Identificação de bergenina e carotenóides no fruto de uchi (Endopleura uchi, Humiriaceae)

Abstract

RESUMOO extrato obtido em metanol da polpa de frutos Endopleura uchi foi submetido a fracionamento utilizando-se técnicas cromatográficas convencionais levando ao isolamento de bergenina. Na análise da composição dos carotenóides foi evidenciada a predominância de β-caroteno (16,57 μg.g -1 ). Os isômeros do β-caroteno foram detectados, trans-β-caroteno (89,3%), 13-cis-β-caroteno (8%) e 9-cis-β-caroteno (3%). Considerando a importância do papel nutricional dos carotenóides e a bioatividade do glicosídeo bergenina, esse estudo sugeriu o potencial desse fruto como alimento funcional. PALAVRAS-CHAVEEndopleura uchi, Bergenina, β-Caroteno. Identification of bergenin and carotenoids in the uchi (Endopleura uchi, Humiriaceae) fruit ABSTRACTThe extract obtained in methanol from the fruits pulp of Endopleura uchi was fractionated using conventional chromatographic tecniques to isolate bergenin. In the analysis of the composition of carotenoids was evidenced the predominance of β-carotene (16.57 μg.g -1 ). The isomers of β-carotene: trans-β-carotene (89.3%), 13-cis-β-carotene (8%) and 9-cis-β-carotene (3%) were detected. Considering the importance of the nutritional role of carotenoids and the bioactivity of the bergenina, this study suggested the potential of this fruit as functional food.

Characterization of bergenin in Endopleura uchi bark and its anti-inflammatory activity

“…The pulp of the fruit has a high content of fat, predominantly oleic acid 4 and carotenoids, mostly trans-β-carotene. 5 Bark tea of E. uchi is used in the traditional medicine as an anti-inflammatory and against tumors and uterine infections, 6,7 and we have focused on the chemistry of this bark. We isolated bergenin, a C-glycoside of 4-O-methyl gallic acid, from E. uchi bark.…”

“…We isolated bergenin, a C-glycoside of 4-O-methyl gallic acid, from E. uchi bark. Previously bergenin has been isolated from E. uchi fruits, 5 and another Humiriaceae species, Humiria balsamifera Aubl. 8 and Sacoglottis gabonensis.…”

Antimicrobial activity of bergenin from Endopleura uchi (Huber) Cuatrec

“…There are few chemical studies of E. uchi and are concentrated in the fruits that are very appreciated in the region. The pulp of the fruit has a high content of fat, predominantly oleic acid (Marx et al, 2002) and carotenoids, mostly trans-β-carotene (Magalhães et al, 2007). However is the bark tea of E. uchi that is used in the traditional medicine as an anti-inflammatory, also against tumors, and uterine infections (Corrêa, 1984, Revilla, 2001.…”

“…We isolated bergenin, a derivative of C-glycoside of 4-O-methyl gallic acid, from the bark. Previously bergenin had also been isolated from E. uchi fruits (Magalhães et al, 2007) and also many other species, including species of the family as Humiria balsamifera (Caldas et al, 2002) and Sacoglottis gabonensis (Ogan, 1971). Bergenin presents several biological activities such as anti-inflammatory (Swarnalakshmi et al, 1984;Nazir et al, 2007), antioxidant (Sumino et al, 2002), hepatoprotective (Lim et al, 2000), neuroprotective (Takahachi et al, 2003), and anti-HIV (Piacente, 1996).…”

Aplicação de baixas doses de radiação ionizante no fruto brasileiro tucumã (Astrocarium vulgare Mart.)

“…Os carotenóides foram extraídos com acetona e em seguida sofreram partição para éter de petróleo, sendo concentrados, por meio da evaporação do solvente sob fluxo de nitrogênio, e diluídos em acetona 1 μL (Lima et al 2004;Magalhães et al 2007). A fase móvel foi transferida para balão volumétrico (100 mL) através de funil com sulfato de sódio anidro e quantificados por espectrofotometria a 450 nm.…”