Identificación molecular de Entamoeba histolytica, Entamoeba dispar y Entamoeba moshkovskii en niños con diarrea en Maracaibo, Venezuela

Rivero, Zulbey; Villareal, Lisbeth; Bracho, Ana; Prieto, Carem; Villalobos, Rafael

doi:10.7705/biomedica.5584

Cited by 4 publications

References 31 publications

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A commensal protozoan attenuates Clostridioides difficile pathogenesis in mice via arginine-ornithine metabolism and host intestinal immune response

Yang,

Wu,

et al. 2024

Nat Commun

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Antibiotic-induced dysbiosis is a major risk factor for Clostridioides difficile infection (CDI), and fecal microbiota transplantation (FMT) is recommended for treating CDI. However, the underlying mechanisms remain unclear. Here, we show that Tritrichomonas musculis (T.mu), an integral member of the mouse gut commensal microbiota, reduces CDI-induced intestinal damage by inhibiting neutrophil recruitment and IL-1β secretion, while promoting Th1 cell differentiation and IFN-γ secretion, which in turn enhances goblet cell production and mucin secretion to protect the intestinal mucosa. T.mu can actively metabolize arginine, not only influencing the host’s arginine-ornithine metabolic pathway, but also shaping the metabolic environment for the microbial community in the host’s intestinal lumen. This leads to a relatively low ornithine state in the intestinal lumen in C. difficile-infected mice. These changes modulate C. difficile’s virulence and the host intestinal immune response, and thus collectively alleviating CDI. These findings strongly suggest interactions between an intestinal commensal eukaryote, a pathogenic bacterium, and the host immune system via inter-related arginine-ornithine metabolism in the regulation of pathogenesis and provide further insights for treating CDI.

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A commensal protozoan attenuates Clostridioides difficile pathogenesis in mice via arginine-ornithine metabolism and host intestinal immune response

Yang,

Wu,

et al. 2024

Nat Commun

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A murine commensal protozoan relievesClostridium difficileinfection through regulating the arginine metabolism and the host intestinal immune response

Yang

Zang

et al. 2023

Preprint

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Antibiotic-induced dysbiosis is a key predisposing factor for Clostridium difficile infection (CDI), fecal microbiota transplantation (FMT) is recommended for treating CDI. However, the mechanisms remain unclear. Here, we uncover that one integral member of the murine gut microbiota, a commensal protozoan from mice, Tritrichomonas musculis (T. mu), that reduced intestinal damage by inhibiting the recruitment of neutrophil and secretion of IL-1β, and increased Th1 cell differentiation and secretion of IFN-γ, which increased the goblet cell and secretion of mucin to protect the intestinal mucosa. The T. mu upregulated the key enzymes of arginine metabolism iNOS, ASS, OTC and down-regulated ARG1 in CDI mice, resulting in an increase in arginine, citrulline and a decrease in ornithine, which worked together to regulate the host intestinal immune response and alleviate CDI. This work reveals the interaction mechanism between intestinal commensal eukaryotes and pathogenic bacteria, and also provide new ideas for treating CDI.

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Parasitosis intestinales en niños del cantón Ambato, Ecuador

De la Torre-Fiallos,

Pacha-Jara,

Caiza-Vega

2023

Med. Lab.

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Introducción. Las parasitosis intestinales siguen siendo un grave problema de salud pública en especial para la población infantil. El objetivo del presente estudio fue determinar la prevalencia de parasitosis intestinal en niños de 5 a 9 años de edad de la Parroquia de Totoras del cantón Ambato. Metodología. Se trata de un estudio observacional, descriptivo, prospectivo y transversal, donde se seleccionaron 153 niños mediante muestreo probabilístico, a quienes se les solicitó una muestra de materia fecal, a la cual se le realizó un examen directo (con solución salina y Lugol) y la técnica de flotación de Willis. Resultados. Se obtuvo un 77,1 % de niños parasitados, predominando el poliparasitismo (77 %) sobre el monoparasitismo (23 %) y los cromistas/protozoarios sobre los helmintos (91,7 % versus 8,3 %). Las principales especies encontradas fueron: Blastocystis spp. (47,6 %), Entamoeba coli (32,03 %) y Endolimax nana (28,1 %). Conclusiones. Se concluye que los niños de la Parroquia de Totoras tienen una alta frecuencia de parasitosis intestinal, por lo que es importante realizar campañas de prevención y tratamiento antiparasitario en esta comunidad de niños, con el fin de disminuir la prevalencia de estos agentes microbianos.

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Identificación molecular de Entamoeba histolytica, Entamoeba dispar y Entamoeba moshkovskii en niños con diarrea en Maracaibo, Venezuela

Cited by 4 publications

References 31 publications

A commensal protozoan attenuates Clostridioides difficile pathogenesis in mice via arginine-ornithine metabolism and host intestinal immune response

A commensal protozoan attenuates Clostridioides difficile pathogenesis in mice via arginine-ornithine metabolism and host intestinal immune response

A murine commensal protozoan relievesClostridium difficileinfection through regulating the arginine metabolism and the host intestinal immune response

Parasitosis intestinales en niños del cantón Ambato, Ecuador

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