 mmol/l MgCl 2 , 1 U Taq-polymerase, 1 Green Buffer (Promega, USA) and 5 μl template DNA.…”

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

“…Genes associated with virulence have been identified in the last few years due to suppression subtractive hybridization [8,16], representational difference analysis [10] and sequencing [9,17,18], but a relationship between the biological characteristics of isolates and the presence of these genes is still unclear. Here, after screening for virulence-associated genes, we compared biological and molecular genetic features found in this study to those published earlier for the reference strains.…”

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

“…Thus, searching genes responsible for virulence in H. parasuis is an urgent task, on which many researchers are working. Genetic engineering made it possible to identify putative genes that distinguish virulent strains from avirulent [8][9][10]. Unfortunately, the role of these genes in the pathogenesis is unclear.…”

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

“…When studying genes encoding virulence factors in 15 reference strains of H. parasuis [8][9][10], a correlation to virulence (p < 0.05) has been revealed for 8 genes -vtaA, fhuA, sclB11, nhaC, sclB7, HAPS_0254, hhdA and hhdB) [8][9][10]. Here, we studied 5 strains and 23 isolates detected in pigs with the signs of Glasser's disease from 13 Russian regions (see Table 2), and performed screening for 10 putative virulence loci.…”

Section: Putative Virulence Genes In Studied Haemophilus Parasuis Strmentioning

confidence: 99%

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DETERMINATION OF VIRULENCE FACTORS IN Haemophilus parasuis ISOLATES

Павелко¹,

Babin²,

Sprygin³

et al. 2016

S-h. biol.

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A b s t r a c tGlasser's disease is a bacterial infection typically characterized by fibrinous polyserositis, polyarthritis, meningitis, and occasionally acute pneumonia and septicemia. Haemophilus parasuis is the etiologic agent of Glasser's disease, leading to significant economic losses in swine industry. H. parasuis normally colonizes the upper respiratory tract of healthy pigs. Significant variation in virulence, serological and genetic properties has been shown across H. parasuis strains. However, stress, concomitant viral infections, or poor immune status allow H. parasuis to cause disease. The current literature contains a few studies that clarify differences in the genome between highly virulent and avirulent strains in the context of putative virulence factors. The objective of this study was to examine H. parasuis strains isolated on Russian pig farms during the 11-year study period (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011) for the presence of putative virulence loci. We screened 6 strains and 23 field isolates for the presence of 10 virulence factors. Identification of NAD-dependent isolates was performed by biochemical analysis and species-specific real-time PCR. Putative virulence genes were amplified by PCR using different primer pairs. Genetic analysis confirmed that the virulence factors (vtaA, fhuA, hhdA, hhdB, nhaC, HAPS_0254, sclB7, sclB11 and phage_related) exist in different combinations. Out of 28 strains studied, the vtaA gene was found in all strains, sclB7 and sclB11 genes were present in 24 and 25 strains respectively, the cirA gene in 18 out of 28, the fhuA gene in HAPS_0254, the hhdA gеne in 15, the hhdB gene in 14, the nhaC gene in 12, the phage_related target in only 3 strains. Our findings identified 15 novel genotypes (LUB, Ural'skii-DEP, MB, SK-1-DEP, SK3, Krasnodar, Botovo-2, Botovo-5, Botovo-7, IL2, AI4, V171, SH6, SW124, VN). The strains IL-1-DEP, KOMI DEP and isolate Nadeevo-2 carried all the loci, except for phage_related, which has only been reported for the virulent strains IA-84-17975, IA-84-22113 and SD-84-15995. In contrast to the paper by A.V. Potehin et al. (2007), IL-1-DEP turned out to be highly virulent, KOMI DEP exhibited moderately virulence, and Nadeevo-2 showed no virulence at all. Interestingly, the vtaA group 1 gene, the main determinant of virulence, was identified in SK-1-DEP avirulent strain and Botovo-2 and Nadeevo-2 avirulent isolates. This work contributes to a better understanding of putative virulence factors in field isolates of H. parasuis.

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Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

Section: Abstract: Haemophilus Parasuis Virulence Factors Glasser'smentioning

confidence: 99%

Section: Putative Virulence Genes In Studied Haemophilus Parasuis Strmentioning

confidence: 99%

See 3 more Smart Citations

DETERMINATION OF VIRULENCE FACTORS IN Haemophilus parasuis ISOLATES

Павелко¹,

Babin²,

Sprygin³

et al. 2016

S-h. biol.

View full text Add to dashboard Cite

show abstract

Comparative Transcriptomic Analyses of Haemophilus parasuis Reveal Differently Expressed Genes among Strains with Different Virulence Degrees

et al. 2021

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Identification of putative virulence-associated genes among Haemophilus parasuis strains and the virulence difference of different serovars

Zhang

et al. 2014

Microbial Pathogenesis

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Identification and analysis of potential virulence-associated genes in Haemophilus parasuis based on genomic subtraction

Cited by 15 publications

References 19 publications

DETERMINATION OF VIRULENCE FACTORS IN Haemophilus parasuis ISOLATES

DETERMINATION OF VIRULENCE FACTORS IN Haemophilus parasuis ISOLATES

Comparative Transcriptomic Analyses of Haemophilus parasuis Reveal Differently Expressed Genes among Strains with Different Virulence Degrees

Identification of putative virulence-associated genes among Haemophilus parasuis strains and the virulence difference of different serovars

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