Identificação de Bactérias Causadoras de Infecção Hospitalar Utilizando Fenotipagem  Clássica / Identification of Hospital Infectious Bacteria Using Classical Phenotyping

Andrade, Carolina Rodrigues; Filho, Arlindo Rodrigues Galvão; Costa, Aline C. M. M.; Oliveira, Thaís Alves de; Carneiro, Lí­lian Carla; Avelino, Melissa Ameloti Gomes

doi:10.34117/bjdv7n6-036

Cited by 3 publications

(5 citation statements)

References 20 publications

(20 reference statements)

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“…Results previously published by our group [ 22 ] showed that 39.4% and 42.4% of S. haemolyticus species were resistant to penicillin G and oxacillin, respectively. S. auricularis exhibited values of 15.2% for both tested antibiotics and S. intermedius of 12.1% for both antibiotics.…”

Section: Resultsmentioning

confidence: 90%

“…The isolates used in this study were collected, identified, and tested for antibiotic susceptibility in the study conducted by Rodrigues and collaborators [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…After the analyses, all samples were kept in a freezer at −80 °C in tubes containing casein-soy broth and 10% glycerol until the moment of reactivation for the molecular analysis of the present study. Thus, in order to complement the results previously published by our group [ 22 ], we evaluated the molecular expression of the blaZ , femA , and mecA genes that are associated with the acquisition of resistance mechanisms against the action of beta-lactams.…”

Section: Methodsmentioning

confidence: 99%

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Staphylococcus spp. Causatives of Infections and Carrier of blaZ, femA, and mecA Genes Associated with Resistance

et al. 2023

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Staphylococcus spp. have been associated with cases of healthcare associated infections due to their high incidence in isolates from the hospital environment and their ability to cause infections in immunocompromised patients; synthesize biofilms on medical instruments, in the case of negative coagulase species; and change in genetic material, thus making it possible to disseminate genes that code for the acquisition of resistance mechanisms against the action of antibiotics. This study evaluated the presence of blaZ, femA, and mecA chromosomal and plasmid genes of Staphylococcus spp. using the qPCR technique. The results were associated with the phenotypic expression of resistance to oxacillin and penicillin G. We found that the chromosomal femA gene was present in a greater proportion in S. intermedius when compared with the other species analyzed, while the plasmid-borne mecA gene was prevalent in the S. aureus samples. The binary logistic regression performed to verify the association among the expression of the genes analyzed and the acquisition of resistance to oxacillin and penicillin G were not significant in any of the analyses, p > 0.05.

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Section: Resultsmentioning

confidence: 90%

“…The isolates used in this study were collected, identified, and tested for antibiotic susceptibility in the study conducted by Rodrigues and collaborators [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Staphylococcus spp. Causatives of Infections and Carrier of blaZ, femA, and mecA Genes Associated with Resistance

et al. 2023

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“…Several bacterial species are capable of spreading in the hospital environment, and according to Oliveira (2008) (Andrade et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Tracking of Microorganisms on the Surface and in Servers a the Public Hospital in Palmas-To

Nunes da Silva,

Cavalcante Varão,

Bezerra de Carvalho

et al. 2024

DRIUFT

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Hospital infection (HI) is a public health problem in Brazil. Resistant bacteria increase the risk of superinfections and raise the number of deaths, factors that may be associated with failures in antiseptic and environmental disinfection processes, favoring cross-contamination. This study investigated the presence of microorganisms on hospital surfaces, on employees' hands and nostrils, and analyzed retrospective infections suspected of hospital infection at a Public Hospital in Palmas, TO. The results revealed the predominant presence of microorganisms of the genus Staphylococcus. The analysis indicated shortcomings in antiseptic and disinfection procedures, highlighting the urgent need for more rigorous biosafety measures. The research emphasizes the importance of appropriate antimicrobial protocols to prevent and treat Healthcare-Associated Infections (HAIs) and underscores the need for awareness and training of healthcare professionals. The study also points to the growing antimicrobial resistance and frequent identification of multidrug-resistant strains. These findings have significant implications for improving patient safety in hospital settings and provide a solid foundation for the development of effective strategies for the prevention and control of nosocomial infections, aiming to reduce the incidence of HI and enhance the quality of healthcare.

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“…To prevent and reduce the risks of contamination, biosecurity measures must be implanted and followed. Measures such as the adoption of rules, secure methods that are appropriated to the maintenance of the health of patients, professionals, and visitors, which help prevent the dissemination of infectious agents, therefore decreasing the risk of developing a nosocomial infection, especially bacterial infections: Stenotrophomonas maltophilia, Klebsiella pneumoniae, Escherichia coli, Streptococcus pneumoniae, Haemophilus influenzae, Acinetobacter baumannii and Staphylococcus aureus, and they may present resistance to several antimicrobials (Zhong, 2020;Andrade, 2021).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of multi-resistant bacteria, environmental contamination, and routine practices in a public hospital

Puzi

Almeida²,

Leão³

et al. 2022

RSD

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The significant increase in antimicrobial-resistant bacteria is a major public health problem. The Intensive Care Units (ICUs), as they have critically ill patients, are the most susceptible and promoters of hospital infections. Acinetobacter baumannii and Klebsiella pneumoniae are important species that cause hospital infections, associated with resistance mechanisms that make the strains more virulent. The purpose of this article was to perform epidemiological tracking of the most prevalent bacterial strains and seek their involvement with hospital infections in a public hospital in Palmas, Tocantins, Brazil. A retrospective study of nosocomial infections from 2017 to 2019 was carried out. In 2019, screening was carried out on bed surfaces, using a sterile swab. In 2020, suspected cases of hospital infection were registered. The samples were sown in specific media and evaluated the resistance profile. Results: a higher prevalence of A. baumannii was found in Intensive Care Units (70.4%) in the respiratory tract and on surfaces. K. pneumoniae showed a significant increase in the ICU and ICU in 2018 and 2019. Professional practices that could contribute to cross-contamination were observed during collections, with little antisepsis and lack of personal protective equipment being identified. The results reinforce the need to create mechanisms for improvement and continuing education for professionals, as well as effective antisepsis processes, and creating outbreak investigation strategies.

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Identificação de Bactérias Causadoras de Infecção Hospitalar Utilizando Fenotipagem Clássica / Identification of Hospital Infectious Bacteria Using Classical Phenotyping

Abstract: Este estudo teve como objetivo identificar os microrganismos presentes nas mãos da equipe multiprofissional alocados em Unidade de Terapia Intensiva (UTI), e seu papel nas IRAS.

Cited by 3 publications

References 20 publications

Staphylococcus spp. Causatives of Infections and Carrier of blaZ, femA, and mecA Genes Associated with Resistance

Staphylococcus spp. Causatives of Infections and Carrier of blaZ, femA, and mecA Genes Associated with Resistance

Tracking of Microorganisms on the Surface and in Servers a the Public Hospital in Palmas-To

Prevalence of multi-resistant bacteria, environmental contamination, and routine practices in a public hospital

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