Identification among morphologically similar Argyreia (Convolvulaceae) based on leaf anatomy and phenetic analyses

Abstract

BackgroundThe genus Argyreia Lour. is one of the species-rich Asian genera in the family Convolvulaceae. Several species complexes were recognized in which taxon delimitation was imprecise, especially when examining herbarium materials without fully developed open flowers. The main goal of this study is to investigate and describe leaf anatomy for some morphologically similar Argyreia using epidermal peeling, leaf and petiole transverse sections, and scanning electron microscopy. Phenetic analyses including cluster analysis and principal component analysis were used to investigate the similarity of these morpho-types.ResultsAnatomical differences observed between the morpho-types include epidermal cell walls and the trichome types on the leaf epidermis. Additional differences in the leaf and petiole transverse sections include the epidermal cell shape of the adaxial leaf blade, the leaf margins, and the petiole transverse sectional outline. The phenogram from cluster analysis using the UPGMA method represented four groups with an R value of 0.87. Moreover, the important quantitative and qualitative leaf anatomical traits of the four groups were confirmed by the principal component analysis of the first two components. The results from phenetic analyses confirmed the anatomical differentiation between the morpho-types.ConclusionsLeaf anatomical features regarded as particularly informative for morpho-type differentiation can be used to supplement macro morphological identification.

Pollination and Floral Biology of a Rare Morning Glory Species Endemic to Thailand, Argyreia siamensis

“…Three of these spirit specimens were examined with a scanning electron microscope (Hitachi SU8010) [38,109] to study the surface of the floral nectary. The other three spirit specimens were examined via anatomical techniques using the paraffin method [89,[110][111][112]. Briefly, the paraffin method requires all water to be removed from the spirit specimens by first using a vacuum and then dehydrating the tissue with tert-Butyl alcohol (TBA) series [89,[110][111][112].…”

Phenetic Kinship Relationship of Apocynaceae Family Based on Morphological and Anatomical Characters

“…Figure 3a shows the presence of guard cells and neighbouring cells at a magnification of 12.5x10. According to (Mulyani, 2006) explains that in stomata, two special cells limit the stomata, called guard cells, and some (Traiperm et al, 2017). This is in line with (Resmi, 2019) in a journal entitled Stomatal Types In Monocot And Dicot Plants Within Flora Of Nirmala College Campus explained that research on 40 plants collected, 16 plants showed anomocytic stoma, one of which was from the Apocynaceae family, Tabernaemontana sp.…”

A threatened new species of Ipomoea (Convolvulaceae) from the Brazilian Cerrado revealed by morpho-anatomical analysis

“…In Convolvulaceae, these characters have also been consistent and informative, such as the type of mesophyll that was used to delimit three species of Evolvulus (Ketjarun et al 2016), the shape of the main vein and vascular bundles used to clarify the relationship between morphologically similar Argyrea species (Traiperm et al 2017). Furthermore, anatomical characters strongly supported species identification in an investigation of Merremia section Xanthips (Pisuttimarn et al 2013).…”

“…These characters are considered consistent (Metcalfe and Chalk 1979) and useful for Convolvulaceae taxonomy (e.g. Ketjarun et al 2016;Traiperm et al 2017). Ipomoea maranhensis can also be compared to I. langsdorffii Choisy, an endemic species from the Southeastern region of Brazil occuring in the Cerrado and Atlantic forest, due to its oblong leaves and flowers in dichasia.…”

“…To support the morphological delimitation between this morphotype and I. burchellii, we performed a comparative anatomical analysis of the leaf, as this has been used to support the morphological delimitation of species in various plant groups (Lersten 1974;Gomes et al 2005;Rio et al 2005;Oliveira et al 2011;Thadeo et al 2014), as well as in Convolvulaceae (Ketjarun et al 2016;Traiperm et al 2017). Thus, considering the taxonomic alleged consistency of the anatomical characters analyzed in Convolvulaceae (Metcalfe and Chalk 1979), along with the morphological discontinuities found, we describe this morphotype as a new species.…”