Identification and Activity of Cytosol Creatine Phosphokinase Enzymes in Normal and Diseased Skin

Zemtsov, Alexander; Cameron, Greg; Bradley, Charles; Montalvo-Lugo, Victor; Mattioli, Federico

doi:10.1097/00000441-199412000-00011

Cited by 9 publications

(17 citation statements)

References 26 publications

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“…We were also able to demonstrate, as was suggested by Spizz et al and Kim et al (15,16), that growth factor application can cause a cytosol CPK MM to CPK BB transition. I believe these data confirm our work (5) and the work of Scandinavian investigators (13,14), who observed the presence of both CPK BB and MM in the skin, and the relative distribution of CPK, MM, and BB was dependent on skin pathology (5) (furthermore, we have shown in our prior publication that in sacrificed mice keratinocytes CPK activity is primarily due to the MM isoenzyme, but in mouse keratinocyte culture, where growth factors are present, the activity was primarily toward CPK BB) (5). We and Scandinavian investigators (5,13,14) studied CPK by a clinically available electrophoresis methodology detecting both CPK MM and CPK BB, while Schlattner et al (7), utilizing immunohistochemical and molecular biology techniques, primarily detected CPK BB isoenzyme in skin.…”

Section: Discussionsupporting

confidence: 91%

“…Scandinavian investigators reported that both CPK MM and CPK BB were found in epidermal suction blister fluid (13, 14). We observed very similar results in all our human specimens (5). Both CPK BB and CPK MM were present (but only a trace amount of CPK MB).…”

supporting

confidence: 86%

“…The epidermis was scraped from the skin (the presence of keratinocytes and the absence of panniculus carnosus, a thin layer of skeletal muscle present in the subcutaneous layer of murine skin, was assessed histologically). CPK enzyme extraction analysis was performed as described previously by our research group (5). The tissue was homogenized (1 g in 10 mL) in Tris hydrochloride buffer with sodium chloride and dithiothreitol.…”

Section: Cpk Measurementmentioning

confidence: 99%

“…Furthermore, the relative distribution of CPK MM and CPK BB isoenzyme was dependent on skin pathology (5). For example, in psoriatic plaques, the majority of CPK activity was due to BB isoenzyme, but in uninvolved psoriatic skin the majority of CPK activity was due to MM isoenzyme (5). Furthermore, more recently, we became aware that growth factors can induce CPK MM to CPK BB transition (15, 16).…”

mentioning

confidence: 99%

“…Both CPK BB and CPK MM were present (but only a trace amount of CPK MB). Furthermore, the relative distribution of CPK MM and CPK BB isoenzyme was dependent on skin pathology (5). For example, in psoriatic plaques, the majority of CPK activity was due to BB isoenzyme, but in uninvolved psoriatic skin the majority of CPK activity was due to MM isoenzyme (5).…”

mentioning

confidence: 99%

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Topically applied growth factors change skin cytoplasmic creatine kinase activity and distribution and produce abnormal keratinocyte differentiation in murine skin

Zemtsov

Montalvo-Lugo

2008

Skin Research and Technology

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Section: Discussionsupporting

confidence: 91%

supporting

confidence: 86%

Section: Cpk Measurementmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Topically applied growth factors change skin cytoplasmic creatine kinase activity and distribution and produce abnormal keratinocyte differentiation in murine skin

Zemtsov

Montalvo-Lugo

2008

Skin Research and Technology

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Creatine Kinase and Creatine Transporter in Normal, Wounded, and Diseased Skin

Schlattner

Möckli

Speer

et al. 2002

Journal of Investigative Dermatology

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Skin comprises many cell types that are characterized by high biosynthetic activity and increased energy turnover. The creatine kinase system, consisting of creatine kinase isoenzymes and creatine transporter, is known to be important to support the high energy demands in such cells. We analyzed the presence and the localization of these proteins in murine and human skin under healthy and pathologic conditions, using immunoblotting and confocal immunohistochemistry with our recently developed specific antibodies. In murine skin, we found high amounts of brain-type cytosolic creatine kinase coexpressed with lower amounts of ubiquitous mitochondrial creatine kinase, both mainly localized in suprabasal layers of the epidermis, different cell types of hair follicles, sebaceous glands, and the subcutaneous panniculus carnosus muscle. With exception of sebaceous glands, these cells were also expressing creatine transporter. Muscle-type cytosolic creatine kinase and sarcomeric mitochondrial creatine kinase were restricted to panniculus carnosus. Immediately after wounding of murine skin, brain-type cytosolic creatine kinase and a creatine transporter-subspecies were transiently upregulated about 3-fold as seen in immunoblots, whereas the amount of ubiquitous mitochondrial creatine kinase increased during days 10-15 after wounding. Healthy and psoriatic human skin showed a similar coexpression pattern of brain-type cytosolic creatine kinase, ubiquitous mitochondrial creatine kinase, and creatine transporter in this pilot study, with creatine transporter species being upregulated in psoriasis.

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High-throughput metabolome comparison of cutaneous squamous cell carcinoma, basal cell carcinoma, and healthy skin with e-biopsy sampling

Louie,

Wise,

Berl

et al. 2024

Preprint

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A standard histopathology-slides based diagnostics becomes a serious process bottleneck due to rising incidence rates of cutaneous squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC). Leveraging tissue molecular information for diagnostics can be a beneficial alternative in certain cases. Sampling and processing of a constantly growing number of tumors can be enhanced with faster specimen collection methods together with high-throughput molecular identification approaches. Tumor specimens can be collected with electroporation-based biopsy (e-biopsy), a minimally invasive sampling collection tool with a proven ability, while mass spectrometry can be used for molecular identification. The aim of this study was (i) to confirm the ability of e-biopsy technique to harvest metabolites, (ii) to obtain high-resolution metabolomic profiles of cSCC, BCC, and healthy skin tissues, and (iii) to perform a comparative analysis of the collected profiles. Data, collected with e-biopsy coupled with ultra performance liquid chromatography and tandem mass spectrometry (UPLC-MS-MS), expands the current metabolomic profiles reported for cSCC, BCC, and healthy skin. Here we report measurements of 2325 small metabolites identified (301 with high confidence) in 13 tissue samples from 12 patients. Comparative analysis identified 34 significantly (p<0.05) differentially expressed high-confidence metabolites. Generally, we observed a greater number of metabolites with higher expression, in cSCC and in BCC compared to healthy tissues, belonging to the subclass amino acids, peptides, and analogues.

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Identification and Activity of Cytosol Creatine Phosphokinase Enzymes in Normal and Diseased Skin

Cited by 9 publications

References 26 publications

Topically applied growth factors change skin cytoplasmic creatine kinase activity and distribution and produce abnormal keratinocyte differentiation in murine skin

Topically applied growth factors change skin cytoplasmic creatine kinase activity and distribution and produce abnormal keratinocyte differentiation in murine skin

Creatine Kinase and Creatine Transporter in Normal, Wounded, and Diseased Skin

High-throughput metabolome comparison of cutaneous squamous cell carcinoma, basal cell carcinoma, and healthy skin with e-biopsy sampling

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