Identification and analysis of circRNA–miRNA–mRNA regulatory network in hepatocellular carcinoma

Zhou, Daxiang; Dong, Ling Wang; Yang, Le-Min; Ma, Qiang; Liu, Feng; Li, Yanjie; Xiong, Shunbin

doi:10.1049/iet-syb.2020.0061

Cited by 16 publications

(13 citation statements)

References 42 publications

(40 reference statements)

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Section: Discussionmentioning

confidence: 99%

“…32,33 In a hepatocellular carcinoma study, NRAS, ITGA5, SEC14L2, SLC12A5, and SMAD2 were identified as prognostic factors that may affect patients through proteoglycans in cancer pathways. 34 We extracted exosomal RNA from BPE and MPE and identified 11 lncRNAs with the highest expression in the microarray results when analyzed using RT-qPCR, including LINC00452, AC137932. Tissue mechanics and metabolism is known to influence proliferation as well as migration and invasion, making them defining hallmarks of cancer.…”

Section: Discussionmentioning

confidence: 99%

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Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma

Huang

Zhou

Yang

et al. 2022

Clinical Laboratory Analysis

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Background Lung adenocarcinoma (LUAD) is a highly malignant tumor with a very low five‐year survival rate. In this study, we aimed to identify differentially expressed long‐chain non‐coding RNA (lncRNAs) and mRNAs from benign and malignant pleural effusion exosomes. Methods We used gene microassay and quantitative real‐time reverse transcription polymerase chain reaction (RT‐qPCR) to detect and verify differentially expressed mRNAs and lncRNAs in benign and malignant pleural effusion exosomes. Gene Ontology (GO) functional significance and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway significance enrichment analyses were performed to identify the difference in biological processes and functions between different mRNAs. We selected the lncRNA ZBED5‐AS1 with an upregulated differential fold of 3.003 and conducted a preliminary study on its cellular function. Results Gene microassay results revealed that 177 differentially expressed lncRNAs were upregulated, and 215 were downregulated. The top 10 upregulated were FMN1, AL118505.1, LINC00452, AL109811.2, CATG00000040683.1, AC137932.1, AC008619.1, AL450344.1, AC092718.6, and ZBED5‐AS1. The top 10 downregulated were TEX41, G067726, JAZF1‐AS1, AC027328.1, AL445645.1, AL022345.4, AC008572.1, AC123777.1, AC093714.1, and PHKG1. For the mRNAs, 79 were upregulated, and 123 were notably downregulated. GO analysis revealed that the upregulated differential mRNAs were mainly involved in “cellular response to acidic pH” (biological processes), “endoplasmic reticulum part” (cellular components), and “at DNA binding, cyclase activity” (molecular functions). KEGG pathways were found to be related to V. cholerae infection, Parkinson's disease, and cell adhesion molecules. RT‐qPCR showed that ZBED5‐AS1 was highly expressed in LUAD tissues, cells, and benign and malignant pleural fluid exosomes. Overexpression of ZBED5‐AS1 could significantly promote the proliferation, migration, invasion, and colony formation of LUAD cells, and knockdown had the opposite consequence. Conclusion The pleural effusion exosomes from patients with LUAD include several improperly expressed genes, and lncRNA‐ZBED5‐AS1 is a new biomarker that aids in our understanding of the occurrence and progression of LUAD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma

Huang

Zhou

Yang

et al. 2022

Clinical Laboratory Analysis

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“…miRNA-211 can regulate the MAPK3 pathway negatively, so it is in keeping with its role in this pathway that MT reduced miR-211 in the present study. CircRNA can compete with miRNA to regulate gene expression[69]and is a component of circRNA/miRNA/mRNA involved in regulatory networks as diverse as hepatocellular carcinoma (HCC), contraction of skeletal muscle [70] and proliferation of myoblasts [71]. Differentially expressed circRNAs have been studied in hair follicles of Cashmere goats [72] and Angora rabbits [73].…”

Section: Discussionmentioning

confidence: 99%

Melatonin regulates the mitogen-activated protein kinase ( MAPK ) pathway via the circMPP5-miR-211-MAPK3 axis to induce secondary hair follicle differentiation and development in newborn Cashmere goat kids

Diao

Yao

Duan

et al. 2022

Preprint

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Background The quality and yield of Cashmere fibre are closely related to the differentiation and development of secondary hair follicles in the skin of Cashmere goats. The higher the density of secondary hair follicles, the higher the quality and yield of Cashmere from the fleece. Development of secondary hair follicles commences in the embryonic stage of life and is completed six months after birth. Preliminary experimental results from our laboratory showed that melatonin (MT) treatment of goat kids after their birth could increase the density of secondary hair follicles and, thus, improve the subsequent yield and quality of Cashmere. These changes in the secondary hair follicles resulted from increases in levels of antioxidant and expression of anti-apoptotic protein, and from a reduction in apoptosis. The present study was conducted to explore the molecular mechanism of MT-induced secondary hair follicle differentiation and development by using whole-genome analysis. Results MT had no adverse effect on the growth performance of Cashmere kids but significantly improved the character of the secondary hair follicles and the quality of Cashmere, and this dominant effect continued to the second year. Melatonin promotes the proliferation of secondary hair follicle cells at an early age. Secondary hair follicles in the MT group formation was earlier than in the control group in the second year. The genome-wide data results involved KEGG analysis of 1044 DEmRNAs, 91 DElncRNAs, 1054 DEcircRNAs, and 61 DEmiRNAs which revealed that the MAPK signaling pathway is involved in the development of secondary hair follicles, with key genes (FGF2, FGF21, FGFR3, MAPK3 (ERK1)) being up-regulated and expressed. We also found that the circMPP5 could combine with miR-211 and regulate the expression of MAPK3. Conclusions We conclude that MT achieves its effects by regulating the MAPK pathway through the circMPP5-miR-211-MAPK3 axis to induce the differentiation and proliferation of secondary hair follicle cells. In addition there is up-regulation of expression of the anti-apoptotic protein causing reduced apoptosis of hair follicle cells. Collectively, these events increase the numbers of secondary hair follicles, thus improving the production of Cashmere from these goats.

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“…CircSIAE (hsa_circ_0000367) is a circRNA formed by reverse splitting of linear SIAE gene at the position chr11:124517260-124518071 on chromosome, consisting of 244 nucleotides. It is derived from a spline of SIAE mRNA associated with immune diseases and reported as a novel potential biomarker for emerging rheumatoid arthritis ( Luo et al., 2020 ; Zhou et al., 2020 ). In this study, we used cultured cell systems to study the effect of CVB3 infection on expression levels of circRNAs and to investigate potential downstream mechanisms of their involvement in viral processes.…”

Section: Introductionmentioning

confidence: 99%

The circRNA circSIAE Inhibits Replication of Coxsackie Virus B3 by Targeting miR-331-3p and Thousand and One Amino-Acid Kinase 2

Yang

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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Coxsackie virus B3 (CVB3), an enterovirus, is the main pathogen causing viral myocarditis, pericarditis, hepatitis and other inflammation-related diseases. Non-coding RNAs with a closed loop molecular structure, called circular RNAs (circRNAs), have been shown to be involved in multiple virus-related processes, but roles and mechanisms in CVB3 infection have not been systematically studied. In this study, when HeLa cells were infected with CVB3, the expression of hsa_circ_0000367 (circSIAE) was significantly decreased as demonstrated by real-time quantitative PCR assays. We found that circSIAE downregulated the expression of miR-331-3p through direct binding and inhibited the replication of CVB3 in HeLa and 293T cells. The analysis of signals downstream of miR-331-3p suggested that miR-331-3p promotes CVB3 replication, viral plaque formation and fluorescent virus cell production through interactions with the gene coding for thousand and one amino-acid kinase 2 (TAOK2). In conclusion, this study found that circSIAE can target TAOK2 through sponge adsorption of miR-331-3p to inhibit the replication and proliferation of CVB3 virus, providing an early molecular target for the diagnosis of CVB3 infection.

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Identification and analysis of circRNA–miRNA–mRNA regulatory network in hepatocellular carcinoma

Cited by 16 publications

References 42 publications

Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma

Construction and analysis of expression profile of exosomal lncRNAs in pleural effusion in lung adenocarcinoma

Melatonin regulates the mitogen-activated protein kinase ( MAPK ) pathway via the circMPP5-miR-211-MAPK3 axis to induce secondary hair follicle differentiation and development in newborn Cashmere goat kids

The circRNA circSIAE Inhibits Replication of Coxsackie Virus B3 by Targeting miR-331-3p and Thousand and One Amino-Acid Kinase 2

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